Modulation of cyclic CO2 release in response to endogenous changes of metabolism during pupal development of Zophobas rugipes (Coleoptera: Tenebrionidae)

Kaiser, Alexander; Hartzendorf, Sandra; Wobschall, A.; Hetz, Stefan K.

doi:10.1016/j.jinsphys.2009.06.010

Cited by 12 publications

(8 citation statements)

References 66 publications

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“…Recently, Kaiser et al showed that increases in metabolic rate, due to endogenous factors related to developmental stages, also cause the C phase of the DGC to shorten (Kaiser et al, 2010). In this study we found no evidence that humidity affected respiratory pattern.…”

Section: Discussioncontrasting

confidence: 80%

The effect of ambient humidity and metabolic rate on the gas-exchange pattern of the semi-aquatic insectAquarius remigis

Contreras

Bradley

2011

Journal of Experimental Biology

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SUMMARYWe have examined the effects of temperature on metabolic rate and respiratory pattern in the water strider Aquarius remigis. As temperature was increased from 10 to 30°C, the metabolic rate of the insects increased and the respiratory pattern transitioned from discontinuous, to cyclic, to continuous. The discontinuous gas-exchange cycle (DGC) was observed even in insects standing on water when the respirometry chamber was being perfused with humid (>95% relative humidity) air. Comparisons of insects at 20°C in humid and dry air showed no statistically significant differences in metabolic rate or respiratory pattern (P>0.05). The proportion of time that the spiracles were closed was greater at 10°C than at 20°C (P<0.01), and greater at 20°C than at 30°C (P<0.05). These results are compatible with the hypothesis that the respiratory patterns of insects are determined by the relationship between oxygen supply and oxygen demand. There was no evidence in this insect that humidity had any effect on the respiratory pattern. The results are discussed in the context of the ongoing discussion in the literature of the origin, maintenance and adaptive significance of the DGC in insects.

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Section: Discussioncontrasting

confidence: 80%

The effect of ambient humidity and metabolic rate on the gas-exchange pattern of the semi-aquatic insectAquarius remigis

Contreras

Bradley

2011

Journal of Experimental Biology

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“…As the pupa is a relatively stable and immobile stage, the larval stage is the critical stage to obtain energy to complete the metamorphosis [ 33 ], and the energy derived from larvae can also make a significant contribution to the reproductive output during their entire life [ 34 , 35 ]. Therefore, many previous studies have reported that energy metabolic rates declined sharply after pupariation, and are maintained at a low level until the eclosion to the adult [ 36 , 37 ]. Similarly, the present KEGG analysis also confirmed the previous study in D. melanogaster [ 38 ], that the oxidative phosphorylation pathway was significantly blocked, indicating less production of energy in LWS and WPS, when comparing to WS in B. dorsalis .…”

Section: Discussionmentioning

confidence: 99%

RNA-seq analysis of gene expression changes during pupariation in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

