Modulation of Adenosine Receptors by [60]Fullerene Hydrosoluble Derivative in SK-N-MC Cells

Abstract

The most known fullerenes are spherical carbon compounds composed of 60 carbon atoms. C60 fullerenes have shown biochemical and biomedical properties in the last years such as as blockade of apoptosis and neuroprotection. The nucleoside adenosine has a neuroprotective role mainly due to inhibition of glutamate release, which is a neurotransmitter related to excitotoxicity and cell death. In the present work, we have determined the presence of adenosine receptors in SK-N-MC cells, a neuroepithelioma human cell line, and analyzed the effect of fullerenes in these receptors by using radioligand binding, immunoblotting, and quantitative real time PCR assays. Results demonstrated that SK-N-MC cells endogenously express adenosine receptors. Fullerene exposure of these cells did not affect cell viability measured by MTT reduction assay. However, adenosine A1 and A2A receptors were both increased in SK-N-MC cells after treatment. These results suggest for the first time the modulation of adenosine receptors after C60 fullerenes exposure.

[60]Fullerene derivative modulates adenosine and metabotropic glutamate receptors gene expression: a possible protective effect against hypoxia

“…In line with this, the observed increase in A 1 ARs gene expression by t3ss derivative, as it has been previously reported in SK-N-MC cells [23], could be related to the described protective role exerted by these receptors during hypoxic conditions [30]. …”

Fullerene derivatives as nano-additives in polymer composites

“…Tetracationic fullerene C60 derivative VII. 271 Specific interaction between fullerene and proteins at the molecular level was mentioned above (see, e.g., Ref. 278).…”

“…15). 191,271 One of the most striking proofs of the ability of fullerenes to interact with proteins is the formation of fullerene-specific antibodies obtained for the water-soluble C 60 and C 70 fullerene derivatives. 272 An X-ray study of a Fab fragment of an antibody showed that the fullerene-binding site contains amino acids of both chains.…”

Biological activity of fullerenes - reality and prospects

“…Так, показано, что концентрация аденозин-А1-и А2А-рецепторов в клетках SK-N-MC увеличивалась после обработки производными фул-лерена, при этом существенно не влияя на жизне-способность клеток [47].…”