Modifying Effects of Dietary Factors on (−)-Epigallocatechin-3-gallate-induced Pro-matrix Metalloproteinase-7 Production in HT-29 Human Colorectal Cancer Cells

Kim, Mi-Hye; Murakami, Akira; Ohigashi, Hajime

doi:10.1271/bbb.70213

Cited by 28 publications

(18 citation statements)

References 42 publications

(57 reference statements)

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“…Similar effects were observed by Toegel et al [2012], who examined the infl uence of a Caesalpinia sappan extract on human bone chondrosarcoma SW1353 cells. What is worth mentioning is that contrary to the results of Kim et al [2007], who reported that EGCG stimulates the expression of MMP-7 in colorectal cancer cell line HT-29, our polyphenol extract proved to be an effective repressor of MMP-7 gene. The activity of MMP-7 is thought to promote tumor cell survival by shedding Fas receptor from the cell surface which is responsible for apoptosis of the cell [Ii et al, 2006].…”

Section: Discussioncontrasting

confidence: 98%

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

Szewczyk¹,

Lewandowska²,

Owczarek³

et al. 2014

Pol. J. Food Nutr. Sci.

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Key words: evening primrose, matrix metalloproteinases, cancer Evening primrose (Oenothera paradoxa Hudziok) seeds are a rich source of not only a valuable oil containing an essential fatty acid --linolenic acid (GLA) -but also polyphenols which can be obtained from the biomass remaining after oil pressing. The aim of our studies was to evaluate the infl uence of a polyphenol extract from defatted seeds of evening primrose on human colorectal adenocarcinoma Caco-2 cell proliferation and matrix metalloproteinases (MMPs) synthesis and activity. To assess the effect of evening primrose extract on Caco-2 cell proliferation, crystal violet staining and sulforhodamine B (SRB) assays were used whereas mRNA expression and activity of MMPs were evaluated by RT-PCR and gelatin zymography.The results revealed that the examined polyphenol extract had little infl uence on Caco-2 proliferation, but effectively in a time-and dose-dependent manner inhibited MMP-1, MMP-7, MMP-9 and MMP-14 mRNA synthesis induced by TNF- and TPA. Additionally, zymographic analysis revealed that after 24 h, the polyphenol extract at a concentration of 50 mol/L GAE caused a 10-fold reduction in MMP-9 synthesis. Moreover, this extract might be a potent inhibitor of MMP activity. The results showed that polyphenol extract from evening primrose inhibited PBMC-derived MMP-2 and MMP-9 enzymatic activity in dose-dependent manner. The obtained results indicate that the polyphenol extract from evening primrose seeds could be an inhibitor of proteases involved in tumor progression and metastasis.

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Section: Discussioncontrasting

confidence: 98%

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

Szewczyk¹,

Lewandowska²,

Owczarek³

et al. 2014

Pol. J. Food Nutr. Sci.

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“…Along a similar line, we found that EGCG enhanced the expression of pro-matrix metalloproteinase-7 by inducing oxidative stress in HT-29 human colorectal cancer cells (Kim et al 2007). Furthermore, a 1% GTP diet enhanced pro-inflammatory cytokines, aggravated colitis, and tended to promote colon carcinogenesis in DSSexposed colons, while it decreased the activities of superoxide dismutase (SOD) and catalase in non-treated mice .…”

mentioning

confidence: 54%

High-dose green tea polyphenols induce nephrotoxicity in dextran sulfate sodium-induced colitis mice by down-regulation of antioxidant enzymes and heat-shock protein expressions