et al. 2018

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BackgroundThe oriental fruit fly, Bactrocera dorsalis (Hendel) has been considered to be one of the most important agricultural pest around the world. As a holometabolous insect, larvae must go through a metamorphosis process with dramatic morphological and structural changes to complete their development. To better understand the molecular mechanisms of these changes, RNA-seq of B. dorsalis from wandering stage (WS), late wandering stage (LWS) and white puparium stage (WPS) were performed.ResultsIn total, 11,721 transcripts were obtained, out of which 1914 genes (578 up-regulated and 1336 down-regulated) and 2047 genes (655 up-regulated and 1392 down-regulated) were found to be differentially expressed between WS and LWS, as well as between WS and WPS, respectively. Of these DEGs, 1862 and 1996 genes were successfully annotated in various databases. The analysis of RNA-seq data together with qRT-PCR validation indicated that during this transition, the genes in the oxidative phosphorylation pathway, and genes encoding P450s, serine protease inhibitor, and cuticular proteins were down-regulated, while the serine protease genes were up-regulated. Moreover, we found some 20-hydroxyecdysone (20E) biosynthesis and signaling pathway genes had a higher expression in the WS, while the genes responsible for juvenile hormone (JH) synthesis, degradation, signaling and transporter pathways were down-regulated, suggesting these genes might be involved in the process of larval pupariation in B. dorsalis. For the chitinolytic enzymes, the genes encoding chitinases (chitinase 2, chitinase 5, chitinase 8, and chitinase 10) and chitin deacetylase might play the crucial role in the degradation of insect chitin with their expressions significantly increased during the transition. Here, we also found that chitin synthase 1A might be involved in the chitin synthesis of cuticles during the metamorphosis in B. dorsalis.ConclusionsSignificant changes at transcriptional level were identified during the larval pupariation of B. dorsalis. Importantly, we also obtained a vast quantity of RNA-seq data and identified metamorphosis associated genes, which would all help us to better understand the molecular mechanism of metamorphosis process in B. dorsalis.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5077-z) contains supplementary material, which is available to authorized users.

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“…For species which show discontinuous and/or cyclic gas exchange (see Marais et al, 2005;Hetz, 2007;Contreras and Bradley, 2009;Kaiser et al, 2010), several generalities regarding the acute effects of temperature now exist. For example, in some species the pattern of gas exchange shifts from predominantly discontinuous to cyclic or continuous as trial temperature increases (e.g.…”

Section: Introductionmentioning

confidence: 99%

Phenotypic plasticity of gas exchange pattern and water loss in Scarabaeus spretus (Coleoptera: Scarabaeidae): deconstructing the basis for metabolic rate variation

Terblanche

Clusella‐Trullas

Chown

2010

Journal of Experimental Biology

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SUMMARYInvestigation of gas exchange patterns and modulation of metabolism provide insight into metabolic control systems and evolution in diverse terrestrial environments. Variation in metabolic rate in response to environmental conditions has been explained largely in the context of two contrasting hypotheses, namely metabolic depression in response to stressful or resource-(e.g. water) limited conditions, or elevation of metabolism at low temperatures to sustain life in extreme conditions. To deconstruct the basis for metabolic rate changes in response to temperature variation, here we undertake a full factorial study investigating the longer-and short-term effects of temperature exposure on gas exchange patterns. We examined responses of traits of gas exchange [standard metabolic rate (SMR); discontinuous gas exchange (DGE) cycle frequency; cuticular, respiratory and total water loss rate ( -1 in 15°C-acclimated beetles to 3.1±0.5mgh -1 in 25°C-acclimated beetles measured at 25°C). Respiratory WLR was reduced from 2.25±0.40mgh -1 in 15°C-acclimated beetles to 1.60±0.40mgh -1 in 25°C-acclimated beetles measured at 25°C, suggesting conservation of water during DGE bursts. Overall, this suggests water conservation is a priority for S. spretus exposed to longer-term temperature variation, rather than elevation of SMR in response to low temperature acclimation, as might be expected from a beetle living in a relatively warm, low rainfall summer region. These results are significant for understanding the evolution of gas exchange patterns and trade-offs between metabolic rate and water balance in insects and other terrestrial arthropods.

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Modulation of cyclic CO2 release in response to endogenous changes of metabolism during pupal development of Zophobas rugipes (Coleoptera: Tenebrionidae)

Cited by 12 publications

References 66 publications

The effect of ambient humidity and metabolic rate on the gas-exchange pattern of the semi-aquatic insectAquarius remigis

The effect of ambient humidity and metabolic rate on the gas-exchange pattern of the semi-aquatic insectAquarius remigis

RNA-seq analysis of gene expression changes during pupariation in Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

Phenotypic plasticity of gas exchange pattern and water loss in Scarabaeus spretus (Coleoptera: Scarabaeidae): deconstructing the basis for metabolic rate variation

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