Modular engineering of Shiraia bambusicola for hypocrellin production through an efficient CRISPR system

Deng, Huaxiang; Liang, Wei-Yue; Fan, Tai‐Ping; Zheng, Xiaohui; Cai, Yujie

doi:10.1016/j.ijbiomac.2020.09.208

Cited by 14 publications

(12 citation statements)

References 48 publications

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“…The widespread applications of PQs have prompted considerable research efforts to elucidate and exploit their biosynthetic pathways for industrial production ( de Jonge et al, 2018 ; Hu et al, 2019 ). Initially, bioinformatic methods, such as genome walking and genome evolutionary analysis were used to screen for potential PQ gene clusters ( Chooi et al, 2017 ; de Jonge et al, 2018 ; Deng et al, 2020b ). Then these candidate PQs biosynthetic genes were verified by gene disruption and chemical structure comparison between wild-type strains and mutants ( Deng et al, 2017a ; Hu et al, 2019 ).…”

Section: Elucidating the Perylenequinone Biosynthetic Pathwaymentioning

confidence: 99%

“…None of these mutants could produce hypocrellin. Relative expression experiments have not detected transcription of adjacent genes ( Deng et al, 2017a , 2020b ), or the presence of their corresponding intermediates ( Deng et al, 2018 ). These findings remain to be confirmed by heterologous expression of these enzymes and substrate selectivity/product characterization.…”

Section: Elucidating the Perylenequinone Biosynthetic Pathwaymentioning

confidence: 99%

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Advances and perspectives on perylenequinone biosynthesis

et al. 2022

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Under illumination, the fungal secondary metabolites, perylenequinones (PQs) react with molecular oxygen to generate reactive oxygen species (ROS), which, in excess can damage cellular macromolecules and trigger apoptosis. Based on this property, PQs have been widely used as photosensitizers and applied in pharmaceuticals, which has stimulated research into the discovery of new PQs and the elucidation of their biosynthetic pathways. The PQs-associated literature covering from April 1967 to September 2022 is reviewed in three sections: (1) the sources, structural diversity, and biological activities of microbial PQs; (2) elucidation of PQ biosynthetic pathways, associated genes, and mechanisms of regulation; and (3) advances in pathway engineering and future potential strategies to modify cellular metabolism and improve PQ production.

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Section: Elucidating the Perylenequinone Biosynthetic Pathwaymentioning

confidence: 99%

Section: Elucidating the Perylenequinone Biosynthetic Pathwaymentioning

confidence: 99%

Advances and perspectives on perylenequinone biosynthesis

et al. 2022

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“…Although the metabolic pathways' biological synthesis of the hypocrellins is still unclear, submerged fermentation with S. bambusicola has excellent application prospects in producing hypocrellins [13]. In recent years, various methods have been developed to increase the yield of the hypocrellins through fermentation, such as genetic modification and mutagenesis of the strains, optimization of media and cultivation conditions, and chemical and physical induction [12][13][14][15]. Through fermentation, the yield of the hypocrellins reached 90-8700 mg/L [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, various methods have been developed to increase the yield of the hypocrellins through fermentation, such as genetic modification and mutagenesis of the strains, optimization of media and cultivation conditions, and chemical and physical induction [12][13][14][15]. Through fermentation, the yield of the hypocrellins reached 90-8700 mg/L [14,15]. Fungi can elaborate the various enzymes for synthesizing secondary metabolites, most of which are critical natural sources of medicines with broad application prospects.…”

Section: Introductionmentioning

confidence: 99%

Promotion of the Hypocrellin Yield by a Co-Culture of Shiraia bambusicola (GDMCC 60438) with Arthrinium sp. AF-5 Fungus

Yan

Wen

et al. 2021

Fermentation

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Hypocrellin is a natural 3,10-xylene-4,9-anthracene derivative compound that originates from the stroma of Shiraia bambusicola (S. bambusicola) and Hypocrella bambusae with excellent photobiological activities. Submerged fermentation with the mycelia of S. bambusicola is generally regarded as an ideal technology for hypocrellin production. This study developed a co-cultivation strategy for an obvious promotion of the hypocrellin yield by incubating S. bambusicola (GDMCC 60438) with the endophyte fungus Arthrinium sp. AF-5 isolated from the bamboo tissue. The results indicated that the yield of hypocrellin A (HA) reached a 66.75 mg/g carbon source after an 84-h co-cultivation of the two strains, which was a four-time increase of that by the fermentation only with the S. bambusicola. The microscope observation found that the mycelia of the two strains were intertwined with each other to form the mycelium pellets during the co-cultivation. Moreover, the mycelium pellets of the co-culture showed a contracted and slightly damaged morphology. The addition of H2O2 in the fermentation media could further increase the HA production by 18.31%.

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“…[1] Dihydrocyclohepta [de]naphthalene, which consists of a seven-membered carbocycle fused with naphthalene, is a common structure among such compounds. [2] A number of natural products and bioactive compounds contain such skeletons (Figure 1), [3] e. g., hypocrellin and shiraiachrome, both of which are members of the perylenequinone class of natural products.…”

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confidence: 99%

Palladium‐Catalyzed Heck/Insertion/Decarboxylation Domino Sequence: Synthesis of Dihydrocyclohepta[de]naphthalenes

Chen

Zhuang

et al. 2021

Adv Synth Catal

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A Pd-catalyzed cascade cyclization for the construction of fused dihydrocyclohepta [de] naphthalene skeletons was developed. The skeleton was assembled from 3-iodo-4H-chromen-4-one, norbornene and 8-bromo-1-naphthoic acid. Unlike the classic Catellani reaction, which involves the removal of norbornene, in the present system, norbornene acts as a building block for the construction of rigid nonplanar molecular architectures rather than as an ortho-directing transient mediator. This synthesis involves the formation of three CÀ C bonds via a one-pot process, and it is compatible of a series of substituents.

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Modular engineering of Shiraia bambusicola for hypocrellin production through an efficient CRISPR system

Cited by 14 publications

References 48 publications

Advances and perspectives on perylenequinone biosynthesis

Advances and perspectives on perylenequinone biosynthesis

Promotion of the Hypocrellin Yield by a Co-Culture of Shiraia bambusicola (GDMCC 60438) with Arthrinium sp. AF-5 Fungus

Palladium‐Catalyzed Heck/Insertion/Decarboxylation Domino Sequence: Synthesis of Dihydrocyclohepta[de]naphthalenes

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