Inoue

Akiyama

Maeda‐Yamamoto

et al. 2011

Cell Stress and Chaperones

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Previously, we reported that oral feeding of 1% green tea polyphenols (GTPs) aggravated the dextran sulfate sodium (DSS)-induced colitis in mice. In the present study, we assessed the toxicity of 1% GTPs in several organs from normal and DSS-exposed mice. Sixty-two male ICR mice were initially divided into four groups. Nontreated group (group 1, n=15) was given standard diet and water, GTPs (group 2, n=15) received 1% GTPs in diet and water, DSS (group 3, n=15) received diet and 5% DSS in water, and GTPs + DSS group (group 4, n=17) received 1% GTPs in diet and 5% DSS in water. We found that group 4 significantly increased (P<0.05) kidney weight, the levels of serum creatinine and thiobarbituric acid-reactive substances in both kidney and liver, as compared with those in group 3. The mRNA expression levels of antioxidant enzymes and heat-shock proteins (HSPs) in group 4 were lower than those of group 3. For instance, heme oxygenase-1 (HO-1), HSP27, and 90 mRNA in the kidney of group 4 were dramatically down-regulated as compared with those of group 3. Furthermore, 1% GTPs diet decreased the expression of HO-1, NAD(P)H:quinone oxidoreductase 1 (NQO1) and HSP90 in kidney and liver of non-treated mice. Taken together, our results indicate that high-dose GTPs diet disrupts kidney functions through the reduction of antioxidant enzymes and heat-shock protein expressions in not only colitis but also non-treated ICR mice.

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“…A positive correlation between Sdc-1 and MMP-7 serum expression was observed. Since MMP-7 proteolysis is an important mechanism of Sdc-1 shedding, and MMP-7 can be secreted by colorectal cancer cells and has a pivotal role in almost every stage of colon cancer progression (Kim et al , 2007), we speculate that increased MMP-7 levels lead to enhanced proteolysis of Sdc-1 in colorectal cancer cells, and thus more soluble Sdc-1 is generated. In addition, following colon cancer resection, MMP-7 produced by colorectal cancer cell and MMP-7-induced Sdc-1 shedding was ceased.…”

Section: Discussionmentioning

confidence: 99%

Shed Syndecan-1 is involved in chemotherapy resistance via the EGFR pathway in colorectal cancer

et al. 2014

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Background:Syndecan-1 (Sdc-1) shedding induced by matrix metalloproteinase-7 (MMP-7) and additional proteases has an important role in cancer development. However, the impact of Sdc-1 shedding on chemotherapeutic resistance has not been reported.Methods:We examined Sdc-1 shedding in colorectal cancer by enzyme-linked immunosorbent assay (ELISA), Dot blot, reverse transcription-PCR (RT-PCR), immunohistochemistry and so on, its impact on chemotherapeutic sensitivity by collagen gel droplet embedded culture-drug sensitivity test (CD-DST) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), and potential mechanisms of action by Dot blot, western blot and immunofluorescence.Results:Sdc-1 shedding was increased in colorectal cancer patients, Sdc-1 serum levels in postoperative patients were lower than in preoperative patients, but still higher than those observed in healthy adults. Patients with high preoperative Sdc-1 serum levels were less responsive to 5-Fluorouracil, Oxaliplatin, Irintecan, Cisplatin or Paclitaxel chemotherapy. Moreover, the disease-free survival of patients with high preoperative Sdc-1 serum levels was significantly poorer. The possible mechanism of chemotherapy resistance in colorectal cancer can be attributed to Sdc-1 shedding, which enhances EGFR phosphorylation and downstream signalling.Conclusions:Shed Sdc-1 is involved in chemotherapy resistance via the EGFR pathway in colorectal cancer, and Sdc-1 serum levels could be a new prognostic marker in colorectal cancer.

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Modifying Effects of Dietary Factors on (−)-Epigallocatechin-3-gallate-induced Pro-matrix Metalloproteinase-7 Production in HT-29 Human Colorectal Cancer Cells

Cited by 28 publications

References 42 publications

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

High-dose green tea polyphenols induce nephrotoxicity in dextran sulfate sodium-induced colitis mice by down-regulation of antioxidant enzymes and heat-shock protein expressions

Shed Syndecan-1 is involved in chemotherapy resistance via the EGFR pathway in colorectal cancer

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