Modular evolution of disseminated Inc 7-M plasmids encoding gentamicin resistance

Labigne-Roussel, A; Witchitz, J; Courvalin, Patrice

doi:10.1016/0147-619x(82)90060-9

Cited by 31 publications

(14 citation statements)

References 36 publications

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“…The probe specific for tetC hybridized with EcoRI fragment 4 of all the plasmids studied. (11) and share homology with pCFF04 (Fig. 2).…”

Section: Methodsmentioning

confidence: 99%

“…Derivation of DNA probes used for hybridization are listed in Table 3. Plasmid DNA was labeled with 32p in vitro (14) and was hybridized to DNA immobilized on nytran filters (11 were provided by the following laboratories: Bristol Labocin Cl and C2, gentamicin, and netilmicin); The Upjohn Co., ratories MOLECULAR EPIDEMIOLOGY OF TEM-3 (CTX-1) ,-LACTAMASE (Fig. 1 provides part of this analysis).…”

Section: Methodsmentioning

confidence: 99%

“…mont-Ferrand (Table 2). Nalidixic acid-or rifampin-resistant mutants of Escherichia coli K-12 C600 (E. Wollman) were used in transfer experiments, and Escherichia coli BM21 harboring plasmid pIP135 (Tra+ Inc7 or M Gmr Smr Spr Sur Tcr; 79.3 kb) was used for incompatibility studies (11).…”

Section: Methodsmentioning

confidence: 99%

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Molecular epidemiology of TEM-3 (CTX-1) beta-lactamase

Petit

Gerbaud

Sirot

et al. 1990

Antimicrob Agents Chemother

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A total of 33 clinical isolates encoding TEM-3 (CTX-1) from four French hospitals were studied. The strains belonged to seven species, Klebsiella pneumoniae (n = 24), Escherichia coli (n = 3), Serratia marcescens (n = 2), Citrobacterfreundii (n = 1), Enterobacter aerogenes (n = 1), Enterobacter cloacae (n = 1), and Klebsiella oxytoca (n = 1). All the strains harbored an Inc7 or M self-transferable plasmid with a size of approximately 85 kilobases. The plasmids had closely related EcoRI, HincH, HindIll, and PvuH restriction endonucleasegenerated patterns and conferred resistance to all P-lactams, except cephamycins and imipenem; to tetracycline, because of the presence of the genes blatem3 and tetC, respectively, as determined by hybridization with specific probes; and to sulfonamide. Depending on the presence or absence and level of expression of the genes aacA4, aadA, and dfrl and of insertion element IS15, four types of plasmids could be distinguished.Plasmid pCFF04, the prototype plasmid encoding TEM-3, was widespread and appeared, by Southern hybridization, as the progenitor of the other types of replicons. The plasmid epidemic responsible for dissemination of TEM-3 in clinical isolates of members of the family Enterobacteriaceae may have originated in S. marcescens since pCFF04 was first detected in this species.In 1983, Knothe et al. (9) described the first transferable resistance to broad-spectrum cephalosporins in clinical isolates of Klebsiella pneumoniae and Serratia marcescens. This new resistance phenotype was due to production of a broad-spectrum P-lactamase, SHV-2, which evolved from the chromosomal SHV-l enzyme (8). Since 1984, strains of K. pneumoniae that produce a novel, transferable, broadspectrum P-lactamase have been isolated in Clermont-Ferrand hospitals (21). The enzyme was designated CTX-1 because of its high hydrolytic activity against cefotaxime. It was later shown to be derived from the TEM-2 enzyme (23) and was therefore redesignated TEM-3. In an earlier study (22), we screened 490 members of the family Enterobacteriaceae isolated between 1984 and 1987 that exhibited an antibiotic resistance phenotype similar to that of the first TEM-3-producing strains (21). We concluded that P-lactam resistance via synthesis of TEM-3 spread into seven different bacterial genera and was due to dissemination of a single 85-kilobase (kb) Inc7 or M plasmid, pCFF04. Here we report the molecular characterization of the plasmids isolated in 33 clinical isolates representative of the TEM-3-producing strains obtained from Clermont-Ferrand hospitals and three other geographically distant French hospitals. MATERIALS AND METHODSBacterial strains and plasmids. Twenty-eight strains of members of the family Enterobacteriaceae (22) were selected to represent all species of bacteria involved, different times of isolation over a 3-year period, different wards in which infection with TEM-3-producing bacteria occurred, and different biotypes (API 50 CH; API System S.A., Montalieu-Vercieu, France) of the most important ...

