Modulation of D1 protein turnover under cadmium and heat stresses monitored by [35S]methionine incorporation

Franco, Emanuel; Alessandrelli, S.; Masojídek, Jiří; Margonelli, Andrea; Giardi, Maria Teresa

doi:10.1016/s0168-9452(99)00040-0

Cited by 45 publications

(25 citation statements)

References 30 publications

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“…4a, b). The alteration of the D1 and oxygen-evolving complex proteins appeared to be similar to those found when Pisum sativum and Zea mays were exposed to cadmium and copper, respectively (Baron et al 1995;Franco et al 1999). With no doubt, we showed for Cr to affect the turnover of PSII D1 protein and to diminish the content of 24 and 33 kDa proteins of the OEC, which may contribute to decrease the PSII electron transport as we also indicated here.…”

Section: Resultssupporting

confidence: 77%

“…Inhibition of Zea mays photosynthesis induced by copper was found to be caused by the alteration of proteins having a role in the oxygen-evolving complex (Baró n et al 1995). Cadmium inhibition of PSII activity in Pisum sativum leaves was shown to be caused by the alteration of D1 protein indicating for the inhibitory site to be on the reducing side of PSII (Franco et al 1999).…”

Section: Introductionmentioning

confidence: 97%

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Inhibition of photosystem II photochemistry by Cr is caused by the alteration of both D1 protein and oxygen evolving complex

et al. 2006

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The effect of chromium (Cr) on photosystem II (PSII) electron transport and the change of proteins content within PSII complex were investigated. When Lemna gibba was exposed to Cr during 96 h, growth inhibition was found to be associated with an alteration of the PSII electron transport at both PSII oxidizing and reducing sides. Investigation of fluorescence yields at transients K, J, I, and P suggested for Cr inhibitory effect to be located at the oxygen-evolving complex and Q(A) reduction. Those Cr-inhibitory effects were related to the change of the turnover of PSII D1 protein and the alteration of 24 and 33 kDa proteins of the oxygen-evolving complex. The inhibition of the PSII electron transport and the formation of reactive oxygen species induced by Cr were highly correlated with the decrease in the content of D1 protein and the amount of 24 and 33 kDa proteins. Therefore, functional alteration of PSII activity by Cr was closely related with the structural change within PSII complex.

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Section: Resultssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 97%

Inhibition of photosystem II photochemistry by Cr is caused by the alteration of both D1 protein and oxygen evolving complex

et al. 2006

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“…Some studies have also described increased D1 turnover as a physiological stress-compensating mechanism. Increased D1 turnover occurs in response to fumigation with ozone (36) or with mixtures of air pollutants (O 3 , NO 2 , and SO 2 ) (58), as well as during potassium deficiency (58), cadmium exposure (30,34), and drought (35). It has been proposed that D1 turnover acts as a general adaptive response to environmental extremes (35), which gives further support to our hypothesis of increased D1 turnover as an Irgarol tolerance mechanism.…”

Section: Vol 75 2009 Irgarol Tolerance and Psba Genes In Periphytonsupporting

confidence: 69%

Community-Level Analysis of psbA Gene Sequences and Irgarol Tolerance in Marine Periphyton

