Modifying decellularized aortic valve scaffolds with stromal cell-derived factor-1α loaded proteolytically degradable hydrogel for recellularization and remodeling

Dai, Jinchi; Qiao, Weihua; Shi, Jing; Liu, Chungen; Hu, Xingjian; Dong, Nianguo

doi:10.1016/j.actbio.2019.02.002

Cited by 40 publications

(31 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of the reported problems identified with the use of decellularized tissue scaffolds is the accelerated degeneration with lack of cell repopulation and remodeling evidence [126]. Recently, Dai et al fabricated a scaffold of a porous matrix metalloproteinase degradable poly (ethylene glycol) hydrogel and decellularized porcine aortic valve in a rat subdermal model [127]. The hydrogel was loaded with stromal cell-derived factor-1α (SDF-1α) which, consequently, served to turn the decellularized scaffold biologically active.…”

Section: Biomaterialsmentioning

confidence: 99%

“…Another study also demonstrated that SDF-1α could control valve cell phenotype and is involved in scaffold recellularization and remodeling by stimulating the attraction of stem cells [129]. Dai et al results demonstrated that their hydrogel surface layers provided a niche for cell activities and helped protect the decellularized scaffold from rapid degeneration, inflammation, and calcification resulting in an improved recellularization and remodeling processes of the implanted decellularized heart valves [127].…”

Section: Biomaterialsmentioning

confidence: 99%

See 1 more Smart Citation

Biomaterials for In Situ Tissue Regeneration: A Review

2019

View full text Add to dashboard Cite

This review focuses on a somewhat unexplored strand of regenerative medicine, that is in situ tissue engineering. In this approach manufactured scaffolds are implanted in the injured region for regeneration within the patient. The scaffold is designed to attract cells to the required volume of regeneration to subsequently proliferate, differentiate, and as a consequence develop tissue within the scaffold which in time will degrade leaving just the regenerated tissue. This review highlights the wealth of information available from studies of ex-situ tissue engineering about the selection of materials for scaffolds. It is clear that there are great opportunities for the use of additive manufacturing to prepare complex personalized scaffolds and we speculate that by building on this knowledge and technology, the development of in situ tissue engineering could rapidly increase. Ex-situ tissue engineering is handicapped by the need to develop the tissue in a bioreactor where the conditions, however optimized, may not be optimum for accelerated growth and maintenance of the cell function. We identify that in both methodologies the prospect of tissue regeneration has created much promise but delivered little outside the scope of laboratory-based experiments. We propose that the design of the scaffolds and the materials selected remain at the heart of developments in this field and there is a clear need for predictive modelling which can be used in the design and optimization of materials and scaffolds.

show abstract

Section: Biomaterialsmentioning

confidence: 99%

Section: Biomaterialsmentioning

confidence: 99%

Biomaterials for In Situ Tissue Regeneration: A Review

2019

View full text Add to dashboard Cite

show abstract

“…How to achieve reendothelialization and reduce vascular thrombosis and the inflammatory stress of transplant recipients within a reasonable length of time are common burning issues faced by various decellularized scaffolds towards practical applications. To address these problems, researchers have tried to optimize the decellularized scaffolds though various modification methods, including immobilization of heparin (4,5), sugar (6), GRGDSPC peptides (7), anti-endothelial cell (EC) antibodies (8), and stromal cell-derived factor-1 alpha loaded proteolytically degradable hydrogel (9).…”

