Abstract:Bioelectricity plays a vital role in living organisms. Although electrical stimulation is introduced in the field of bone regeneration, the concept of a dose-response relationship between surface potential and osteogenesis is not thoroughly studied. To optimize the osteogenic properties of different surface potentials, a flexible piezoelectric membrane, poly(vinylidene fluoridetrifluoroethylene) [P(VDF-TrFE)], is fabricated by annealing treatment to control its β phases. The surface potential and piezoelectric… Show more
“…This osteoinductive effect may be mainly attributed to the polarization charge derived from the composite membrane surface. The persistent polarization charge generated by the residual polarization of ferroelectric biomaterials could positively promote osteoblast activity, as reported by our previous study28 and other reports 26,27,32,40. These results implied that the polarized BTO/P(VDF-TrFE) nanocomposite membranes had higher osteoinductivity than the PTFE membrane, which might represent a better material for enhancing bone regeneration.Figure 2Osteogenic differentiation of BM-MSCs on polarized nanocomposite membranes and commercially-available PTFE membranes.…”
Section: Resultssupporting
confidence: 74%
“…The XRD patterns exhibited typical BTO crystallization characteristics and the ferroelectric β-phase of the P(VDF-TrFE) was detected (Figure 1C), which indicated that the crystalline characteristics of the piezoelectric ceramic BTO and the ferroelectric phase of the polymer P(VDF-TrFE) remained undisturbed during the fabrication process of the nanocomposite membrane. The β-phase in P(VDF-TrFE) has an important role in the piezoelectric and ferroelectric properties of the material31 and exert a positive effect on its biological functions 32Figure 1Characterizations of BTO/P(VDF-TrFE) nanocomposite membranes.…”
Purpose:
The combination of a bone graft with a barrier membrane is the classic method for guided bone regeneration (GBR) treatment. However, the insufficient osteoinductivity of currently-available barrier membranes and the consequent limited bone regeneration often inhibit the efficacy of bone repair. In this study, we utilized the piezoelectric properties of biomaterials to enhance the osteoinductivity of barrier membranes.
Methods:
A flexible nanocomposite membrane mimicking the piezoelectric properties of natural bone was utilized as the barrier membrane. Its therapeutic efficacy in repairing critical-sized rabbit mandible defects in combination with xenogenic grafts of deproteinized bovine bone (DBB) was explored. The nanocomposite membranes were fabricated with a homogeneous distribution of piezoelectric BaTiO
3
nanoparticles (BTO NPs) embedded within a poly(vinylidene fluoridetrifluoroethylene) (P(VDF-TrFE)) matrix.
Results:
The piezoelectric coefficient of the polarized nanocomposite membranes was close to that of human bone. The piezoelectric coefficient of the polarized nanocomposite membranes was highly stable, with more than 90% of the original piezoelectric coefficient (d
33
) remaining up to 28 days after immersion in culture medium. Compared with commercially-available polytetrafluoroethylene (PTFE) membranes, the polarized BTO/P(VDF-TrFE) nanocomposite membranes exhibited higher osteoinductivity (assessed by immunofluorescence staining for runt-related transcription factor 2 (RUNX-2) expression) and induced significantly earlier neovascularization and complete mature bone-structure formation within the rabbit mandible critical-sized defects after implantation with DBB Bio-Oss® granules.
Conclusion:
Our findings thus demonstrated that the piezoelectric BTO/P(VDF-TrFE) nanocomposite membranes might be suitable for enhancing the clinical efficacy of GBR.
“…This osteoinductive effect may be mainly attributed to the polarization charge derived from the composite membrane surface. The persistent polarization charge generated by the residual polarization of ferroelectric biomaterials could positively promote osteoblast activity, as reported by our previous study28 and other reports 26,27,32,40. These results implied that the polarized BTO/P(VDF-TrFE) nanocomposite membranes had higher osteoinductivity than the PTFE membrane, which might represent a better material for enhancing bone regeneration.Figure 2Osteogenic differentiation of BM-MSCs on polarized nanocomposite membranes and commercially-available PTFE membranes.…”
Section: Resultssupporting
confidence: 74%
“…The XRD patterns exhibited typical BTO crystallization characteristics and the ferroelectric β-phase of the P(VDF-TrFE) was detected (Figure 1C), which indicated that the crystalline characteristics of the piezoelectric ceramic BTO and the ferroelectric phase of the polymer P(VDF-TrFE) remained undisturbed during the fabrication process of the nanocomposite membrane. The β-phase in P(VDF-TrFE) has an important role in the piezoelectric and ferroelectric properties of the material31 and exert a positive effect on its biological functions 32Figure 1Characterizations of BTO/P(VDF-TrFE) nanocomposite membranes.…”
Purpose:
The combination of a bone graft with a barrier membrane is the classic method for guided bone regeneration (GBR) treatment. However, the insufficient osteoinductivity of currently-available barrier membranes and the consequent limited bone regeneration often inhibit the efficacy of bone repair. In this study, we utilized the piezoelectric properties of biomaterials to enhance the osteoinductivity of barrier membranes.
