Modulated 3D Cross-Correlation Dynamic Light Scattering of Magnetic Nanoparticle Inks

2019 21st International Conference on Transparent Optical Networks (ICTON)

2019

Self Cite

The deep biomedical knowledge reached in the past decades in many fields of medicine and biology has been accompanied by continuous technological progress in measurement instrumentation and analysis techniques. This development is important also to improve the characterization and the understanding of the interactions between nanoparticles and biological liquids, due to emerging applications such as drug delivery, bioimaging, biosensing, diagnostics and photothermal therapy. In this study, we characterize the interaction between mouse serum and gold nanoparticles (NPs) and nanorods (NRs) by Modulated 3D Cross-correlation Dynamic Light Scattering (DLS), in order to demonstrate that this technique can be applied to the investigation of complex biological liquids. The analysis of the size distribution of the hydrodynamic radius reveals three different contributions from particles motion, associated to rotation, translation and agglomerates. Moreover, we show that the interaction between Au NPs or NRs and mouse serum depends on the aspect ratio of the Au particles. These results are promising for deepening the knowledge on proteins-nanoparticles interaction, for laboratory-based experiments as well as for sensing and diagnostic applications and nanoparticles based medical therapy.

Section: Introductionmentioning

confidence: 99%

Characterization of Biological Liquids by Modulated 3D Cross-Correlation Dynamic Light Scattering

2019 21st International Conference on Transparent Optical Networks (ICTON)

2019

Self Cite

“…High-speed intensity modulators, with a frequency of 5 MHz, and the scattering angle of 90° are used. In this configuration multiple scattering events are significantly reduced [6], and even turbid samples can be measured [7]. The NP sizing is based on Brownian motion and Rayleigh scattering from particles which are significantly smaller than the laser wavelength.…”

Section: Optical Characterization By Dynamic Light Scattering and Absmentioning

confidence: 99%

Towards Standards for Light Scattering Studies of Proteins Stability and Nanoparticle-Protein Interactions

2020 22nd International Conference on Transparent Optical Networks (ICTON)

2020

Self Cite

Protein-nanoparticle suspensions are nowadays widely studied for the development of medical and environmental biosensors. The complexity of interactions between nanoparticles and biological fluids, together with the increasing use of dynamic light scattering (DLS) for their characterization, support the need to develop common standards for DLS measurements and analysis, in order to enable a reliable comparison of measurement results. In this study, we use 3D cross-correlation Dynamic Light Scattering (3Dcc-DLS) for the characterization of gold nanoparticles (Au NP), with spherical and rod shape, stabilized with sodium citrate, and their interaction with bovine serum albumin (BSA). We show that for Au nanorods it is possible to distinguish the rotational and translational motion. Moreover, we monitor the interaction of Au NPs and BSA over time, in order to analyse the first step of protein corona formation. Our results show that Au nanorods interact more than Au nanospheres with BSA. From the evolution of the rotational and translational peaks we conclude that the proteins tend to bind on the long cylindrical side of the nanorods.

“…As a result, samples can be measured even at high concentration and the need of dilution tests in order to obtain reliable results is significantly reduced [3]. Using this method, we have recently studied nanoparticle suspensions and inks [8]. in particular time-dependent behavior, such as particle-particle interactions, protein-corona formation, or formation of agglomerates can thus directly be measured and monitored at high concentration [9,10].…”

Section: Dynamic Light Scattering and Modulated 3d Cross-correlation Dynamic Light Scatteringmentioning

confidence: 99%

Modulated 3D Cross-Correlation Dynamic Light Scattering Applications for Optical Biosensing and Time-Dependent Monitoring of Nanoparticle-Biofluid Interactions

2020

Applied Sciences

Self Cite

This paper reviews dynamic light scattering (DLS) of gold nanoparticle-protein interactions for the model protein bovine serum albumin (BSA), as well as in complex biofluids, at the example of mouse serum. DLS data of nanorods of various aspect ratio, of proteins and of mouse serum are discussed in terms of the analysis of their hydrodynamic radii, leading to the distinction of rotational and translational motion as well as to the detection of agglomerates. We address in particular advances obtained by modulated 3D cross correlation dynamic light scattering and recent progress using the CORENN algorithm for analysis of DLS data.