Modular and hierarchical self-assembly of siRNAs into supramolecular nanomaterials for soft and homogeneous siRNA loading and precise and visualized intracellular delivery

Abstract

siRNA therapeutics are challenged by the homogenous and efficient loading, maintenance of biological activities, and the precise, dynamic and monitorable site-releasing. Herein, supramolecular nanomaterials of WP5⊃G-siRNA were constructed by modular...

Current research trends of nanomedicines

“…However, many challenges remain due to degradation by nucleases 344 , difficulty in encapsulation 345 , poor membrane penetration 346 , and off-target effects 347 . Nanocarriers are considered a viable strategy to facilitate the delivery of nucleic acids, i.e ., ASO 348 , siRNA 349 , 350 , miRNA 351 , and mRNA 352 . Especially, the FDA recently approved two nucleic acid-based therapeutics to combat the COVID-19 pandemic 353 , 354 , 355 .…”

Pillararene‐Based Stimuli‐Responsive Supramolecular Delivery Systems for Cancer Therapy

“…Notably, the DOI: 10.1002/adma.202313317 pioneering works of pillararenes first reported by Ogoshi and co-workers [14] have attracted tremendous attention in the past fifteen years due to their convenient synthetic routes, high production yields, rigid electron-rich structures, unique host-guest interactions, and tunable cavity sizes, which have been further explored and widely applied in supramolecular chemistry, materials science, and biomedicine. [15][16][17][18][19][20][21] Owing to the tunable noncovalent interactions and diverse functionalization features of pillararene, pillararene-based SDSs, such as single molecule therapeutic agents, host-guest complexes, supramolecular self-assembly, metal/nonmetal nanoparticles, and porous materials, can achieve precise targeted delivery and controlled release via the integration of functional moieties and pillararene-based control components, which is conducive to improving therapeutic efficacy and reducing side effect during tumor therapy. Notably, the dynamic binding modes of pillararenes with functional moieties are one of the most significant merits in the fabrication of smart pillararene-based SDSs.…”

“…Notably, the pioneering works of pillararenes first reported by Ogoshi and co‐workers [ 14 ] have attracted tremendous attention in the past fifteen years due to their convenient synthetic routes, high production yields, rigid electron‐rich structures, unique host – guest interactions, and tunable cavity sizes, which have been further explored and widely applied in supramolecular chemistry, materials science, and biomedicine. [ 15–21 ]…”

Target-mediated self-assembly of DNA networks for sensitive detection and intracellular imaging of APE1 in living cells

“…[10][11][12][13] However, the lack of efficient and non-toxic siRNA delivery vectors and the precise release mechanism seriously hinder the clinical application of RNAi technology. [14][15][16][17][18] Therefore, with the help of APE1, designing efficient nanocarriers of siRNA with a precise release mechanism may be an effective therapy approach, and how to achieve sensitive detection of APE1 and accurately release siRNA remains to be explored.…”