Modulating <i>Vibrio cholerae</i> Quorum-Sensing-Controlled Communication Using Autoinducer-Loaded Nanoparticles

Lu, Hoang D.; Spiegel, Alina; Hurley, Amanda; Perez, Lark J.; Maisel, Katharina; Ensign, Laura M.; Hanes, Justin; Bassler, Bonnie L.; Semmelhack, M. F.; Prud'homme, Robert Krafft

doi:10.1021/acs.nanolett.5b00151

Cited by 46 publications

(49 citation statements)

References 58 publications

(98 reference statements)

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“…QS is regulated by the synthesis of small signaling molecules known as autoinducers (AIs), and by the synthesis of intracellular and membrane proteins that detect the AIs. QS monitors cell population density and plays a critical role in regulating diverse traits and mounting of orchestrated collective behaviors such as biofilm formation, bioluminescence and virulence production [21]. In many Gramnegative bacteria, QS is regulated by acyl-homoserine lactones (AHLs) that vary in the length of the alkyl side chain, or the presence of hydroxyl-or oxo-substituents.…”

Section: Introductionmentioning

confidence: 99%

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Qin

Engwer

Desai

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Highlights Chitosan-coated nanocapsules, but not nanoemulsions, influence the -potential of E.coli  A fixed number of nanocapsules binds and promotes aggregation of bacteria  In silico simulations agree closely with experimental results  Chitosan-coated nanocapsules attenuate the bacterial quorum sensing response § These authors contributed equally to this publication * Author for correspondence Colloids and Surfaces B: Biointerfaces 149 (2017) 358-368 AbstractWe examined the interaction between chitosan-based nanocapsules (NC), with average hydrodynamic diameter ~114-155 nm, polydispersity ~0.127, and -potential ~+50 mV, and an E. coli bacterial quorum sensing reporter strain. Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) allowed full characterization and assessment of the absolute concentration of NC per unit volume in suspension. By centrifugation, DLS, and NTA, we determined experimentally a "stoichiometric" ratio of ~80 NC/bacterium. By SEM it was possible to image the aggregation between NC and bacteria. Moreover, we developed a custom in silico platform to simulate the behavior of particles with diameters of 150 nm and -potential of +50 mV on the bacterial surface. We computed the detailed force interactions between NC-NC and NC-bacteria and found that a maximum number of 145 particles might interact at the bacterial surface. Additionally, we found that the "stoichiometric" ratio of NC and bacteria has a strong influence on the bacterial behavior and influences the quorum sensing response, particularly due to the aggregation driven by NC.

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Section: Introductionmentioning

confidence: 99%

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Qin

Engwer

Desai

et al. 2017

Colloids and Surfaces B: Biointerfaces

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“…This is especially important for optimizing cell targeting, as increasing ligand densities can improve binding in some instances, but excessively high-ligand densities can also result in decreased cell binding (Allen 2002;Cabral et al 2011;Cheng et al 2012;D'Addio et al 2013;Elias et al 2013;Kathleen et al 2007;Lee et al 2010). The FNP process enables the simultaneous encapsulation of several hydrophobic actives, including imaging modalities and hydrophobic drugs, to create theranostic NPs for novel antimicrobial treatments (Lu et al 2015(Lu et al , 2016(Lu et al , 2017Pansare et al 2012Pansare et al , 2014. We here present the development and characterization of FNPbased NPs that target Gram-negative and Gram-positive bacteria, and demonstrate proof-of-concept applications where bacteria are separated from media using commercially available and easily scalable magnetic separation setups.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle targeting of Gram-positive and Gram-negative bacteria for magnetic-based separations of bacterial pathogens

Yang

Wilson

et al. 2017

Appl Nanosci

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Antimicrobial resistance is a healthcare problem of increasing significance, and there is increasing interest in developing new tools to address bacterial infections. Bacteria-targeting nanoparticles hold promise to improve drug efficacy, compliance, and safety. In addition, nanoparticles can also be used for novel applications, such as bacterial imaging or bioseperations. We here present the use of a scalable block-copolymer-directed self-assembly process, Flash NanoPrecipitation, to form zinc(II)-bis(dipicolylamine) modified nanoparticles that bind to both Grampositive and Gram-negative bacteria with specificity. Particles have tunable surface ligand densities that change particle avidity and binding efficacy. A variety of materials can be encapsulated into the core of the particles, such as optical dyes or iron oxide colloids, to produce imageable and magnetically active bacterial targeting constructs. As a proof-of-concept, these particles are used to bind and separate bacteria from solution in a magnetic column. Magnetic manipulation and separation would translate to a platform for pathogen identification or removal. These magnetic and targeted nanoparticles enable new methods to address bacterial infections.

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“…Dense polyethylene glycol (PEG) steric layers minimize protein adsorption and slow reticuloendothelial clearance. 30,31 Also, the incorporation of targeting ligands on the end of a fraction of the PEG chains enables assembly of NPs with 5 precisely known targeting chain densities. Our earlier studies on NP drug delivery and optical imaging involved adding fluorescent dyes to the core of the NPs; however these particles were formed and characterized for fluorescent, and for not for PA applications.…”

Section: Introductionmentioning

confidence: 99%

Narrow Absorption NIR Wavelength Organic Nanoparticles Enable Multiplexed Photoacoustic Imaging

Wilson

Heinmiller

et al. 2016

ACS Appl. Mater. Interfaces

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Photoacoustic (PA) imaging is an emerging hybrid optical-ultrasound based imaging technique that can be used to visualize optical absorbers in deep tissue. Free organic dyes can be used as PA contrast agents to concurrently provide additional physiological and molecular information during imaging, but their use in vivo is generally limited by rapid renal clearance for soluble dyes and by the difficulty of delivery for hydrophobic dyes. We here report the use of the block copolymer directed self-assembly process, Flash NanoPrecipitation (FNP), to form series of highly hydrophobic optical dyes into stable, biocompatible, and water-dispersible nanoparticles (NPs) with sizes from 38 to 88 nm and with polyethylene glycol (PEG) surface coatings suitable for in vivo use. The incorporation of dyes with absorption profiles within the infrared range, that is optimal for PA imaging, produces the PA activity of the particles. The hydrophobicity of the dyes allows their sequestration in the NP cores, so that they do not interfere with targeting, and high loadings of >75 wt % dye are achieved. The optical extinction coefficients (ε (mL mg(-1) cm(-1))) were essentially invariant to the loading of the dye in NP core. Co-encapsulation of dye with vitamin E or polystyrene demonstrates the ability to simultaneously image and deliver a second agent. The PEG chains on the NP surface were functionalized with folate to demonstrate folate-dependent targeting. The spectral separation of different dyes among different sets of particles enables multiplexed imaging, such as the simultaneous imaging of two sets of particles within the same animal. We provide the first demonstration of this capability with PA imaging, by simultaneously imaging nontargeted and folate-targeted nanoparticles within the same animal. These results highlight Flash NanoPrecipitation as a platform to develop photoacoustic tools with new diagnostic capabilities.

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Modulating Vibrio cholerae Quorum-Sensing-Controlled Communication Using Autoinducer-Loaded Nanoparticles

Cited by 46 publications

References 58 publications

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules

Nanoparticle targeting of Gram-positive and Gram-negative bacteria for magnetic-based separations of bacterial pathogens

Narrow Absorption NIR Wavelength Organic Nanoparticles Enable Multiplexed Photoacoustic Imaging

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