Modulation of arsenic toxicity-induced oxidative damage by coronatine pretreatment in sweet basil (Ocimum basilicum) seedlings

Abstract

Coronatine (COR), a functional analogue of jasmonates, affects the accumulation of defense-related metabolites in plants. However, information on how its effects may be mediated by heavy metal stress is scanty. This study was carried out to determine the changes in some physiological parameters in sweet basil (Ocimum basilicum L.) seedlings pretreated with COR under arsenic (As) toxicity. COR was applied to seedlings at the three-leaf stage at 50 and 100 nanomolars (nmol·L −1 ). Arsenic at 150 and 300 mol·L −1 was imposed after completion of COR treatment. Toxic effects of As were reflected by the reduction in growth parameters and photosynthetic pigments and induction of lipid peroxidation coupled with enhancing hydrogen peroxide and electrolyte leakage in leaves. Under As toxicity, plants treated with COR significantly increased the fresh mass and relative water content in leaves. The increase was accompanied by decreased lipid peroxidation and hydrogen peroxide content and maintenance of chlorophyll pigments. COR supplement also increased the accumulation of phenolic compounds and proline content in the leaf of As-treated seedlings. Pretreatment with COR significantly enhanced activities of catalase (CAT), superoxide dismutase (SOD), and guaiacol peroxidase (POD) in basil leaves under As treatment. The results suggested that COR might alleviate As toxicity in sweet basil by activating antioxidant enzymes and inducing conservative components, thereby preventing membrane peroxidation.Résumé : La coronatine (COR), un analogue fonctionnel des jasmonates, affecte l'accumulation des métabolites reliés à la défense chez les plantes. Cependant, il existe peu d'information sur la façon elle peut intervenir sous un stress métallique. Les auteurs ont cherché à déterminer les changements de certains paramètres physiologiques chez des plantules de basilic (Ocimum basilicum L.) prétraitées avec de la COR, sous des conditions de toxicité en arsenic (As). Ils ont appliqué la COR sur les plantules au stade trois feuilles, à 50 et 100 nanomolaire (nmol·L −1 ). Suite au traitement à la COR, ils ont appliqué de l'As à 150 et 300 mol·L −1 . Les effets de l'As se sont traduits par la réduction des paramètres de croissance et des pigments photosynthétiques, ainsi qu'une induction de peroxydation des lipides couplée avec une augmentation du peroxyde d'hydrogène et d'une perte d'électrolytes. Sous l'effet toxique de l'As, les plantes traitées avec la COR augmentent significativement leur poids frais et la teneur relative en eau dans leurs feuilles. L'accroissement s'accompagne d'une diminution de la peroxydation des lipides et de la teneur en peroxyde, ainsi qu'un maintien des pigments chlorophylliens. Un supplément de COR augmente également l'accumulation de substances phénoliques et de la teneur en proline dans la feuille des plantules traitées à l'As. Un prétraitement avec la COR augmente significativement les activités de la catalase (CAT), de la superoxyde dismutase (SOD) et de la peroxydase du gauaicol (POD) chez les feuilles du...

Beneficial role of Coronatine on the morphological and physiological responses of Cress Plants (Lepidium sativum) exposed to Silver Nanoparticle

“…Pretreatment with coronatine improved the toxicity of silver nanoparticles by reducing the H 2 O 2 content. The present findings are in agreement with the results of Zare Dehabadi et al (2013), who indicated that exogenous application of coronatine reduced MDA overproduction in sweet basil seedlings under arsenic stress (Zare Dehabadi et al 2013 ).…”

“…In the present study, a decrease in proline content was observed in cress plants exposed to silver nanoparticles, while coronatine treatment mitigated this reduction in proline content induced by silver nanoparticles. Our findings are consistent with the increase in proline observed with coronatine treatment in sweet basil plants under arsenic stress (Zare Dehabadi et al 2013 ).…”

Effects of Arsenic on Nutrient Accumulation and Distribution in Selected Ornamental Plants

“…Copper is a component of enzymes associated with electron transport and protection against superoxide-free radicals [32]. Arsenic toxicity has been shown to cause an increase in reactive oxygen species leading to membrane leakage [33]. In the current study shoot Cu was reduced by ≥83% and root Cu by 94%.…”

“…Arsenic exposure causes widespread damage to a number of essential plant functions i.e. electron transport, neutralizing free radicals, photosynthesis, and membrane integrity [33] [34] [39]. Offsets in the timing of when factors controlling detoxification, damage control, and the initiation of secondary systems as primary systems are damaged may result in a complex growth response.…”