Modular Peptide Growth Factors for Substrate‐Mediated Stem Cell Differentiation

Lee, Jae Sam; Lee, Jae Sung; Johnson, Amy J. Wagoner; Murphy, William L.

doi:10.1002/anie.200901618

Cited by 46 publications

(54 citation statements)

References 19 publications

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“…Many stem cell types secrete inductive growth factors during their differentiation process. For example, hMSCs secrete FGF during proliferation 35 , bone morpho-genetic protein-2 (BMP-2) during osteogenic differentiation 36,37 and transforming growth factor-β (TGF-β) during chondrogenic differentiation 38,39 . Therefore, one can envisage an autocatalytic process in which stem cells can amplify their own change in phenotype by secreting inductive growth factors (Fig.…”

Section: Materials Dynamicsmentioning

confidence: 99%

Materials as stem cell regulators

2014

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The stem cell/material interface is a complex, dynamic microenvironment in which the cell and the material cooperatively dictate one another's fate: the cell by remodelling its surroundings, and the material through its inherent properties (such as adhesivity, stiffness, nanostructure or degradability). Stem cells in contact with materials are able to sense their properties, integrate cues via signal propagation and ultimately translate parallel signalling information into cell fate decisions. However, discovering the mechanisms by which stem cells respond to inherent material characteristics is challenging because of the highly complex, multicomponent signalling milieu present in the stem cell environment. In this Review, we discuss recent evidence that shows that inherent material properties may be engineered to dictate stem cell fate decisions, and overview a subset of the operative signal transduction mechanisms that have begun to emerge. Further developments in stem cell engineering and mechanotransduction are poised to have substantial implications for stem cell biology and regenerative medicine.

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Section: Materials Dynamicsmentioning

confidence: 99%

Materials as stem cell regulators

2014

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“…42 Previous studies have demonstrated an ability to systematically vary the peptide-mineral binding affinity and release. 42,43 Here, we chose to use the highest affinity mineral-binding sequence to extend mBMP release over a much longer time frame than rhVEGF release, as a clear demonstration of our dual release concept. Analysis of the release profile of the growth factors from mineral-coated b-TCP biomaterials showed that both rhVEGF and mBMP can be released for over 60 days in different mediums (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…3,43,45 In a study by SuarezGonzalez et al, rhVEGF released from mineral-coated b-TCP (e) Quantification of the number of blood vessels within the pores of the scaffold. *p < 0.009 relative to the no growth factor condition.…”

Section: Discussionmentioning

confidence: 99%

Controlled Multiple Growth Factor Delivery from Bone Tissue Engineering Scaffolds via Designed Affinity

Suárez-González

Lee

Diggs

et al. 2014

Tissue Engineering Part A

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It is known that angiogenesis plays an important role in bone regeneration and that release of angiogenic and osteogenic growth factors can enhance bone formation. Multiple growth factors play key roles in processes that lead to tissue formation/regeneration during natural tissue development and repair. Therefore, treatments aiming to mimic tissue regeneration can benefit from multiple growth factor release, and there remains a need for simple clinically relevant approaches for dual growth factor release. We hypothesized that mineral coatings could be used as a platform for controlled incorporation and release of multiple growth factors. Specifically, mineral-coated scaffolds were ''dip coated'' in multiple growth factor solutions, and growth factor binding and release were dictated by the growth factor-mineral binding affinity. Beta tricalcium phosphate (b-TCP) scaffolds were fabricated using indirect solid-free form fabrication techniques and coated with a thin conformal mineral layer. Mineral-coated b-TCP scaffolds were sequentially dipped in recombinant human vascular endothelial growth factor (rhVEGF) and a modular bone morphogenetic peptide, a mineral-binding version of bone morphogenetic protein 2 (BMP2), solutions to allow for the incorporation of each growth factor. The dual release profile showed sustained release of both growth factors for over more than 60 days. Scaffolds releasing either rhVEGF alone or the combination of growth factors showed an increase in blood vessel ingrowth in a dosedependent manner in a sheep intramuscular implantation model. This approach demonstrates a ''modular design'' approach, in which a controllable biologics carrier is integrated into a structural scaffold as a thin surface coating.

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“…Those methods have been used to include instructive cues such as adhesive proteins (Ragetly, et al, 2010;Truong, et al, 2012;Assmann, et al, 2013;Choi, et al, 2013), GFs (Crouzier, et al, 2011;Macdonald, et al, 2011;Tang, et al, 2013;Shah, et al, 2014), modular peptides (Lee, et al, 2009) and bioactive calcium phosphate particles (Zhou, et al, 2014). Controlling the biochemical environment and the incorporation of release system is manageable using sequential approaches;…”

Section: Integrative Approachmentioning

confidence: 99%

Towards the design of 3D multiscale instructive tissue engineering constructs: Current approaches and trends

Oliveira

Reis

Mano

2015

Biotechnology Advances

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The design of 3D constructs with adequate properties to instruct and guide cells both in vitro and in vivo is one of the major focuses of tissue engineering. Successful tissue regeneration depends on the favorable crosstalk between the supporting structure, the cells and the host tissue so that a balanced matrix production and degradation are achieved. Herein, the major occurring events and players in normal and regenerative tissue are overviewed. These have been inspiring the selection or synthesis of instructive cues to include into the 3D constructs. We further highlight the importance of a multiscale perception of the range of features that can be included on the biomimetic structures. Lastly, we focus on the current and developing tissue-engineering approaches for the preparation of such 3D constructs: top-down, bottom-up and integrative. Bottom-up and integrative approaches present a higher potential for the design of tissue engineering devices with multiscale features and higher biochemical control than top-down strategies, and are the main focus of this review.

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Modular Peptide Growth Factors for Substrate‐Mediated Stem Cell Differentiation

Cited by 46 publications

References 19 publications

Materials as stem cell regulators

Materials as stem cell regulators

Controlled Multiple Growth Factor Delivery from Bone Tissue Engineering Scaffolds via Designed Affinity

Towards the design of 3D multiscale instructive tissue engineering constructs: Current approaches and trends

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