Methods:
A flexible nanocomposite membrane mimicking the piezoelectric properties of natural bone was utilized as the barrier membrane. Its therapeutic efficacy in repairing critical-sized rabbit mandible defects in combination with xenogenic grafts of deproteinized bovine bone (DBB) was explored. The nanocomposite membranes were fabricated with a homogeneous distribution of piezoelectric BaTiO
3
nanoparticles (BTO NPs) embedded within a poly(vinylidene fluoridetrifluoroethylene) (P(VDF-TrFE)) matrix.
Results:
The piezoelectric coefficient of the polarized nanocomposite membranes was close to that of human bone. The piezoelectric coefficient of the polarized nanocomposite membranes was highly stable, with more than 90% of the original piezoelectric coefficient (d
33
) remaining up to 28 days after immersion in culture medium. Compared with commercially-available polytetrafluoroethylene (PTFE) membranes, the polarized BTO/P(VDF-TrFE) nanocomposite membranes exhibited higher osteoinductivity (assessed by immunofluorescence staining for runt-related transcription factor 2 (RUNX-2) expression) and induced significantly earlier neovascularization and complete mature bone-structure formation within the rabbit mandible critical-sized defects after implantation with DBB Bio-Oss® granules.
Conclusion:
Our findings thus demonstrated that the piezoelectric BTO/P(VDF-TrFE) nanocomposite membranes might be suitable for enhancing the clinical efficacy of GBR.
“…Damaraju et al showed that PVDF scaffolds, which were prepared by electrospinning method and using the voltage of 25 KV, have the highest fraction of β‐phase and the biggest ALP activity and early mineralization for cultured MSCs, in comparison with controls and other voltages. Controlling the β phase content, Zhang et al modulated the surface potential of PVDF‐trifluoroethylene similar to bone tissue and enhanced the osteogenic differentiation of bone marrow mesenchymal stem cells. In an attempt to improve piezoelectric characteristics of the PVDF, Liu et al wrapped it by GO and showed that piezoelectric constant of PVDF/GO nanofibers is 426% higher than pure PVDF fibers.…”
Tissue engineering is fast becoming a key approach in bone medicine studies. Designing the ideally desirable combination of stem cells and scaffolds are at the hurt of efforts for producing implantable bone substitutes. Clinical application of stem cells could be associated with serious limitations, and engineering scaffolds that are able to imitate the important features of extracellular matrix is a major area of challenges within the field. In this study, electrospun scaffolds of polyvinylidene fluoride (PVDF), PVDF-graphene oxide (GO), PVDF-polyvinyl alcohol (PVA) and PVDF-PVA-GO were fabricated to study the osteogenic differentiation potential of human induced pluripotent stem cells (iPSCs) while cultured on fabricated scaffolds. Scanning electron microscopy study, viability assay, relative gene expression analysis, immunocytochemistry, alkaline phosphates activity, and calcium content assays confirmed that the osteogenesis rate of hiPSCs cultured on PVDF-PVA-Go is significantly higher than other scaffolds. Here, we showed that the biocompatible, nontoxic, flexible, piezoelectric, highly porous and interconnected three-dimensional structure of electrospun PVDF-PVA-Go scaffold in combination with hiPSCs (as the stem cells with significant advantageous in comparison to other types) makes them a highly promising scaffold-stem cell system for bone remodeling medicine. There was no evidence for the superiority of PVDF-GO or PVDF-PVA scaffold for osteogenesis, compared to each other; however both of them showed better potentials as to PVDF scaffold.
“…However, how to control the electrical stimulation to fit the physiological potential is still not clear. Z.C.G., et al (2018) [81] proposed a novel method to control surface potential of the bone membranes for enhancing and optimizing the regenerated bone tissue. Polyvinylidene fluoridetrifluoroethylene (PVDF-TRFE), a flexible and stable bone-healing material, was used as a template to investigate the relationship between osteogenic outcomes and surface potentials, as shown in Figure 8(D).…”
Biomineralization is a process in which organic matter and inorganic matter combine with each other under the regulation of living organisms. Because of the biomineralization-induced super survivability and retentivity, biomineralization has attracted special attention from biologists, archaeologists, chemists, and materials scientists for its tracer and transformation effect in rock evolution study and nanomaterials synthesis. However, controlling the biomineralization process in vitro as precisely as intricate biology systems still remains a challenge. In this review, the regulating roles of temperature, pH, and organics in biominerals forming process were reviewed. The artificially introducing and utilization of biomineralization, the bio-inspired synthesis of nanomaterials, in biomedical fields was further discussed, mainly in five potential fields: drug and cell-therapy engineering, cancer/tumor target engineering, bone tissue engineering, and other advanced biomedical engineering. This review might help other interdisciplinary researchers to bionic-manufacture biominerals in molecular-level for developing more applications of biomineralization.
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