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“…The probe specific for tetC hybridized with EcoRI fragment 4 of all the plasmids studied. (11) and share homology with pCFF04 (Fig. 2).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular epidemiology of TEM-3 (CTX-1) beta-lactamase

Petit

Gerbaud

Sirot

et al. 1990

Antimicrob Agents Chemother

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“…Variations in restriction patterns among the Peruvian plasmids and R27 and TP123 were much greater, reflecting perhaps greater evolutionary pressures on the plasmids from Peru. Modular evolution involving sequential deletion and stepwise acquisition of transposons has been proposed as a mechanism to explain variation among both IncFII (20) and IncM plasmids (17). Similar types of events were noted in the Peruvian IncHIl plasmids, namely, acquisition of Ap and Su resistance accompanied by an increase in size of an XbaI fragment from 17.5 to 25 kb.…”

mentioning

confidence: 66%

Variability of IncHI1 plasmids from Salmonella typhi with special reference to Peruvian plasmids encoding resistance to trimethoprim and other antibiotics

Taylor¹,

Chumpitaz²,

Goldstein³

1985

Antimicrob Agents Chemother

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“…These previous examples fit into a picture of limited macroevolution: insertions, deletions, and, possibly, rearrangements, mediated by transposons and insertion sequence elements (3,6,21). Often the genetic changes directly relate to a phenotypic change, especially the gain of one (or more) additional resistance gene(s) by a transposition event.…”

Section: Discussionmentioning

confidence: 99%

Genetic and physical characterization of IncM plasmid pBWH1 and its variance among natural isolates

Hopkins¹,

Mayer²,

Gilleece³

et al. 1986

J Bacteriol

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We present a genetic and physical characterization of the IncM plasmid pBWH1. A physical map was constructed for the enzymes EcoRI, BamHI, Sall, BglII, HindIII, MstII, and XhoI. A series of deletions and a series of subclones of pBWH1 were constructed and used to determine the locations on this map of the transfer region; the replication region; and the genes determining resistance to beta-lactamg, chlotaamphenicol, the sulfonamides, and gentamicin. We compared 51 different isolates, including isolates which had lost individual antibiotic resistances or the transfer phenotype, and showed that variations occureed in all regions of the plasmid genome. Frequently, correlations could be made between phenotypic variation and variation of the EcoRI fragments which contained the gene determining that phenotype.The plasmid pBWH1 is a 92-kilobase IncM plasmid that has a broad host range for gram-negative members of the family Enterobacteriacae and carries four antibiotic resistance genes. These genes encode resistance to the aminoglycosides gentamicin, tobramicin, and kanamycin; to ampicillin and carbenicillin; to chloramphenicol; and to the sulfonamides (16). We have been monitoring the evolution of this plasmid and its derivatives at one location. Over a 10-year period, we obtained over 2,000 different isolates of strains carrying this plasmid as we followed the epidemic spread of pBWH1 at the Brigham and Women's Hospital in Boston.We examined phenotypic changes and restriction fragment variations. Analysis of these variations in pBWH1 may extend an important technique in the field of molecular evolution. Restriction fragment variation of, for example, metazoan mitochondria is currently being used to ascertain phylogenetic relationships (7, 13). The results of these experiments can be compared with the relationships inferred from comparative morphology and the fossil record; but rholecular data are available only for the present. The molecular evolution of bacterial plasmids is much faster, with significant changes appearing over relatively brief periods. Thus it is possible to monitor changing populations of plasmids through real time, as well as to extensively sample a population at any given time.pBWH1 has been found in strains of six different genera of the family Enterobacteriacae. Screening of many of these isolates has shown that the full phenotype of pBWH1, as well as a standard pattern of EcoRI restriction enzyme fragments, has persisted from 1976 to the present. A number of isolates, however, have shoWn significant phenotypic variations, losing resistance to an antibiotic or the ability to transfer the plasmid. There have also been many variations in the pattern of EcoRI fragments. We refer to these isolates as the pBWH1 series of plasmids. In this report we present a genetic and restriction enzyme map of pBWH1 and discuss variations in natural isolates in relationship to this map. We * Corresponding author.show that the variations of EcoRI restriction enzyme sites indicate that variations occur throughout th...

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Modular evolution of disseminated Inc 7-M plasmids encoding gentamicin resistance

Cited by 31 publications

References 36 publications

Molecular epidemiology of TEM-3 (CTX-1) beta-lactamase

Molecular epidemiology of TEM-3 (CTX-1) beta-lactamase

Variability of IncHI1 plasmids from Salmonella typhi with special reference to Peruvian plasmids encoding resistance to trimethoprim and other antibiotics

Genetic and physical characterization of IncM plasmid pBWH1 and its variance among natural isolates

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