Modulating effects of polysaccharides from the fruits of<i>Lycium barbarum</i>on the immune response and gut microbiota in cyclophosphamide-treated mice

Ding, Yu; Yan, Yamei; Chen, Dan; Ran, Linwu; Mi, Jia; Lu, Lu; Jing, Bo; Li, Xiaoying; Zeng, Xiaoxiong; Cao, Yang

doi:10.1039/c9fo00638a

Cited by 163 publications

(138 citation statements)

References 40 publications

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“…A recent study indicated that an imbalance of immune function was closely related to dysbiosis of the intestinal microbiota [3], as the gut barrier was disrupted and digestive system complications developed in the immunocompromised group [4]. The commensal microbiota plays an important role in the development and maintenance of the immune system and intestinal homeostasis via stimulating the immune response and maintaining the epithelial barrier functions [5]. Hence, gut microbiota dysbiosis is closely related to immunosuppression.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, gut microbiota dysbiosis is closely related to immunosuppression. For example, cyclophosphamide-induced immunosuppressed mice exhibited a decrease in the richness of bacterial species and an increase in the proportion of Bacteroidetes, while the proportion of Firmicutes and Proteobacteria increased [1,3,5]. This suggests that an imbalance of the immune system causes dysbiosis of the intestinal microbiota.…”

Section: Introductionmentioning

confidence: 99%

“…Polysaccharides are host-indigestible but can be metabolized by the gut microbiota. They are potential assets for the regulation of the gut microbiota as they promote the growth of beneficial bacteria (such as butyrate-producing bacteria) [5,9]. Citrus pectin is a complex polysaccharide that is widely used as a food additive.…”

Section: Introductionmentioning

confidence: 99%

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Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice

et al. 2020

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Subcritical water can effectively hydrolyze pectin into smaller molecules while still maintaining its functional regions. Pectic heteropolysaccharide can mediate immune regulation; however, the possible effects of subcritical water-hydrolyzed citrus pectin (SCP) on the immune response remain unclear. Therefore, the effects of SCP on immunomodulatory functions and intestinal microbial dysbiosis were investigated using a cyclophosphamide-induced immunosuppressed mouse model. In this research, immunosuppressed ICR mice were administrated with SCP at dosages of 300/600/1200 mg/kg.bw by oral gavage, and body weight, immune organ indexes, cytokines, and gut microbiota were determined. The results showed that subcritical water treatment decreased the molecular mass and increased the content of galacturonic acid in citrus pectin hydrolysates. Meanwhile, the treatment with SCP improved immunoregulatory functional properties and bioactivities over the original citrus pectin. For example, SCP protected immune organs (accelerated recovery of immune organ indexes) and significantly enhanced the expression of immune-related cytokines (IL-2, IL-6, IFN-γ, and TNF-α). The results of the 16S rDNA sequencing analysis on an IlluminaMiSeq platform showed that SCP normalized Cy-induced gut dysbiosis. SCP ameliorated Cy-dependent changes in the relative abundance of several taxa, shifting the balance back to normal status (e.g., SCP increased beneficial Muribaculaceae, Ruminococcaceae, Bacteroidaceae, and Prevotellaceae while decreasing pathogenic Brevundimonas and Streptococcus). The results of this study suggest an innovative application of citrus pectin as an immunomodulator.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice

et al. 2020

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“…It was found that TFPS could increase probiotic Lactobacillus and reduce risk Akkermansia , to maintain or improve the gut health. It was also found that polysaccharides form Lycium barbarum were capable of increasing the abundance of Lactobacillus (Ding et al., 2019). The common characteristic of these polysaccharides is that they are rich in uronic acid (Chen et al., 2019; Ding et al., 2019).…”

Section: Resultsmentioning

confidence: 99%

“…It was also found that polysaccharides form Lycium barbarum were capable of increasing the abundance of Lactobacillus (Ding et al., 2019). The common characteristic of these polysaccharides is that they are rich in uronic acid (Chen et al., 2019; Ding et al., 2019). It was noted that most of the bacteria showed no differences after long‐term intervention of TFPS, unlike observations in cyclophosphamide‐induced mice with disturbed gut microbiota (Chen et al., 2019).…”

