Modulating Immunological Responses of Electrospun Fibers for Tissue Engineering

Goonoo, Nowsheen

doi:10.1002/adbi.201700093

Cited by 12 publications

(5 citation statements)

References 136 publications

(163 reference statements)

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“…It was noted that the gene expression of the M2 marker CD206 and the anti-inflammatory cytokines including IL-4 and IL-10 were enhanced by exosome-functionalized scaffolds, possibly because of the added influence of the scaffold’s microenvironment ( Fig. 1, F to H ) ( 22 , 23 ).…”

Section: Resultsmentioning

confidence: 99%

Mesenchymal stromal exosome–functionalized scaffolds induce innate and adaptive immunomodulatory responses toward tissue repair

Hao

Wang

et al. 2021

Sci. Adv.

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Designing scaffolds capable of inducing and guiding appropriate immune responses holds promise for tissue repair/regeneration. Biofunctional scaffolds were here prepared by immobilizing mesenchymal stromal exosomes onto fibrous polyester materials and allowed cell-mediated delivery of membrane-bound vesicles. Quantitative cell-level analyses revealed that immune cells dominated the uptake of exosomes from scaffolds in vivo, with materials and exosomes acting as the recruiter and trainer for immune cells, respectively, to synergistically promote beneficial macrophage and regulatory T cell responses in skin wounds in mice. Adaptive T helper cell responses were found active in remote immune organs, and exosome-laden scaffolds facilitated tissue repair in large skin injury models. This study demonstrated important mechanisms involved in local and systemic immune responses to biological implants, and understanding tissue-reparative immunomodulation may guide the design of new biofunctional scaffolds.

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Section: Resultsmentioning

confidence: 99%

Mesenchymal stromal exosome–functionalized scaffolds induce innate and adaptive immunomodulatory responses toward tissue repair

Hao

Wang

et al. 2021

Sci. Adv.

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“…A limitation of the degradation study was that only functional mechanical performance was evaluated and not mass loss, which would have added value in the current study. The difference in cell infiltration and subsequent amount of peritracheal reactive mass between the PCL and PCL/PLCL nanofibers may potentially be attributed to different inflammatory responses to the materials [42]. The faster-degrading PLCL material may potentially have less inflammatory tissue due to a faster degradation time compared to PCL.…”

Section: Discussionmentioning

confidence: 99%

Biodegradable electrospun patch containing cell adhesion or antimicrobial compounds for trachea repair in vivo

Townsend¹,

Hukill²,

Fung³

et al. 2020

Biomed. Mater.

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Difficulty breathing due to tracheal stenosis (i.e. narrowed airway) diminishes the quality of life and can potentially be life-threatening. Tracheal stenosis can be caused by congenital anomalies, external trauma, infection, intubation-related injury, and tumors. Common treatment methods for tracheal stenosis requiring surgical intervention include end-to-end anastomosis, slide tracheoplasty and/or laryngotracheal reconstruction. Although the current methods have demonstrated promise for treatment of tracheal stenosis, a clear need exists for the development of new biomaterials that can hold the trachea open after the stenosed region has been surgically opened, and that can support healing without the need to harvest autologous tissue from the patient. The current study therefore evaluated the use of electrospun nanofiber scaffolds encapsulating 3D-printed PCL rings to patch induced defects in rabbit tracheas. The nanofibers were a blend of polycaprolactone (PCL) and polylactide-co-caprolactone (PLCL), and encapsulated either the cell adhesion peptide, RGD, or antimicrobial compound, ceragenin-131 (CSA). Blank PCL/PLCL and PCL were employed as control groups. Electrospun patches were evaluated in a rabbit tracheal defect model for 12 weeks, which demonstrated re-epithelialization of the luminal side of the defect. No significant difference in lumen volume was observed for the PCL/PLCL patches compared to the uninjured positive control. Only the RGD group did not lead to a significant decrease in the minimum cross-sectional area compared to the uninjured positive control. CSA reduced bacteria growth in vitro, but did not add clear value in vivo. Adequate tissue in-growth into the patches and minimal tissue overgrowth was observed inside the patch material. Areas of future investigation include tuning the material degradation time to balance cell adhesion and structural integrity.

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“…The phenotype of macrophages is generally used to evaluate the timing and magnitude of inflammation and is the most important determinant of the biological performance of biomaterials [16,26,27]. Pro-inflammatory macrophages (M1) are master immune cells responsible for recruiting other immune cells to the interface of implants, clearing foreign pathogens by phagocytosis, and activating both the complement and adaptive immune systems [28].…”

Section: Introductionmentioning

confidence: 99%

Fiber configuration determines foreign body response of electrospun scaffolds: in vitro and in vivo assessments

Ma,

Wang,

Feng

et al. 2024

Biomed. Mater.

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Biomaterial scaffolds boost tissue repair and regeneration by providing physical support, delivering biological signals and/or cells, and recruiting endogenous cells to facilitate tissue-material integration and remodeling. Foreign body response (FBR), an innate immune response that occurs immediately after biomaterial implantation, is a critical factor determining the biological outcomes of biomaterial scaffolds. Electrospinning is of great simplicity and cost-effectiveness to produce nanofiber scaffolds with well-defined physicochemical properties and has been used in a variety of regenerative medicine applications in preclinical trials and clinical practice. A deep understanding of causal factors between material properties and FBR of host tissues is beneficial to the optimal design of electrospun scaffolds with favorable immunomodulatory properties. We herein prepared and characterized three electrospun scaffolds with distinct fiber configurations and investigate their effects on FBR in terms of immune cell-material interactions and host responses. Our results show that electrospun yarn scaffold results in greater cellular immune reaction and elevated FBR in in vivo assessments. Although the yarn scaffold showed aligned fiber bundles, it failed to induce cell elongation of macrophages due to its rough surface and porous grooves between yarns. In contrast, the aligned scaffold showed reduced FRB compared to the yarn scaffold, indicating a smooth surface is also a contributor to the immunomodulative effects of the aligned scaffold. Our study suggests that balanced porousness and smooth surface of aligned fibers or yarns should be the key design parameter of electrospun scaffolds to modulate host response in vivo.

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Modulating Immunological Responses of Electrospun Fibers for Tissue Engineering

Cited by 12 publications

References 136 publications

Mesenchymal stromal exosome–functionalized scaffolds induce innate and adaptive immunomodulatory responses toward tissue repair

Mesenchymal stromal exosome–functionalized scaffolds induce innate and adaptive immunomodulatory responses toward tissue repair

Biodegradable electrospun patch containing cell adhesion or antimicrobial compounds for trachea repair in vivo

Fiber configuration determines foreign body response of electrospun scaffolds: in vitro and in vivo assessments

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