Modulation of Arachidonic Acid Metabolism in the Rat Kidney by Resveratrol: Implications for Regulation of Blood Pressure

Abstract

Background and Objective:The objective of this study was to investigate the effects of resveratrol (RES) on Arachidonic acid (AA) metabolism in the kidney and its effect on arterial blood pressure, using spontaneously hypertensive rats (SHR) as a model system. Methods: Rats were exposed to either drinking water alone (control) or RES (20 or 40 mg kgG 1 ) added to drinking water for 7 weeks. Mean Arterial Pressure (MAP) was measured at 7-day intervals throughout the study. At the end of treatment, rats were euthanized, followed by preparation of kidney microsomes to measure enzymes involved in regulation of vasoactive metabolites: CYP4A, the key enzyme in the formation of 20-hydroxyeicosatetraenoic acid and the soluble epoxide hydrolase, which is responsible for the degradation of the vasodilator metabolites such as epoxyeicosatetraenoic acids. Effect of RES on kidney expression of CYP4A was also investigated by immunoblotting. Results: Treatment with RES resisted the progressive rise in MAP in the developing SHR in a dose-dependent manner. Consistent with these data, RES treatment led to significant reductions in both, the expression and activity of renal CYP4A isozymes, as well as the activity of soluble epoxide hydrolase (sEH). Conclusion: The presented studies show that RES modulates the metabolism of AA by both P450 enzymes and sEH in SHR rats, which may represent a novel mechanism by which RES protects SHR rats against the progressive rise in blood pressure.

Protection of resveratrol on acute kidney injury in septic rats

“…Animals (Scientific Procedures) Act, 1986. 7 According to the previous references with the Res dose, 8,9 the rats were randomly divided into five groups, 10 of each, including (1) sham group with the rats being received sham operation, (2) sham + Res group with the rats being received sham operation and intraperitoneally injected with Res (3 mg/kg), (3) CLP group with the rats being established as septic models by CLP, (4) CLP + Res (3 mg/kg) group with the rats being established as septic models by CLP and intraperitoneally injected with Res (3 mg/kg), and (5) CLP + Res (10 mg/kg) group with the rats being established as septic models by CLP and intraperitoneally injected with Res (10 mg/kg). The rats in sham + Res, CLP + Res (3 mg/kg), and CLP + Res (10 mg/kg) groups were intraperitoneally injected with Res according to the setting concentrations after 2 h of surgery.…”

“…Res and polydatin (PD) were the natural components extracted from the plants, which were the nonflavonoid phenol. As the antioxidant, Res was involved in functions such as dilating blood vessels; preventing and treating arteriosclerosis, cardiovascular, and cerebrovascular disease; and so on, [9][10][11][12] as well as highly anti-tumor activity and cancer prevention. [13][14][15][16][17] However, there were few research on Res in sepsis, which were without conclusion and needed further investigations.…”