Modulation of gene expression by thymoquinone conjugated zinc oxide nanoparticles arrested cell cycle, DNA damage and increased apoptosis in triple negative breast cancer cell line MDA-MB-231

Sjs, Banupriya; Kavithaa, Krishnamoorthy; Arumugam, Ponnan; Perumalsamy, Haribalan; B, Sri Renukadevi; Sumathi, S.

doi:10.1080/03639045.2022.2072513

Cited by 6 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the 24th hour, they showed that it caused higher toxicity in tumor cells . In another study, they coated TQ with a zinc oxide nanoparticle in the MDA-MB231 cell line, examined its effect on breast cancer cells, and showed by the flow cytometric method that it stopped DNA damage and cell cycle and increased apoptosis . Ibiyeye et al coated TQ with a CaCO 3 nanoparticle and investigated its antiproliferative effect on the same tumor cell line (MDA-MB231 cell line) and found that the IC 50 value of the TQ-CaCO 3 nanoparticle was higher than that of TQ.…”

Section: Discussionmentioning

confidence: 99%

“… 38 In another study, they coated TQ with a zinc oxide nanoparticle in the MDA-MB231 cell line, examined its effect on breast cancer cells, and showed by the flow cytometric method that it stopped DNA damage and cell cycle and increased apoptosis. 39 Ibiyeye et al coated TQ with a CaCO 3 nanoparticle and investigated its antiproliferative effect on the same tumor cell line (MDA-MB231 cell line) and found that the IC 50 value of the TQ-CaCO 3 nanoparticle was higher than that of TQ. However, the combination therapy showed improved apoptosis, reduced intracellular migration, and reduced invasion when compared with single drug-loaded nanoparticles and free drugs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Exploring the Potential of Thymoquinone-Stabilized Selenium Nanoparticles: In HEC1B Endometrial Cancer Cells Revealing Enhanced Anticancer Efficacy

Gulbay,

Secme,

Ilhan

2023

ACS Omega

View full text Add to dashboard Cite

The aim of this research is to examine the potential anticancer properties of thymoquinone (TQ)-encapsulated selenium nanoparticles (TQ-SeNPs) in HEC1B endometrial carcinoma cells. TQ-SeNPs were synthesized, and their size, morphology, and elemental analysis were characterized. Morphological changes were examined by using scanning electron microscopy (SEM). The cytotoxicity and viability of nanothymoquinone were assessed by the XTT (2,3-bis (2-methoxy-4-nitro-5sulfophenyl)-2H-tetrazolium-5 carboxanilide) assay. Gene expressions and protein levels of the mitogen-activated protein kinase (MAPK) signaling pathway were analyzed by real-time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. The decrease in the viability of HEC1B endometrial carcinoma cells was observed in a time-and dose-dependent manner. HEC-1B cells were treated with TQ-SeNP at 40−640 μg/mL concentrations and time intervals, and their viability was assessed by XTT assay. IC 50 doses of TQ-SeNP in HEC1B cells were detected as 526.45 μg/mL at 48th hour. ELISA indicated that TQ-SeNP treatment reduced the level of p38 MAPK. ERK2, MEK2, and NFKB (p65) mRNA expressions were decreased in the dose group administered TQ-SeNP at the 48th hour compared to that in the control group. However, it was not significant. The novel nanoparticle showed an antiproliferative effect in endometrial cancer cells. However, further studies are needed to increase the anticancer activity of the cell in the TQ-SeNP interaction.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exploring the Potential of Thymoquinone-Stabilized Selenium Nanoparticles: In HEC1B Endometrial Cancer Cells Revealing Enhanced Anticancer Efficacy

Gulbay,

Secme,

Ilhan

2023

ACS Omega

View full text Add to dashboard Cite

show abstract

“…Ruenraroengsak et al used ZnONPs combined with anti-FZD-7 antibodies to target and kill highly expressing FZD-7 breast cancer cells, highlighting the potential clinical application value of this nanoplatform [ 107 ]. Recently, Sjs et al combined Thymoquinone (TQ) with ZnONPs to yield TQ-ZnO nanoparticles that inhibited breast cancer cell proliferation, increased DNA damage, and led to apoptosis in the TNBC cell line MDA-MB-231 [ 108 ]. In addition to chemotherapy and targeted immunotherapy, ZnO was used for the synthesis of chitosan-ZnO biological nanocomposites (CS-ZnO BNCs) as radiosensitizers to enhance the effect of radiation therapy in breast cancer models, reflecting advantages in radiation therapy [ 109 ].…”

