Modulation of 1,25-Dihydroxyvitamin D3 Receptor Expression in HaCaT Keratinocytes

Reichrath, Jörg; Hügel, Ulrike; Klaus, Günter; Fusenig, N. E.; Rauterberg, E. W.

doi:10.1007/978-3-642-77817-9_4

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…The cellular content of the VDR changes with keratinocyte differentiation. 37 We have previously demonstrated the presence of VDR in HaCaT cells 38 and now show (Fig. 2) that VDR levels increase as a function of time in culture up to day 8 and decrease thereafter in the more differentiated cells.…”

Section: Resultsmentioning

confidence: 56%

Upregulation of the calcium-dependent protease, calpain, during keratinocyte differentiation

Garach‐Jehoshua¹,

Ravid

et al. 1998

British Journal of Dermatology

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Calpain is a ubiquitous neutral calcium-activated thiol protease that is implicated in various cellular functions including exocytosis, cell fusion, apoptosis and proliferation. The calpain system is composed of the enzymes mu-calpain and m-calpain and their endogenous inhibitor, calpastatin. We employed the spontaneously immortalized human HaCaT keratinocytes, which retain their ability to differentiate in vitro and in vivo, to study the modulation of the calpain system during keratinocyte differentiation. The cellular levels of keratinocyte differentiation markers and of the components of the calpain system were monitored by immunoblotting. Three established differentiation stimuli: increase in cell density as a function of time in culture, elevation of extracellular calcium concentration and exposure to 1,25-dihydroxyvitamin D3 enhanced the expression of the three keratinocyte differentiation markers keratin 10, involucrin and transglutaminase. The differentiation of HaCaT cells was accompanied by elevation of the components of the calpain system, although the pattern of increase varied according to the specific differentiation stimulus. A higher increase in calpains as compared with the increase in calpastatin suggests an increase in net calpain activity during differentiation. Such an increase may play a part in the differentiation process itself and/or in the regulation of key events in differentiating keratinocyte metabolism.

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Section: Resultsmentioning

confidence: 56%

Upregulation of the calcium-dependent protease, calpain, during keratinocyte differentiation

Garach‐Jehoshua¹,

Ravid

et al. 1998

British Journal of Dermatology

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“…Therefore, our findings do not support the hypothesis that increased levels of VDR and subsequent interaction with 1,25(OH) 2 D 3 may be a reason for the high percentage of apoptotic cells in SCCs. We and others have shown that cytokines and other factors that stimulate transmembrane‐signaling pathways alter VDR levels in keratinocytes and other cell types in vitro 33,34 . Surrounding stroma of SCCs often contains an increased number of inflammatory cells.…”

Section: Discussionmentioning

confidence: 98%

“…We and others have shown that cytokines and other factors that stimulate transmembrane-signaling pathways alter VDR levels in keratinocytes and other cell types in vitro. 33,34 Surrounding stroma of SCCs often contains an increased number of inflammatory cells. Excreted cytokines and inflammatory peptides of these cells may be a reason for increased VDR expression in adjacent tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the vitamin D system in cutaneous squamous cell carcinomas

et al. 2004

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“…52 We and others have shown that cytokines and other factors that stimulate transmembrane-signaling pathways alter VDR levels in keratinocytes and other cell types in vitro. 53,54 43 and Reichrath. 44 Therefore, if the overexpression of 1a-OHase results in an overproduction of biologically active calcitriol, the accumulation of this metabolite should inhibit growth and invasion of BCCs.…”

Section: Discussionmentioning

confidence: 99%