Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase

Li, Jiaxu; Kinoshita, Toshinori; Pandey, Sona; Ng, Carl; Gygi, Steven P.; Shimazaki, Ken Ichiro; Assmann, Sarah M.

doi:10.1038/nature00936

Cited by 166 publications

(125 citation statements)

References 30 publications

Supporting

Mentioning

119

Contrasting

Unclassified

Order By: Relevance

“…The linkage established here between ABA perception and SnRK2s in ferns provides direct evidence that this pathway, which is critical for the regulation of seed dormancy, stomatal aperture, and other dehydration responsive processes (e.g., dehydrin induction) (30) in seed plants, extends back at least 360 million years to the emergence of ferns. We propose that the origins of the ABA signaling pathway critical for regulating seed dormancy are present in the fern lineage that evolved 60 million years before the first fossil evidence of seed dormancy (20).…”

Section: Resultsmentioning

confidence: 99%

Abscisic acid controlled sex before transpiration in vascular plants

McAdam

Brodribb

Banks

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Sexual reproduction in animals and plants shares common elements, including sperm and egg production, but unlike animals, little is known about the regulatory pathways that determine the sex of plants. Here we use mutants and gene silencing in a fern species to identify a core regulatory mechanism in plant sexual differentiation. A key player in fern sex differentiation is the phytohormone abscisic acid (ABA), which regulates the sex ratio of male to hermaphrodite tissues during the reproductive cycle. Our analysis shows that in the fern Ceratopteris richardii, a gene homologous to core ABA transduction genes in flowering plants [SNF1-related kinase2s (SnRK2s)] is primarily responsible for the hormonal control of sex determination. Furthermore, we provide evidence that this ABA-SnRK2 signaling pathway has transitioned from determining the sex of ferns to controlling seed dormancy in the earliest seed plants before being co-opted to control transpiration and CO 2 exchange in derived seed plants. By tracing the evolutionary history of this ABA signaling pathway from plant reproduction through to its role in the global regulation of plant-atmosphere gas exchange during the last 450 million years, we highlight the extraordinary effect of the ABA-SnRK2 signaling pathway in plant evolution and vegetation function.OST1 | fern | stomata | evolution | sex determination

show abstract

Section: Resultsmentioning

confidence: 99%

Abscisic acid controlled sex before transpiration in vascular plants

McAdam

Brodribb

Banks

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Unbound proteins were removed by washing three times in the same buffer. Immobilized proteins were incubated with 2 g of poly(A) ϩ RNA isolated from epimastigote stage of the parasite according to the manufacturer's instructions (Promega) and then washed and extracted as described (23). After that, the recovered RNA was reverse-transcribed with an oligo-(dT) 18 anchor, and then a second strand was synthesized using specific primers for the messengers that figure in Table I.…”

Section: Methodsmentioning

confidence: 99%

RNA Recognition Motif-type RNA-binding Proteins in Trypanosoma cruzi Form a Family Involved in the Interaction with Specific Transcripts in Vivo

Gaudenzi¹,

D’Orso²,

Frasch

2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

Trypanosomes, protozoan parasites from the order Kinetoplastida, have to deal with environmental changes during the interaction with their hosts. Trypanosoma cruzi, the causative agent of Chagas' disease, uses post-transcriptional mechanisms to regulate gene expression. However, few RNA-binding proteins involved in mRNA turnover control have been identified to date. In this work, an RNA recognition motif (RRM)-type RNA-binding protein family named T. cruzi RNAbinding protein (TcRBP) and composed of at least six members was identified. The genomic organization of four members revealed a head to tail arrangement within a region of 15 kilobase pairs. TcRBP members have a common RRM and different auxiliary domains with a high content of glycine, glutamine, and histidine residues within their N-and C-terminal regions. TcRBPs differ in their expression patterns as well as in their homoribopolymer binding interaction in vitro, although they preferentially recognize poly(U) and poly(G) RNAs. An interesting observation was the relaxed RNA-binding interactions with several trypanosome transcripts in vitro. In contrast, co-immunoprecipitation experiments of TcRBP-containing ribonucleoprotein complexes formed in vivo revealed a highly restricted binding interaction with specific RNAs. Several TcRBPcontaining complexes are stage-specific and, in some cases, bear the poly(A)-binding protein TcPABP1. Altogether, these results suggest that TcRBPs might be modulated in vivo, to favor or preclude the interaction with specific transcripts in a developmentally regulated manner.Trypanosoma cruzi, the etiological agent of Chagas' disease, is a unicellular digenetic protozoan parasite with a complex life cycle that alternates between an insect and a vertebrate host, with different replicative and infective stages in both organisms. The parasite has a wide range of mammalian hosts, from humans to wild and domestic animal species, that act as reservoirs (1). T. cruzi infection is established in the mammal by the insect-derived stage metacyclic trypomastigote that differentiates to the intracellular replicative amastigote stage. This parasite form differentiates to the infective trypomastigote in the mammal, which is released from cells and circulates in blood, infecting other cells and being eventually ingested by the insect with its blood meal. Ingested trypomastigotes differentiate to epimastigotes (the replicative insect stage) that migrate along the digestive tract until differentiation into metacyclic trypomastigotes that are eliminated with the feces closing the cycle. Because the parasite suffers continuous environmental changes, it needs to regulate the expression of many proteins to allow its rapid adaptation. In contrast to other eukaryotic organisms, trypanosomatids do not regulate gene expression at the classical level of transcription initiation (2), although an RNA polymerase II transcriptional complex was recently identified (3). Transcription in these organisms is polycistronic, and the main point of regulation of gene e...

show abstract

“…Upon phosphorylation by ABA-activated protein kinase, AKIP binds to mRNAs encoding dehydrins (involved in stress response) and relocalizes to speckle-like domains (Li et al, 2002). Thus, AKIP may sequester mRNAs under stress.…”

Section: Function Of Sfs In Abiotic Stress Responsementioning

confidence: 99%

Alternative Splicing at the Intersection of Biological Timing, Development, and Stress Responses

2013

View full text Add to dashboard Cite

High-throughput sequencing for transcript profiling in plants has revealed that alternative splicing (AS) affects a much higher proportion of the transcriptome than was previously assumed. AS is involved in most plant processes and is particularly prevalent in plants exposed to environmental stress. The identification of mutations in predicted splicing factors and spliceosomal proteins that affect cell fate, the circadian clock, plant defense, and tolerance/sensitivity to abiotic stress all point to a fundamental role of splicing/AS in plant growth, development, and responses to external cues. Splicing factors affect the AS of multiple downstream target genes, thereby transferring signals to alter gene expression via splicing factor/AS networks. The last two to three years have seen an ever-increasing number of examples of functional AS. At a time when the identification of AS in individual genes and at a global level is exploding, this review aims to bring together such examples to illustrate the extent and importance of AS, which are not always obvious from individual publications. It also aims to ensure that plant scientists are aware that AS is likely to occur in the genes that they study and that dynamic changes in AS and its consequences need to be considered routinely.

show abstract

Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase

Cited by 166 publications

References 30 publications

Abscisic acid controlled sex before transpiration in vascular plants

Abscisic acid controlled sex before transpiration in vascular plants

RNA Recognition Motif-type RNA-binding Proteins in Trypanosoma cruzi Form a Family Involved in the Interaction with Specific Transcripts in Vivo

Alternative Splicing at the Intersection of Biological Timing, Development, and Stress Responses

Contact Info

Product

Resources

About