Eriksson

Clarke

Franzén

et al. 2009

Appl Environ Microbiol

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This study analyzes psbA gene sequences, predicted D1 protein sequences, species relative abundance, and pollution-induced community tolerance in marine periphyton communities exposed to the antifouling compound Irgarol 1051. The mechanism of action of Irgarol is the inhibition of photosynthetic electron transport at photosystem II by binding to the D1 protein. The metagenome of the communities was used to produce clone libraries containing fragments of the psbA gene encoding the D1 protein. Community tolerance was quantified with a short-term test for the inhibition of photosynthesis. The communities were established in a continuous flow of natural seawater through microcosms with or without added Irgarol. The selection pressure from Irgarol resulted in an altered species composition and an inducted community tolerance to Irgarol. Moreover, there was a very high diversity in the psbA gene sequences in the periphyton, and the composition of psbA and D1 fragments within the communities was dramatically altered by increased Irgarol exposure. Even though tolerance to this type of compound in land plants often depends on a single amino acid substitution (Ser 264 3Gly) in the D1 protein, this was not the case for marine periphyton species. Instead, the tolerance mechanism likely involves increased degradation of D1. When we compared sequences from low and high Irgarol exposure, differences in nonconserved amino acids were found only in the so-called PEST region of D1, which is involved in regulating its degradation. Our results suggest that environmental contamination with Irgarol has led to selection for high-turnover D1 proteins in marine periphyton communities at the west coast of Sweden.Development of tolerance, or resistance, to anthropogenic toxicants released into the environment is an issue of increasing importance. Reports of tolerant organisms are increasing, and tolerance toward a variety of compounds has been found (22,44,46,100). This is essentially evolution in action and shows that toxicants can act as selective pressures in the environment. Even though high concentrations of toxicants can be released in episodes or pulses, the overall environmental concentrations of toxicants are often quite low, which implies that adaptation and nonlethal selection are more common than acute and/or lethal effects.Since sensitivities to a given toxicant differ within species and even more so between species (12), a toxicant-induced succession (TIS) will occur in toxicant-exposed communities, where sensitive species, individuals, or genotypes are replaced by more tolerant ones, giving an increase in average tolerance in the community. This chain of events is fundamental for the pollution-induced community tolerance (PICT) concept (14). PICT studies can be performed in natural or model ecosystems and have the important advantage of a causal link between exposure and effect. The concept has been used to demonstrate long-term selection pressure from toxicants on several types of communities, as reviewed by Blanck (10) and...

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“…A 2-fold increase in D1 protein after 5 h of Cd exposure was observed in cut petioles of Vicia faba seedlings by pulse labeling with [ 35 S]methionine. This increase, however, was short lived, and D1 subsequently was found to decrease upon longer ( ‡24 h) Cd exposure times (Franco et al 1999).…”

Section: Psbamentioning

confidence: 97%

Cadmium- and iron-stress-inducible gene expression in the green alga Chlamydomonas reinhardtii : evidence for H43 protein function in iron assimilation

Rubinelli¹,

Siripornadulsil

Gao-Rubinelli

et al. 2002

Planta

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Early transcriptional responses of a cell wall-deficient mutant of the green alga Chlamydomonas reinhardtii to heavy-metal stress have been investigated using the method of mRNA differential display. We have identified, sequenced, and quantified the induction of a number of transcripts that are up-regulated by a brief (2-h) exposure to 25 microm cadmium chloride, including one transcript which is also highly responsive to iron (Fe) deficiency. These transcripts represent both nuclear- and chloroplast-encoded genes, and include both novel genes and genes with known or suspected functions. Among these is a gene with significant homology to HCR1, a high-CO(2)- and Fe-deficiency-inducible gene from Chlorococcum littorale. We further characterized the regulation of the HCR1-like gene ( H43) and found that this transcript is also induced by Fe-depletion of the medium. Heterologous expression of H43 in the Fe-uptake mutant fet3fet4 of Saccharomyces cerevisiae resulted in partial suppression of the slow-growth phenotype of this mutant in minimal medium, and resulted in a 2-fold increase in Fe accumulation per cell. Our results demonstrate the utility of Chlamydomonas cw(-) strains for functional genomics studies of metal stress. The magnitudes of induction and functional analyses suggest possible utility for these genes in the study of metal stress sensing in green plants and development of novel Fe acquisition and phytoremediation strategies.

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Modulation of D1 protein turnover under cadmium and heat stresses monitored by [35S]methionine incorporation

Cited by 45 publications

References 30 publications

Inhibition of photosystem II photochemistry by Cr is caused by the alteration of both D1 protein and oxygen evolving complex

Inhibition of photosystem II photochemistry by Cr is caused by the alteration of both D1 protein and oxygen evolving complex

Community-Level Analysis of psbA Gene Sequences and Irgarol Tolerance in Marine Periphyton

Cadmium- and iron-stress-inducible gene expression in the green alga Chlamydomonas reinhardtii : evidence for H43 protein function in iron assimilation

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