Modulation der pri‐miRNA‐Reifung durch ein die apikale Schleife bindendes Aptamer

The Tuberculosis Drug Streptomycin as a Potential Cancer Therapeutic: Inhibition of miR‐21 Function by Directly Targeting Its Precursor

“…Lunse et al reported an aptamer that inhibits pri-miRNA processing by targeting the apical loop domain of pri-miRNA. [12] Others [13,14] have also provided evidence that the small molecule enoxacin modulates miRNA processing by enhancing TRBP (TAR RNA binding protein) activity in Dicer processing. Although, these studies provide a proof of concept, additional studies for understanding the modes of action, specificity, toxicity, and pharmacodynamic and pharmacokinetic properties of these molecules are called for.…”

A Rapid Assay for miRNA Maturation by Using Unmodified pre‐miRNA

“…The groups of Mayer and Famulok have developed an aptamer targeting a pri-miRNA apical loop, which leads to inhibition of the Droshamediated cleavage of the pri-miR17-18a-19a-20a-19b-1 cluster in HeLa nuclear extracts. [9] Recently, we proposed the inhibition of the Dicer-mediated miRNA maturation step by small molecules binding to a specific pre-miRNA and thereby blocking the Dicer-mediated cleavage of scissile phosphodiester bonds. Towards this goal, we developed a homogenous assay of miRNA maturation, employing a doubly labeled pre-miRNA with a 5'-fluoresceine and a 3'-DABCYL moiety.…”

The Tuberculosis Drug Streptomycin as a Potential Cancer Therapeutic: Inhibition of miR‐21 Function by Directly Targeting Its Precursor

“…Lunse et al. reported an aptamer that inhibits pri‐miRNA processing by targeting the apical loop domain of pri‐miRNA 12. Others13, 14 have also provided evidence that the small molecule enoxacin modulates miRNA processing by enhancing TRBP (TAR RNA binding protein) activity in Dicer processing.…”