Modulation of bone marrow mesenchymal stem cell secretome by ECM-like hydrogels

Silva, Nuno; Moreira, Joana S.; Ribeiro-Samy, Silvina; Gomes, Eduardo D.; Tam, Roger Y.; Shoichet, Molly S.; Reis, Rui L.; Sousa, Nuno; Salgado, António J.

doi:10.1016/j.biochi.2013.08.016

Cited by 52 publications

(49 citation statements)

References 28 publications

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“…Human fibroblasts 28 and adipose derived stem cellspeptide-modified alginate gels. More recently, furan functionalisation of GG, followed by a Diels-Alder reaction with a synthetic variant of the GRDGS peptide was used to direct neural and mesenchymal stem cell differentiation with encouraging results 30,31 . In this paper, we covalently linked unmodified peptides to GG, and monitored the reaction efficiency by tyrosine radiolabelling.…”

Section: Introductionmentioning

confidence: 99%

Peptide modification of purified gellan gum

et al. 2015

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Gellan gum (GG) is an anionic polysaccharide with potential as a biopolymer for additive manufacturing (3D-bioprinting) and tissue engineering. Previous studies have shown GG to be highly cytocompatible, but lacking specific attachment sites required for anchorage-dependent cells. In this work, we modify purified-GG polymer with a short peptide containing the arginine-glycine-aspartic acid (RGD) sequence that is known to enhance integrin-mediated cell attachment. Radiolabelling of the peptide was used in optimisation of the conjugation procedure to achieve an overall efficiency of 40%. The purification of divalent cations from commercial GG samples was found to be critical for successful conjugation. Rheological studies revealed that the peptide coupling did not prevent gelation behaviour. C2C12 cells showed improved attachment on the surface of and encapsulated within RGD-GG hydrogels, differentiating to multinucleated myofibers after 5-7 days. PC12 cells showed minimal interactions with both GG and RGD-GG, with formation of cell clusters and impedance of terminal differentiation and neurite extension. Gellan gum (GG) is an anionic polysaccharide with potential as a biopolymer for additive manufacturing (3D-bioprinting) and tissue engineering. Previous studies have shown GG to be highly cytocompatible, but lacking specific attachment sites required for anchorage-dependent cells. In this work, we modify purified-GG polymer with a short peptide containing the arginineglycine-aspartic acid (RGD) sequence that is known to enhance integrin-mediated cell attachment. Radiolabelling of the peptide was used in optimisation of the conjugation procedure to achieve an overall efficiency of 40%. The purification of divalent cations from commercial GG samples was found to be critical for successful conjugation. Rheological studies revealed that the peptide coupling did not prevent gelation behaviour. C2C12 cells showed improved attachment on the surface of and encapsulated within RGD-GG hydrogels, differentiating to multinucleated myofibers after 5-7 days. PC12 cells showed minimal interactions with both GG and RGD-GG, with formation of cell clusters and impedance of terminal differentiation and neurite extension.

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Section: Introductionmentioning

confidence: 99%

Peptide modification of purified gellan gum

et al. 2015

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“…In this sense, our group has modified GG with GRGDS fibronectin peptide, which resulted in the enhancement of cell proliferation and metabolic activity, as will be described in detail in the next section [130, 190]. …”

Section: Biomaterials As a Tissue Engineering Approach For Sci Repairmentioning

confidence: 99%

“…Four weeks after transplantation, increased cell survival as well as migration throughout the hydrogel was observed, accompanied by functional improvement of the animal, in comparison to animals that received just BM-MSCs or a vehicle control of PBS [240]. More recently, GG was also suggested for MSCs encapsulation [190]. The engraftment of BM-MSCs within a GG hydrogel modified with GRGDS fibronectin-derived peptide as previously described [130] increased cell proliferation and metabolic activity, when compared to unmodified hydrogels.…”

Section: Combining Biomaterials and Cell Transplantation For Sci Tmentioning

confidence: 99%

“…The engraftment of BM-MSCs within a GG hydrogel modified with GRGDS fibronectin-derived peptide as previously described [130] increased cell proliferation and metabolic activity, when compared to unmodified hydrogels. Moreover, BM-MSCs secretome was positively influenced, as proven by the enhancement of the survival and differentiation of primary cultures of hippocampal neurons in vitro [190]. …”

Section: Combining Biomaterials and Cell Transplantation For Sci Tmentioning

confidence: 99%

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Hydrogels and Cell Based Therapies in Spinal Cord Injury Regeneration

Assunção‐Silva

Gomes

Sousa

et al. 2015

Stem Cells International

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139

138

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Spinal cord injury (SCI) is a central nervous system- (CNS-) related disorder for which there is yet no successful treatment. Within the past several years, cell-based therapies have been explored for SCI repair, including the use of pluripotent human stem cells, and a number of adult-derived stem and mature cells such as mesenchymal stem cells, olfactory ensheathing cells, and Schwann cells. Although promising, cell transplantation is often overturned by the poor cell survival in the treatment of spinal cord injuries. Alternatively, the therapeutic role of different cells has been used in tissue engineering approaches by engrafting cells with biomaterials. The latter have the advantages of physically mimicking the CNS tissue, while promoting a more permissive environment for cell survival, growth, and differentiation. The roles of both cell- and biomaterial-based therapies as single therapeutic approaches for SCI repair will be discussed in this review. Moreover, as the multifactorial inhibitory environment of a SCI suggests that combinatorial approaches would be more effective, the importance of using biomaterials as cell carriers will be herein highlighted, as well as the recent advances and achievements of these promising tools for neural tissue regeneration.

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“…In most cases, GG products need to be sterilised prior to use in cell culture and TE. Various techniques are in use for this purpose including autoclaving [34][35][36] , ethylene oxide treatment 37 , microfiltration 38 , and antibiotic supplements 39 . Although no studies directly compare these methods with respect to their impacts on GG, equivalent investigations using other polysaccharides [40][41][42][43] and ECM materials 40,44,45 provide a good indication of the likely effects on GG.…”

Section: Purification and Sterilisationmentioning

confidence: 99%

Tissue engineering with gellan gum

et al. 2016

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Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built. Disciplines Engineering | Physical Sciences and Mathematics Publication DetailsStevens, L. R., Gilmore, K. J., Wallace, G. Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built.

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Modulation of bone marrow mesenchymal stem cell secretome by ECM-like hydrogels

Cited by 52 publications

References 28 publications

Peptide modification of purified gellan gum

Peptide modification of purified gellan gum

Hydrogels and Cell Based Therapies in Spinal Cord Injury Regeneration

Tissue engineering with gellan gum

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