Section: Introductionmentioning

confidence: 99%

Heparin modification improves the re-endothelialization and angiogenesis of decellularized kidney scaffolds through antithrombosis and anti-inflammation in vivo

et al. 2021

View full text Add to dashboard Cite

Background: Constructing tissue-engineered kidneys using decellularized kidney scaffolds (DKS) has attracted widespread attention as it is expected to be the key to solving the shortage of donor kidneys.However, thrombosis and the host inflammatory response are unfavorable factors that hider the reendothelialization and vascularization of the decellularized scaffolds.Methods: Heparin was immobilized into the DKS using the method of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) activation. Fourier-transform infrared (FTIR) spectra were used to verify the heparinization of DKS. Human umbilical vein endothelial cells (HUVECs) were seeded and cultured in the DKS, then the sliced scaffolds were transplanted subcutaneously into nude mouse. Scanning electron microscopy and a series of histochemical stains including hematoxylin and eosin (H&E), elastic Verhöeff-Van Gieson (EVG), Sirius red, Masson's trichrome, and toluidine blue (TB) staining were used for morphological characterization. The qRT-PCR analysis, immunohistochemistry (IHC), and immunofluorescence (IF) staining were used to determine the expression of related molecular markers. Results:The rat DKS completely retained the extracellular matrix and heparinized modification. The H&E staining results showed there were more HUVECs covering the internal surfaces of tubular structures in the HEP-DKS group compared with the DKS group. The IF analysis results revealed that CD31, Ki67, and CD206 had higher positive rates in HUVECs in the HEP-DKS group compared to the DKS group. Both groups of scaffolds showed blood vessel formation via H&E staining, and there were more blood vessels in the HEP-DKS group compared with the native DKS group (P<0.05). The qRT-PCR results showed that the levels of IL-1β, IL-6, and TNF-α in the HEP-DKS group were significantly lower than those of the native DKS group, while the expression level of IL-10 was significantly higher than that in the native DKS group (P<0.05).Conclusions: Heparin modification improves the re-endothelialization and vascular regeneration of the DKS through anticoagulation in vitro and in vivo. The anti-inflammatory effect of heparin on the transplanted host was initially confirmed, and it is considered that this effect may play a non-negligible role in promoting DKS re-endothelialization and angiogenesis. Heparinized DKS is therefore a promising candidate for kidney tissue engineering.

show abstract

“…The combination of acellular porcine aortic value with porous matrix metalloproteinase (MMP) and degradable polyethylene glycol (PEG) hydrogel can mechanically promote bone marrow MSC (BM-MSC) attachment, growth, and differentiation. Consequently, recellularized MSCs promote constructive tissue remodeling by expressing the M2 macrophage phenotype, which could enhance the biocompatibility of transplanted values and inhibit their rapid destruction [ 113 ]. Moreover, Wang et al [ 69 ] confirmed that surface modification of the scaffold with gelatin or fibronectin enhances proper MSC attachment and proliferation in cardiac tissue engineering.…”

Section: Recellularization Of Scaffolds With Mscsmentioning

confidence: 99%

Recellularization of Native Tissue Derived Acellular Scaffolds with Mesenchymal Stem Cells

Ahmed

Saleh

2021

Cells

View full text Add to dashboard Cite

The functionalization of decellularized scaffolds is still challenging because of the recellularization-related limitations, including the finding of the most optimal kind of cell(s) and the best way to control their distribution within the scaffolds to generate native mimicking tissues. That is why researchers have been encouraged to study stem cells, in particular, mesenchymal stem cells (MSCs), as alternative cells to repopulate and functionalize the scaffolds properly. MSCs could be obtained from various sources and have therapeutic effects on a wide range of inflammatory/degenerative diseases. Therefore, in this mini-review, we will discuss the benefits using of MSCs for recellularization, the factors affecting their efficiency, and the drawbacks that may need to be overcome to generate bioengineered transplantable organs.

show abstract

Modifying decellularized aortic valve scaffolds with stromal cell-derived factor-1α loaded proteolytically degradable hydrogel for recellularization and remodeling

Cited by 40 publications

References 42 publications

Biomaterials for In Situ Tissue Regeneration: A Review

Biomaterials for In Situ Tissue Regeneration: A Review

Heparin modification improves the re-endothelialization and angiogenesis of decellularized kidney scaffolds through antithrombosis and anti-inflammation in vivo

Recellularization of Native Tissue Derived Acellular Scaffolds with Mesenchymal Stem Cells

Contact Info

Product

Resources

About