Modulation of dietary protein to lipid ratios for gilthead seabream on-growing during summer temperature conditions

Aragão, Cláudia; Cabano, Miguel; Colen, Rita; Teodósio, Rita; Gisbert, Enric; Dias, Jorge; Engrola, Sofía

doi:10.1016/j.aqrep.2022.101262

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…All diets were manufactured as described by Machado et al [ 34 ]. Dietary AA content was analyzed by ultra-high performance liquid chromatography (UPLC) after acid hydrolysis, as described in Aragão et al [ 35 ]. Tryptophan was determined by UPLC, after alkaline hydrolysis (6 M NaOH and methanesulfonic acid at 110 °C over 22 h in nitrogen-flushed glass vials).…”

Section: Methodsmentioning

confidence: 99%

Tryptophan Modulatory Role in European Seabass (Dicentrarchus labrax) Immune Response to Acute Inflammation under Stressful Conditions

Machado¹,

Peixoto

Santos³

et al. 2022

IJMS

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The present work aimed to study the role of dietary tryptophan supplementation in modulating the European seabass (Dicentrarchus labrax) immune condition during stressful rearing conditions (i.e., 15 days exposure to high density), as well as the immune response to acute inflammation after intraperitoneal injection of a bacterial pathogen. Stress alone did not compromise seabass health indicators. In contrast, a clear peripheral and local inflammatory response was observed in response to the inoculated bacteria. Moreover, exposure to a high stocking density seemed to exacerbate the inflammatory response at early sampling points, compared to fish stocked at a lower density. In contrast, stressed fish presented some immune-suppressing effects on the T-cell surface glycoprotein receptor expressions at a late sampling point following inflammation. Regarding the effects of dietary tryptophan, no changes were observed on seabass immune indicators prior to inflammation, while a small number of immunosuppressive effects were observed in response to inflammation, supporting tryptophan’s role in the promotion of immune-tolerance signals during inflammation. Nonetheless, tryptophan dietary supplementation improved the inflammatory response against a bacterial pathogen during stressful conditions, supported by a reduction of plasma cortisol levels, an up-regulation of several immune-related genes at 48 h, and an inversion of the previously observed, stress-induced T-cell suppression. Finally, the involvement of tryptophan catabolism in macrophages was confirmed by the up-regulation of genes involved in the kynurenine pathway. The present study brings new insights regarding the immune modulatory role of tryptophan during stressful conditions in fish, thus allowing for the development of novel prophylactic protocols during vaccination by intraperitoneal injection in the European seabass.

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Section: Methodsmentioning

confidence: 99%

Tryptophan Modulatory Role in European Seabass (Dicentrarchus labrax) Immune Response to Acute Inflammation under Stressful Conditions

Machado¹,

Peixoto

Santos³

et al. 2022

IJMS

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“…Taurine content was analysed in freeze-dried-diet, fishbody, and liver samples. Amino acid and taurine analyses were performed according to the procedures described in Aragão [52].…”

Section: Chemical and Biochemical Analysismentioning

confidence: 99%

Taurine Supplementation to Plant-Based Diets Improves Lipid Metabolism in Senegalese Sole

Aragão

Teodósio²,

Colen³

et al. 2023

Animals

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Taurine is a sulphur-containing amino acid with important physiological roles and a key compound for the synthesis of bile salts, which are essential for the emulsion and absorption of dietary lipids. This study aimed to evaluate the effects of taurine supplementation to low-fishmeal diets on the metabolism of taurine, bile acids, and lipids of Senegalese sole. A fishmeal (FM) and a plant-protein-based (PP0) diet were formulated, and the latter was supplemented with taurine at 0.5 and 1.5% (diets PP0.5 and PP1.5). Diets were assigned to triplicate tanks containing 35 fish (initial weight ~14 g) for 6 weeks. Fish from the PP0 treatment presented lower taurine and bile-acid concentrations compared with the FM treatment, and a downregulation of cyp7a1 and abcb11 was observed. Triolein catabolism decreased in PP0-fed fish, resulting in increased hepatic fat content and plasma triglycerides, while no effects on plasma cholesterol were observed. Taurine supplementation to plant-based diets resulted in a higher taurine accumulation in fish tissues, increased bile-acid concentration, and upregulation of cyp7a1 and abcb11. Hepatic fat content and plasma triglycerides decreased with increasing dietary taurine supplementation. Taurine supplementation mitigated part of the negative effects of plant-based diets, leading to better lipid utilisation.

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Discarded blue mussel ( Mytilus edulis ): a feed ingredient that maintains growth performance of juvenile gilthead seabream ( Sparus aurata ) while fishmeal and fish oil are removed

Jaeger,

Corraze,

Gayet

et al. 2024

Journal of Applied Aquaculture

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Modulation of dietary protein to lipid ratios for gilthead seabream on-growing during summer temperature conditions

Cited by 3 publications

References 37 publications

Tryptophan Modulatory Role in European Seabass (Dicentrarchus labrax) Immune Response to Acute Inflammation under Stressful Conditions

Tryptophan Modulatory Role in European Seabass (Dicentrarchus labrax) Immune Response to Acute Inflammation under Stressful Conditions

Taurine Supplementation to Plant-Based Diets Improves Lipid Metabolism in Senegalese Sole

Discarded blue mussel ( Mytilus edulis ): a feed ingredient that maintains growth performance of juvenile gilthead seabream ( Sparus aurata ) while fishmeal and fish oil are removed

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