Modulation of eosinophil function <i>in vitro</i>

Kay, A. B.

doi:10.1111/j.1365-2222.1990.tb02474.x

Cited by 8 publications

(3 citation statements)

References 30 publications

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“…However, MC activation is likely insufficient by itself (18). MC mediators histamine, prostaglandin D 2 (18), tumor necrosis factor (7), interleukin 5 (IL-5), (a potent eosinophil activator) (9), and leukotriene C 4 induce skin inflammation but not a typical LPR. Thus, the exact link between MC activation and subsequent LPR requires further investigation.…”

mentioning

confidence: 99%

Quantitative Analysis of T-Cell Receptor β Variable-Gene Usage in Cutaneous Late-Phase Reactions: Implications for T-Lymphocyte Recruitment in Cutaneous Inflammation

Lessin

Benoit²,

Li³

et al. 1999

Clin Diagn Lab Immunol

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To determine if functionally distinct T-lymphocyte (T cell) subsets accumulate in late-phase immunoglobulin E-mediated reactions (LPR), we quantitatively analyzed the immunophenotype and the T-cell receptor β variable-gene (Vβ) repertoire of T cells in cutaneous LPR. Peripheral blood and skin biopsies were obtained 6 or 24 h after sensitive subjects were challenged with intradermal injections of grass pollen allergen (Ag) and control (C) solution. The frequency of cells expressing CD3, CD4, CD8, CD45RO, and CD25/mm2 was determined by immunohistochemistry in nine subjects. Vβ usage was assessed by reverse transcription-PCR in five of nine subjects. A significantly greater frequency of CD3+ and CD45RO+ (memory) T cells was detected in Ag sites than in C sites at 24 h after challenge but not at 6 h. The frequency of activated (CD25+) and helper (CD4+) T cells appeared to be increased in Ag sites as well, though not significantly. Vβ6 was the most commonly expressed Vβ detected in Ag sites, but it was also detected in accompanying C sites. Vβ2 was the most commonly expressed Vβ detected in C sites. Sequence analysis in one case revealed Vβ expression in a 6-h Ag site to be essentially polyclonal. Our findings suggest that memory T cells with Vβ expression similar to that in normal skin accumulate in developing cutaneous LPR. The limited usage of Vβ suggests a preferential recruitment or retention of reactive T cells from an endogenous subset of skin-homing T cells with its own skewed Vβ repertoire.

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confidence: 99%

Quantitative Analysis of T-Cell Receptor β Variable-Gene Usage in Cutaneous Late-Phase Reactions: Implications for T-Lymphocyte Recruitment in Cutaneous Inflammation

Lessin

Benoit²,

Li³

et al. 1999

Clin Diagn Lab Immunol

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“…In the present studies, it is interesting to note that use of IFN‐γ was associated with increasing eosinophil counts but did not influence nasal hypersensitivity reactions. Thus, although eosinophils may play an important role in viral induced allergic inflammation [33, 34], eosinophils did not seem to contribute to nasal hypersensitivity to OVA in the current experimental setting. IFN‐γ is a classical Th1 cytokine that has been shown to reduce allergic reactions when administered during sensitization [35].…”

Section: Discussionmentioning

confidence: 89%

Influence of viral infection on the development of nasal hypersensitivity

Okamoto¹,

Matsuzaki²,

Matsuoka³

et al. 2005

Clin Experimental Allergy

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“…In vitro, this cytokine exerts specific functions on eosinophil cell lines. It enhances the proliferation of eosinophil precursors, the effector capacity and the life span of mature eosinophils [20][21][22][23], Moreover, mRNA of IL-5 has been recently found in tissue eosinophils from patients with celiac disease [7] and asthma [24], Eosino phils, thus, might regulate the immune reaction by the secretion of cytokines [25], Using ISH in a case of EC, we observed numerous pos itive cells throughout the lamina propria of bladder sec tions. IL-5 mRNA transcription seems to be enhanced in EC.…”

Section: Discussionmentioning

confidence: 99%