Modulation of Chitotriosidase During Macrophage Differentiation

Rosa, Michelino Di; Malaguarnera, Giulia; Gregorio, Corinne De; D’Amico, Fabio; Mazzarino, María Clorinda; Malaguarnera, Mariano

doi:10.1007/s12013-012-9471-x

Cited by 51 publications

(37 citation statements)

References 35 publications

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“…On the fifth day levels of CHIT-1 expression were increased by 15.12, and on the seventh day, the expression of CHIT-1 reached an increase by 15.75 fold. In a previous study quantitative evaluation of CHIT-1 protein confirmed that CHIT-1 enhancement is a mechanism operant with macrophages differentiation and polarization [35]. In contrast, YKL-39 expression was unchanged in the diverse stage of HMMs differentiation.…”

Section: Expression Of Ykl-39 and Chit-1 During In Vitro Differentiatmentioning

confidence: 75%

Comparison of YKL-39 and CHIT-1 expression during macrophages differentiation and polarization

Rosa¹,

Tibullo²,

Malaguarnera³

et al. 2013

MRI

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The chitinase-like proteins YKL-39 (chitinase 3-like-2) and Chitortriosidase (CHIT-1) are members of the chitinases family. YKL-39 expression has been associated with osteoarthritis, whereas CHIT-1 activity is regarded as a biochemical marker of macrophage activation. So far, the physiological or pathological role of YKL-39 in the inflammation is still poorly understood. We compared YKL-39 and CHIT-1 modulation during monocyte to macrophage transition and polarization. Gene expression analysis was investigated by real-time PCR from mRNA of human monocytes obtained from buffy coat of healthy volunteers, from mRNA of polarized macrophages to classically activated macrophages (or M 1 ), obtained by interferon-γ and lipopolysaccharide exposure, and from mRNA of alternatively activated macrophages (or M 2 ) obtained by interleukin-4 exposure. We demonstrated different variations of YKL-39 and CHIT-1 production during macrophages polarization. CHIT-1 levels gradually increase in the course of the time with a peak of expression between the fifth and the seventh day of culture. In contrast, YKL-39 expression was unaltered in the diverse stage of HMMs differentiation, but increased significantly in M 1 polarized macrophages and reverted to base levels in M 2 polarized macrophages. These findings indicated that the function of YKL-39 is much more restricted and selective than that exerted by CHIT-1.

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Section: Expression Of Ykl-39 and Chit-1 During In Vitro Differentiatmentioning

confidence: 75%

Comparison of YKL-39 and CHIT-1 expression during macrophages differentiation and polarization

Rosa¹,

Tibullo²,

Malaguarnera³

et al. 2013

MRI

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“…Conversely, during the development of acute/chronic inflammatory disorders, the enzymatic activity of CHIT1 increases significantly [8]. During the effective maturation of monocytes into macrophages, CHIT1 levels increased significantly during the diverse stages of macrophage maturation and was modulated in polarized M1 and M2 macrophages, indicating a different role of this enzyme in the specialized macrophages [11] and underscoring that CHIT1 can be regarded not only as a marker of macrophage activation but also as an important regulator of inflammatory functions. …”

Section: Chit1 and Dmmentioning

confidence: 99%

“…The phenotypes of M1/M2 macrophages can be exchanged depending on the conditions of their microenvironment [31]. Noteworthy, CHIT1 levels increased significantly during the diverse stages of macrophage maturation, and were modulated in polarized M1 and M2 macrophages, indicating a different role of this enzyme in the specialized macrophages [11]. …”

Section: Chit1 and Dmmentioning

confidence: 99%

“…It has been reported that IL-4 promotes a M2 phenotype in regressive atherosclerotic lesions [34], providing in such a way a potential explanation of CHIT1 activity associated with the presence of atherosclerosis [12]. Paradoxically, it was found that in monocytes IL-4 treatment induced a significant increase on mRNA CHIT1 expression [11]. IL-4 is a well-appreciated antagonist of the M1 response and macrophage proinflammatory properties [35].…”

