Modulation of Cellular Response to Different Parameters of the Rotating Magnetic Field (RMF)—An In Vitro Wound Healing Study

Abstract

Since the effect of MFs (magnetic fields) on various biological systems has been studied, different results have been obtained from an insignificant effect of weak MFs on the disruption of the circadian clock system. On the other hand, magnetic fields, electromagnetic fields, or electric fields are used in medicine. The presented study was conducted to determine whether a low-frequency RMF (rotating magnetic field) with different field parameters could evoke the cellular response in vitro and is possible to modulate the cellular response. The cellular metabolic activity, ROS and Ca2+ concentration levels, wound healing assay, and gene expression analyses were conducted to evaluate the effect of RMF. It was shown that different values of magnetic induction (B) and frequency (f) of RMF evoke a different response of cells, e.g., increase in the general metabolic activity may be associated with the increasing of ROS levels. The lower intracellular Ca2+ concentration (for 50 Hz) evoked the inability of cells to wound closure. It can be stated that the subtle balance in the ROS level is crucial in the wound for the effective healing process, and it is possible to modulate the cellular response to the RMF in the context of an in vitro wound healing.

An Evaluation of the Usability of Argon Plasma-Treated Bacterial Cellulose as a Carrier for Controlled Releases of Glycoside Hydrolases PelAh and PslGh, Which Are Able to Eradicate Biofilm

“…Secondly, twenty-four hours after cell seeding, 6 different final concentrations (5.0, 10.0, 25.0, 50.0, 100.0, 250.0 µg/mL; 5 replicates of each dose) of the enzyme were prepared in DMEM medium and added to the cells. The effect of the recombinant enzyme on cell viability was calculated using Equation (2), as described elsewhere [35]:…”

“…The morphologies of the L929 cell line exposed to the recombinant enzyme at different concentrations and the control samples were analyzed using a Nikon TS-100 phase-contrast inverted microscope (NIS Elements F Package, camera Nikon DS-Fi1, Nikon, Melville, NY, USA) at 100× magnification [35].…”

The Effect of a Rotating Magnetic Field on the Regenerative Potential of Platelets

“…There are static fields (SMF) and dynamic fields, which include pulsed magnetic fields (PMF), rotating magnetic fields (RMF), and sinusoidal magnetic fields. In terms of frequency, electromagnetic fields can be divided into very low (<300 Hz), low, medium, and high (30 kHz-30 MHz) [9][10][11].…”

“…A rotating magnetic field can also positively alter the activity of laccase, making it a promising tool for enhancing enzyme properties [13]. RMF exposure can increase resistance to heat stress, reduce levels of ROS, affect intracellular calcium ion concentrations, and contribute to cell aging deceleration [9,10].…”

“…The samples were exposed to a rotating magnetic field for 1 h and 3 h at frequencies of 25 and 50 Hz at a maximum induction of Bmax = 42.64 mT. Previous studies evaluating the biological effects of RMF have indicated that the listed RMF parameters produce the most effective results in modifying cellular metabolization activity, intracellular calcium ions, and ROS concentrations [9,13,33]. We deliberately used EDTA plasma instead of PRP to see if this easier-to-obtain material could become an alternative to PRP.…”