Section: Zinc Oxide Nanoparticles and Breast Cancermentioning

confidence: 99%

A Systematic Study on Zinc-Related Metabolism in Breast Cancer

Liu

Kong

et al. 2023

Nutrients

View full text Add to dashboard Cite

Breast cancer has become the most common cancer worldwide. Despite the major advances made in the past few decades in the treatment of breast cancer using a combination of chemotherapy, endocrine therapy, and immunotherapy, the genesis, treatment, recurrence, and metastasis of this disease continue to pose significant difficulties. New treatment approaches are therefore urgently required. Zinc is an important trace element that is involved in regulating various enzymatic, metabolic, and cellular processes in the human body. Several studies have shown that abnormal zinc homeostasis can lead to the onset and progression of various diseases, including breast cancer. This review highlights the role played by zinc transporters in pathogenesis, apoptosis, signal transduction, and potential clinical applications in breast cancer. Additionally, the translation of the clinical applications of zinc and associated molecules in breast cancer, as well as the recent developments in the zinc-related drug targets for breast cancer treatment, is discussed. These developments offer novel insights into understanding the concepts and approaches that could be used for the diagnosis and management of breast cancer.

show abstract

“…Moreover, due to the selective interaction with the folate receptors on MCF‐7 cells, this nanohybrid system reduced the IC 50 by 20–30 folds compared to free eugenol (Uma Maheswari et al, 2021). TQ incorporated zinc oxide NPs exhibited enhanced anticancer activity against MDA‐MB‐231 cells by inducing G2/M phase cell cycle arrest, DNA damage and apoptosis (Sjs et al, 2021). Zhang et al demonstrated that loading of curcumol into a pH responsive, metal–organic frameworks using porphyrin and Cu 2+ (CUR‐CuTPyP/F68) facilitated efficient accumulation and internalization in tumor cells.…”

Section: Nanoencapsulation Of Eos and Their Constituentsmentioning

confidence: 99%

Essential oils and their nanoformulations for breast cancer therapy

Thalappil,

Singh,

Carcereri de Prati

et al. 2023

Phytotherapy Research

View full text Add to dashboard Cite

Breast Cancer (BC) is the most prevalent type of cancer in the world. Current treatments include surgery, radiation, and chemotherapy but often are associated with high toxicity to normal tissues, chemoresistance, and relapse. Thus, developing novel therapies which could combat these limitations is essential for effective treatment. In this context, phytochemicals are increasingly getting popular due to their safety profile, ability to efficiently target tumors, and circumvent limitations of existing treatments. Essential Oils (EOs) are mixtures of various phytochemicals which have shown potential anticancer activity in preclinical BC models. However, their clinical translation is limited by factors such as high volatility, low stability, and poor solubility. Nanotechnology has facilitated their encapsulation in a variety of nanostructures and proven to overcome these limitations. In this review, we have efficiently summarized the current knowledge on the anticancer effect of EOs and constituents in both in in vitro and in in vivo BC models. Further, we also provide a descriptive account on the potential of nanotechnology in enhancing the anti‐BC activity of EOs and their constituents. The papers discussed in this review were selected using the keywords “antiproliferative Essential Oils in breast cancer,” “anticancer activity of Essential Oil in breast cancer,” and “cytotoxicity of Essential Oils in breast cancer” performed in PubMed and ScienceDirect databases.

show abstract

Modulation of gene expression by thymoquinone conjugated zinc oxide nanoparticles arrested cell cycle, DNA damage and increased apoptosis in triple negative breast cancer cell line MDA-MB-231

Cited by 6 publications

References 17 publications

Exploring the Potential of Thymoquinone-Stabilized Selenium Nanoparticles: In HEC1B Endometrial Cancer Cells Revealing Enhanced Anticancer Efficacy

Exploring the Potential of Thymoquinone-Stabilized Selenium Nanoparticles: In HEC1B Endometrial Cancer Cells Revealing Enhanced Anticancer Efficacy

A Systematic Study on Zinc-Related Metabolism in Breast Cancer

Essential oils and their nanoformulations for breast cancer therapy

Contact Info

Product

Resources

About