Modulating the Human Basophil Phenotype During Its Development and Maturation: Basophils Derived from In Vitro Cultures of CD34+ Progenitor Cells

MacGlashan, Donald W.

doi:10.1007/978-1-0716-0696-4_6

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…Contrary to the case of mouse basophils, the committed progenitors for human basophils remain less identified. Providing that human basophil-like cells can be induced by culturing CD34 + progenitor cells isolated from the adult human bone marrow in the presence of IL-3, progenitors possessing basophil-differentiation potential may be found in the CD34 + cells in the bone marrow ( 71 ). Similarly, human mast cells can be generated ex vivo by culturing CD34 + c-Kit + cells isolated from the peripheral blood ( 72 , 73 ), indicating the presence of mast cell-committed progenitors in the peripheral blood CD34 + c-Kit + cells.…”

Section: Basophil Differentiation Trajectory Uncovered With Scrna-seq...mentioning

confidence: 99%

Novel insights into the ontogeny of basophils

Miyake,

Ito,

Karasuyama

2024

Front. Allergy

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Basophils are the least common granulocytes, accounting for <1% of peripheral blood leukocytes. In the last 20 years, analytical tools for mouse basophils have been developed, and we now recognize that basophils play critical roles in various immune reactions, including the development of allergic inflammation and protective immunity against parasites. Moreover, the combined use of flow cytometric analyses and knockout mice has uncovered several progenitor cells committed to basophils in mice. Recently, advancements in single-cell RNA sequencing (scRNA-seq) technologies have challenged the classical view of the differentiation of various hematopoietic cell lineages. This is also true for basophil differentiation, and studies using scRNA-seq analysis have provided novel insights into basophil differentiation, including the association of basophil differentiation with that of erythrocyte/megakaryocyte and the discovery of novel basophil progenitor cells in the mouse bone marrow. In this review, we summarize the recent findings of basophil ontogeny in both mice and humans, mainly focusing on studies using scRNA-seq analyses.

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Section: Basophil Differentiation Trajectory Uncovered With Scrna-seq...mentioning

confidence: 99%

Novel insights into the ontogeny of basophils

Miyake,

Ito,

Karasuyama

2024

Front. Allergy

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“…Comme les autres lignées myéloïdes, les basophiles se développent à partir de cellules souches hématopoïétiques multipotentes médullaires CD34+ [25]. C'est à ce niveau que les basophiles normaux se différencient totalement puis quittent la moelle osseuse pour passer dans la circulation sanguine sous forme mature.…”

Section: Basophiles Humains Normauxunclassified

Le polynucléaire basophile : du contrôle de l’immunité à celui des leucémies

Arock

2022

Annales Pharmaceutiques Françaises

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…While IL-3 is clearly most important for basophil development from precursors, other growth factors are reported to facilitate expansion/function. For example, the FMS-like tyrosine kinase 3 ligand (Flt3L) has been combined with IL-3 to expand the number of culture-derived basophils ( 33 ). Siracusa and co-workers reported that mouse basophils can be generated by thymic stromal lymphopoietin (TSLP) through the engagement of the heterodimeric TSLP receptor (TSLPR/IL-7Rα) ( 34 ).…”

Section: Introductionmentioning

confidence: 99%

Basophils from allergy to cancer

et al. 2022

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Human basophils, first identified over 140 years ago, account for just 0.5-1% of circulating leukocytes. While this scarcity long hampered basophil studies, innovations during the past 30 years, beginning with their isolation and more recently in the development of mouse models, have markedly advanced our understanding of these cells. Although dissimilarities between human and mouse basophils persist, the overall findings highlight the growing importance of these cells in health and disease. Indeed, studies continue to support basophils as key participants in IgE-mediated reactions, where they infiltrate inflammatory lesions, release pro-inflammatory mediators (histamine, leukotriene C4: LTC4) and regulatory cytokines (IL-4, IL-13) central to the pathogenesis of allergic diseases. Studies now report basophils infiltrating various human cancers where they play diverse roles, either promoting or hampering tumorigenesis. Likewise, this activity bears remarkable similarity to the mounting evidence that basophils facilitate wound healing. In fact, both activities appear linked to the capacity of basophils to secrete IL-4/IL-13, with these cytokines polarizing macrophages toward the M2 phenotype. Basophils also secrete several angiogenic factors (vascular endothelial growth factor: VEGF-A, amphiregulin) consistent with these activities. In this review, we feature these newfound properties with the goal of unraveling the increasing importance of basophils in these diverse pathobiological processes.

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Modulating the Human Basophil Phenotype During Its Development and Maturation: Basophils Derived from In Vitro Cultures of CD34+ Progenitor Cells

Cited by 3 publications

References 30 publications

Novel insights into the ontogeny of basophils

Novel insights into the ontogeny of basophils

Le polynucléaire basophile : du contrôle de l’immunité à celui des leucémies

Basophils from allergy to cancer

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