Modulation of Corneal Epithelial Cell Functions by the Neutrophil-Derived Inflammatory Mediator CAP37

Abstract

CAP37 modulated corneal epithelial cell proliferation and migration and upregulated adhesion molecules involved in leukocyte-epithelial and epithelial-extracellular matrix interactions.

Lymphocyte Function-Associated Antigen-1-Dependent Inhibition of Corneal Wound Healing

“…Exocytosis of these classes of neutrophil granules occurs rapidly after activation by chemokines 51 shown to be present in wounded corneas within the time frame of leukocyte accumulation. 52 Another neutrophil-derived factor of potential importance is CAP37, which was shown by Pereira and colleagues 53 to augment corneal epithelial cell migration and proliferation. Thus, it is possible that the leukocyte functions extend beyond scavenging.…”

CAP37 Activation of PKC Promotes Human Corneal Epithelial Cell Chemotaxis

“…A monospecific, rabbit antiserum shown to be specific for CAP37 has been previously described. 13 Cell Culture SV-40 adenovirus immortalized HCECs were a gift from James Chodosh (Boston, MA) and were maintained as previously described 5,19 in defined keratinocyte-serum free media (keratinocyte serum-free media [SFM]; Gibco, Grand Island, NY) containing L-glutamine (2 mM; Gibco); antibiotic-antimycotic (0.1 units/mL penicillin G sodium, 100 lg/mL streptomycin sulfate, 0.25 lg/mL amphotericin B; Gibco); and growth FIGURE 1. Chemotaxis of HCECs in response to CAP37 is mediated by PKC signaling through a G protein-coupled receptor.…”

“…3,4 CAP37, known initially for its antimicrobial activity, is now recognized to have a number of novel and important effects on mammalian cells. [3][4][5][6] Prior findings from our laboratory indicate that CAP37 plays a role in host defense and inflammation. [5][6][7][8] CAP37 regulates monocyte, macrophage, and microglial functions by promoting migration, phagocytosis, and activation of these cells to produce proinflammatory cytokines.…”

“…[3][4][5][6] Prior findings from our laboratory indicate that CAP37 plays a role in host defense and inflammation. [5][6][7][8] CAP37 regulates monocyte, macrophage, and microglial functions by promoting migration, phagocytosis, and activation of these cells to produce proinflammatory cytokines. 3,9,10 Furthermore, CAP37 upregulates adhesion molecules on endothelial, smooth muscle, and corneal epithelial cells.…”

“…Its induced expression in corneal epithelial cells in response to infection suggests a role in host defense and inflammation. 5,12 The role of endogenously induced CAP37 in facilitating the healing of corneal wounds remains unknown and is the focus of future studies.…”