Modulation of adenylate cyclase activity by thyrotropin in cultured thyroid cells

Verrier, Bernard; Mauchamp, Jean; Lissitzky, Serge; Bugeia, Jean-Claude; Pelassy, C

doi:10.1016/0014-5793(80)81168-9

Cited by 14 publications

(10 citation statements)

References 12 publications

(8 reference statements)

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“…Cells were cultured in the absence of TSH either in monolayer on the permeable base of culture chambers or as inside-out follicles in suspension . As previously described, TSH increases intracellular cAMP in the two systems (Verrier, Mauchamp & Lissitzky, 1980;. In culture chambers, stimulators are in contact either with apical or with basolateral membranes while in inside-out follicles only the apical pole is accessible.…”

Section: Introductionmentioning

confidence: 92%

Thyrotrophin and cyclic AMP regulation of thyroglobulin gene expression in cultured porcine thyroid cells

Chabaud

Chambard²,

Gaudry³

et al. 1988

Journal of Endocrinology

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The effects of thyrotrophin, cholera toxin and 8-chloro-cyclic AMP on thyroglobulin gene expression in cultured porcine thyroid cells were compared. Cells organized either into monolayers in culture chambers with porous bases or into inside-out follicles in suspension were used. Thyroglobulin mRNA content was measured by dot-blot hybridization, and thyroglobulin synthesis rate was determined by immunoprecipitation of [35S]thyroglobulin after 2 h labelling with [35S]methionine. Cholera toxin and 8-chloro-cyclic AMP increased the thyroglobulin mRNA content and thyroglobulin synthesis rate in the same ratio (approximately 3) as did thyrotrophin, showing that cyclic AMP mediates the effect of thyrotrophin on thyroglobulin gene expression. The culture chamber system provides for contact of the effectors with either the apical or the basolateral membrane. The addition of 0.02-0.1 mU thyrotrophin/ml on the basolateral side of the cell layer gave a maximal response whereas this response was not reached on the apical side even with the addition of 5 mU/ml. In contrast, cholera toxin and 8-chloro-cyclic AMP stimulated thyroid cells equally on both sides.

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Section: Introductionmentioning

confidence: 92%

Thyrotrophin and cyclic AMP regulation of thyroglobulin gene expression in cultured porcine thyroid cells

Chabaud

Chambard²,

Gaudry³

et al. 1988

Journal of Endocrinology

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“…In membrane preparations derived from these cells, TSH, isoproterenol and PGE2 stimulate adenylate cyclase activity [5]. This stimulation is increased (2-3-fold) in the presence of GTP (0.1 mM).…”

Section: Introductionmentioning

confidence: 96%

Specific inhibition by Na⁺ of TSH‐stimulated thyroid cell adenylate cyclase

1982

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“…Depending on the culture conditions, thyroid epithelial cells can organize themselves either into vesicle or follicle-like structures that mimic the in viva state (Mauchamp et al, 1979a,b;Verrier et al, 1980; entiated pig thyroid cells cultured on poly (L-1ysine)-treated surfaces has been previously demonstrated (Wadeleux et al, 1985;Garbi et al, 1988). Chemical and immunological studies have revealed the presence of type IV collagen and heparan-sulfate containing proteoglycan in thyroid cells differentiated into follicularlike structures (Wadeleux et al, 1984).…”

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confidence: 99%

Differential expression of thrombospondin, collagen, and thyroglobulin by thyroid‐stimulating hormone and tumor‐promoting phorbol ester in cultured porcine thyroid cells

Bellon

Chaqour

Antonicelli

et al. 1994

Journal Cellular Physiology

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In the present study, we have investigated the potential regulation of thyroglobulin (Tg) and extracellular matrix components synthesis by thyroid-stimulating hormone (TSH) and tetradecanoyl phorbol-13-acetate (TPA) on thyroid cells. Porcine thyroid cells isolated by trypsin-EGTA digestion of thyroid glands were maintained in serum containing medium on poly (L-lysine)-coated dishes. Cells differentiated into follicular or vesicular-like structures were distinguished by their ability to organify Na[125I] and to respond to TSH stimulation. After an incubation of the cells with radiolabeled proline or methionine, two major proteins were identified, p450-480 and p290 (so named because of their molecular masses). Tg (p290) synthesis was demonstrated by the synthesis of [131I]-labeled polypeptides with electrophoretic properties identical to those of authentic Tg molecules. P450-480 resolved to M(r) 190,000 under reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) conditions. It was identified as thrombospondin by its reactivity with a monoclonal anti-human thrombospondin and by peptide sequencing of some of its tryptic fragments that displayed identity to thrombospondin I. Collagen synthesis was demonstrated by the formation of radioactive hydroxyproline and by the synthesis of pepsin-resistant polypeptides ranging from M(rs) 120,000 to 200,000. When the cells were cultured in the presence of 100 nM TPA, the culture medium contents of thrombospondin and collagen were increased by 2.7 and 1.6-fold, respectively, whereas Tg content was decreased by a factor 3.9. In contrast, the acute treatment of control cells with TPA induced a decrease in both Tg and collagen content by factors 3.0 and 1.5, respectively, and an increase in thrombospondin content by a factor 2.5. In the presence of 100 nM TPA, TSH (1 mU/ml) did not counteract the stimulating effect of TPA on extracellular matrix components synthesis. In contrast, when cells were cultured in the presence of TSH alone at concentrations higher than 0.1 mU/ml, collagen and thrombospondin in the medium were decreased by a factor 2.0 and 1.9, respectively, and TSH preferentially activated Tg synthesis. However, no acute response to TSH was observed in cells incubated for 2 days without effectors (control cells). On TSH differentiated cells, TPA decreased both collagen and Tg accumulation by factors 1.2 and 1.8, respectively, whereas it increased the one of thrombospondin by a factor 2. These results, together with the stimulating effect of TPA on TSH mediated cell proliferation, argue for a role of thrombospondin in cell adhesion and migration events within the thyroid epithelium.

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Modulation of adenylate cyclase activity by thyrotropin in cultured thyroid cells

Cited by 14 publications

References 12 publications

Thyrotrophin and cyclic AMP regulation of thyroglobulin gene expression in cultured porcine thyroid cells

Thyrotrophin and cyclic AMP regulation of thyroglobulin gene expression in cultured porcine thyroid cells

Specific inhibition by Na⁺ of TSH‐stimulated thyroid cell adenylate cyclase

Differential expression of thrombospondin, collagen, and thyroglobulin by thyroid‐stimulating hormone and tumor‐promoting phorbol ester in cultured porcine thyroid cells

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Modulation of adenylate cyclase activity by thyrotropin in cultured thyroid cells

Cited by 14 publications

References 12 publications

Thyrotrophin and cyclic AMP regulation of thyroglobulin gene expression in cultured porcine thyroid cells

Thyrotrophin and cyclic AMP regulation of thyroglobulin gene expression in cultured porcine thyroid cells

Specific inhibition by Na+ of TSH‐stimulated thyroid cell adenylate cyclase

Differential expression of thrombospondin, collagen, and thyroglobulin by thyroid‐stimulating hormone and tumor‐promoting phorbol ester in cultured porcine thyroid cells

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Specific inhibition by Na⁺ of TSH‐stimulated thyroid cell adenylate cyclase