Section: Chit1 and Dmmentioning

confidence: 99%

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Chitotriosidase: A New Inflammatory Marker in Diabetic Complications

Rosa

Malaguarnera²

2016

Pathobiology

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Chitotriosidase (CHIT1) belongs to chitinase family. So far this enzyme has been the best investigated human chitinase regarding its biological activity and association with various disorders. In a healthy population, CHIT1 activity is very low and originates in the circulating polymorphonuclear cells. Conversely, during the development of acute/chronic inflammatory disorders, the enzymatic activity of CHIT1 increases significantly. Recently, CHIT1 has also been involved in the pathogenesis of diabetes mellitus (DM). Mounting evidence from experimental studies revealing the increase of CHIT1 levels in pathological conditions, such as atherosclerosis, coronary artery disease, acute ischemic stroke, cerebrovascular dementia, nonalcoholic fatty liver disease, and osteolytic processes suggest its critical role in the evolutions and complications of DM. This review is addressed to provide mechanistic insights by highlighting the relationship between CHIT1 and diabetes, and their contribution in the exacerbation of this disease.

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“…While stimulation by TLR ligand/ IFN-γ leads to classical M1 activation, stimulation by IL-4/IL-1 leads to M2 activation [22]. It has been reported that IL-4 treatment induced the expression of mRNA CHIT1 and activated PPAR-γ [23,24]. IL-4 is also an antagonist of the M1 response.…”

Section: Discussionmentioning

confidence: 99%

Effect of PPAR-γ agonists on macrophage activation in type2 diabetes mellitus

Sözmen¹,

Karakaş²,

Topçugil³

et al. 2018

Ann Clin Anal Med

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Effect of (PPAR-γ) agonists on macrophage activationEffect of PPAR-γ agonists on macrophage activation in type2 diabetes mellitus DOI: 10.4328 AbstractAim: Recently, it has been proposed that inflammation triggered by macrophages as the pathogenic mechanism linked to the development of obesity-related insulin resistance. Peroxisome proliferator-activated receptor-γ (PPAR-γ) agonists which increase the insulin receptor sensitivity, might improve glycemic control by enhancing insulin sensitivity and ameliorate the impaired lipid profile. In this study, we assessed the effect of rosiglitazone (PPAR-γ agonist) on the insulin resistance and inflammatory markers. Material and Method: Thirty patients (11 men, 19 women) with type II diabetes mellitus (DM) were taken into the study. They were treated by rosiglitazone in a dose of 4mg/day as well as a diet for 12 weeks. Results: Rosiglitazone treatment significantly decreased HbA1c (p<0,01), IR-HOMA, hsCRP (p<0,01), triglyceride levels (p<0,05), CETP activity (p<0,01) and basal serum oxidation (TBARS levels, p<0,05). However, Chitotriosidase activity significantly increased after Rosiglitazone treatment (p<0,01). TNF, IL-6, sTNFR1, and sTNFR2 levels showed no statistically significant difference compared to their basal levels. Discussion: Rosiglitazone might treat diabetes by mediating glucose/lipid metabolism and preventing lipid oxidation in patients with DM. On the other hand, it has a dual role on inflammation; while it might induce macrophage activation suggesting its pro-inflammatory effect, it might also reduce the CRP levels by lowering gene expression suggesting its anti-inflammatory effect.

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Modulation of Chitotriosidase During Macrophage Differentiation

Cited by 51 publications

References 35 publications

Comparison of YKL-39 and CHIT-1 expression during macrophages differentiation and polarization

Comparison of YKL-39 and CHIT-1 expression during macrophages differentiation and polarization

Chitotriosidase: A New Inflammatory Marker in Diabetic Complications

Effect of PPAR-γ agonists on macrophage activation in type2 diabetes mellitus

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