Section: Resultsmentioning

confidence: 99%

Effect of long‐term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice

Chen

Ding

et al. 2020

Journal of Food Science

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Polysaccharides have various health‐promoting functions. However, dietary polysaccharides cannot be digested by the human alimentary tract, thus the gut is the most important location where polysaccharides play their role. The effect of polysaccharides from tea (Camellia sinensis L.) flower (TFPS) on intestinal health was investigated in the present study. TFPS with the molecular weight of 1,316.29 kDa was prepared, and twenty 6‐week‐old BALB/c male mice were randomly allotted to a chow diet (normal control group, NC group) or with 200 mg/kg (body weight)/day of TFPS for 13 weeks (n = 10 each). Histomorphology observation of jejunum and colons showed that TFPS maintained the adequate gut barrier. qPCR analysis revealed that the expression of colonic tight junction proteins of claudin1 (1.29 ± 0.15 compared with 1.00 ± 0.13, P < 0.05) and claudin5 (2.91 ± 0.44 compared with 1.00 ± 0.27, P < 0.01) at mRNA level with a significant difference between TFPS supplement or not, while the expression of TLR4 and TNF‐α mRNA was not changed statistically. 16S rDNA amplicons sequencing was applied to measure the compositions of gut microbiota from feces of mice. TFPS treatment exhibited similar relative abundances in Bacteroidetes and Firmicutes; however, it decreased the relative abundance of Akkermansia and increased that of Lactobacillus compared with the NC group. The contents of short‐chain fatty acids after TFPS supplementation, both in cecal contents and feces, were significantly higher than those of the NC group. Besides, TFPS significantly increased IgA production. These results suggest that TFPS is beneficial to intestinal health and can improve intestinal adaptive immune tolerance. Practical Application Dietary polysaccharides improve human intestinal health. Understanding the effect of TFPS, safe and healthy food components, on gut health increases the likelihood that TFPS will be developed as a functional food.

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Glucan from Dictyophora rubrovolvata boosts immunity by regulating AKT‐STAT1 pathway to induce dendritic cells maturation and T cells differentiation

Zhou

Feng

et al. 2023

Food Frontiers

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Glucan from fruit body of Dictyophora rubrovolvata (DRP) exerts immunomodulation activity, but the mechanism is still elusive. The immunoregulatory effects of DRP were evaluated using cyclophosphamide (CTX)‐induced immunosuppression in BALB/c mice. Furthermore, bone marrow dendritic cells were used to investigate the activated effect of DRP on immune cells in vitro. This study pointed out that DRP recuperated CTX‐induced immunosuppression of BALB/c mice and activated dendritic cells (DCs) to increase the secretion of cytokines in vitro. RNA‐sequencing showed that DRP upregulated the C‐type lectin receptor (CLR), AKT‐STAT1, and Th17 cell differentiation pathways in DCs. Furthermore, western blot and immunofluorescence confirmed that DRP increased the phosphorylation of AKT and STAT1 and the nuclear translocation of STAT1. Intriguingly, flow cytometry showed that the activation of DCs by DRP was interrupted by Mincle (a CLR) and STAT1 inhibitor. Furthermore, co‐cultured experiments demonstrated that DRP promoted naïve CD4+ T cell differentiation to Th17 cells via the stimulation of DCs. Hence, this study implied that DRP regulated the AKT‐STAT1 signaling pathway via Mincle in DCs to induce CD4+ T cell proliferation and differentiation, thereby ameliorating CTX‐induced immunosuppression.

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Modulating effects of polysaccharides from the fruits ofLycium barbarumon the immune response and gut microbiota in cyclophosphamide-treated mice

Abstract: The effects were investigated of Lycium barbarum polysaccharides on immunoregulation and gut microbiota dysbiosis in CTX-induced mice to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.

Cited by 163 publications

References 40 publications

Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice

Treatment with Subcritical Water-Hydrolyzed Citrus Pectin Ameliorated Cyclophosphamide-Induced Immunosuppression and Modulated Gut Microbiota Composition in ICR Mice

Effect of long‐term consumption of tea (Camellia sinensis L.) flower polysaccharides on maintaining intestinal health in BALB/c mice

Glucan from Dictyophora rubrovolvata boosts immunity by regulating AKT‐STAT1 pathway to induce dendritic cells maturation and T cells differentiation

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