Modulation of fungal biofilm physiology and secondary product formation based on physico-chemical surface properties

Kakahi, Fatemeh Bajoul; Ly, Sarann; Tarayre, Cédric; Deschaume, Olivier; Bartic, Carmen; Wagner, Patrick; Compère, Philippe; Derdelinckx, Guy; Blecker, Christophe; Delvigne, Frank

doi:10.1007/s00449-019-02187-6

Cited by 13 publications

(7 citation statements)

References 53 publications

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“…For the wood pretreatment purpose, mixing the growing system continuously may stop the development of aerial hyphae, only biofilm hyphae would exist at the interfaces, and the adhesion could be improved. For the stand-alone adhesive purposes, surface mycelium could be produced at a large-scale in bioreactors with more control of the nutrient supply and air flow . As the mycelium composition and surface properties are highly influenced by the nutrient source, the attaching surface, and the growing environment, , it could be worthwhile to increase the active functional groups on the surface through controlling the substrate.…”

Section: Resultsmentioning

confidence: 99%

“…For the stand-alone adhesive purposes, surface mycelium could be produced at a large-scale in bioreactors with more control of the nutrient supply and air flow. 50 As the mycelium composition and surface properties are highly influenced by the nutrient source, the attaching surface, and the growing environment, 22,23 it could be worthwhile to increase the active functional groups on the surface through controlling the substrate. Alternatively, very limited fungal species (36 according to Elsacker et.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Functionality of Surface Mycelium Interfaces in Wood Bonding

Sun

Tajvidi

Howell

et al. 2020

ACS Appl. Mater. Interfaces

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Filamentous fungi have been considered as candidates to replace petroleum-based adhesives and plastics in novel composite material production, particularly those containing lignocellulosic materials. However, the nature of the role of surface mycelium in the adhesion between lignocellulosic composite components is not well-known. The current study investigated the functionality of surface mycelium for wood bonding by incubating Trametes versicolor on yellow birch veneers and compared the lap-shear strengths after hot-pressing to evaluate if the presence of surface mycelium can improve the interface between two wood layers and consequently improve bonding. We found that the lap-shear strength of the samples was enhanced by the increase of surface mycelium coverage up to 8 days of incubation (up to 1.74 MPa) without a significant wood weight loss. We provide evidence that the bottom surface of the mycelium layer is more hydrophilic, contains more small-scale filamentous structure and contains more functional groups, resulting in better bonding with wood than the top surface. These observations confirm and highlight the functionality of the surface mycelium layer for wood bonding and provide useful information for future developments in fully biobased composites manufacturing.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Functionality of Surface Mycelium Interfaces in Wood Bonding

Sun

Tajvidi

Howell

et al. 2020

ACS Appl. Mater. Interfaces

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“…Corrected mortality ratios were calculated to evaluate lethal concentrations [20] (Abbott, 1925) and were they linearized using logit transformation: log (P) = ln (P/1) -P) [23]. Linear regression was used to model the relationship between logit-transformed mortality and log-transformed values of fungal concentrations as an explanatory variable: logit (P) = slope × ln (concentration) + intercept.…”

Section: Discussionmentioning

confidence: 99%

“…This support was composed of two pieces of porous stainless steel 316 L (70 mm × 60 mm, Sulzer, Winterthur, Switzerland) that were arranged in opposition and attached ( Figure 1). The same system has been previously used for growing Trichoderma harzianum for the production of hydrophobin [20] and for the growth of Rhizopus oryzae in interaction with yeasts and lactic acid bacteria [21]. The cultures were performed at 30 • C and 125 rpm for five days.…”

Section: Aspergillus Cultivationmentioning

confidence: 99%

Biofilm Mode of Cultivation Leads to an Improvement of the Entomotoxic Patterns of Two Aspergillus Species

et al. 2020

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Two fungi, i.e., Aspergillus flavus Link and Aspergillus oryzae (Ahlb.) E. Cohn, were cultivated according to two methodologies, namely submerged and biofilm cultures with the primary aim to use their secondary metabolites the supernatant CL50, and CL90 varied between 1.3% (v/v) to 12.7% (v/v) for incubation times from 24 to 72 h. While the A. flavus supernatant entomotoxicity was higher than this of A. oryzae, the biofilm culture application increased the efficiency of the former. Proteomic analysis of the supernatants revealed discrepancies among the two species and modes of cultivation. Furthermore, the secondary metabolite profiles of both Aspergillus cultures were verified. Aspergillic acid, beta-cyclopiazonic acid, cyclopiazonic acid, ferrineospergillin, flavacol, and spermadin A were most predominant. Generally, these secondary metabolites were present in higher concentrations in the supernatants of A. flavus and biofilm cultures. These molecular identifications correlated positively with entomotoxic activity. Noteworthy, the absence of carcinogenic aflatoxins was remarkable, and it will allow further valorization to produce A. flavus to develop potential biopesticides.

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“…Bajoul Kakahi et al (2019) studied the effects of the physico-chemical properties of different materials, such as Teflon, glass, Viton™ rubber, silicon rubber, and stainless steel, on biofilm formation on their surfaces. A shake flask and a drip flow reactor were used to grow the biomasses and analyze the surface properties (e.g., hydrophobicity, surface energy, and roughness).…”

mentioning

confidence: 99%

Biological fixed‐film systems

Kim

Jae-Hoon

Choi

2020

Water Environment Research

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The technical papers published in 2019 regarding wastewater treatment and microbial films were classified into two categories: biofilm and biofilm reactors. The biofilm category includes biofilm formation, biofilm consortia, bacterial signals, biofouling, extracellular polymeric substances, and biofilm membrane bioreactors. The biofilm reactors category provides recent information on rotating biological contactors, fluidized-bed biofilm reactors, integrated fixed-film activated sludge, moving-bed biofilm reactors, packed-bed biofilm reactors, sequencing biofilm batch reactors, and trickling filters.

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Modulation of fungal biofilm physiology and secondary product formation based on physico-chemical surface properties

Cited by 13 publications

References 53 publications

Functionality of Surface Mycelium Interfaces in Wood Bonding

Functionality of Surface Mycelium Interfaces in Wood Bonding

Biofilm Mode of Cultivation Leads to an Improvement of the Entomotoxic Patterns of Two Aspergillus Species

Biological fixed‐film systems

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