Modifying action of tritium on the charophytes bioelectrical response to anthropogenic pollution

Sevriukova, Olga; Kanapeckaite, Auste; Kisnierienë, Vilma; Ladygienė, Rima; Lapeikaite, Indre; Sakalauskas, Vidmantas

doi:10.5414/tex01326

Cited by 3 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study of comparatively low specific activity (7,15,30 and 65 kBq/L) of tritium effect on membrane bioelectrical parameters of algae N. obtusa cells shows that 15 kBq/L specific activity concentration increased the specific resistance of N. obtusa cells membrane, reduced current densities of Cl -and Ca 2+ in the depolarization phase of the action potential, and decelerated the recovery of the membrane action potential in the fast repolarization phase [24].…”

Section: Resultsmentioning

confidence: 99%

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

Marčiulionienė¹,

Jefanova²,

Sakalauskas³

et al. 2016

RadJ

View full text Add to dashboard Cite

Abstract. In the present study, we investigated the accumulation of 137 Cs and 90 Sr in compartments of the Nitellopsis obtusa cells. The effect of Sr 2+ , Cs + and Ca 2+ , K + , which are chemical analogues of 90 Sr and 137 Cs, to the bioelectric parameters of these algae were studied simultaneously. The aim of this work was studying the penetration of 137 Cs and 90 Sr through regulating membrane barriers in the cells of starry stonewort (Nitellopsis obtusa). 137 Cs and 90 Sr are accumulated mainly in the cell membrane (75% to 92%) of these algae. The cell membrane as a cation exchanger regulates ion flow through the first cells diffusion barrier -its thick outer cytomembrane (the complex consisting of the cell wall and plasmalemma). Significantly, smaller amounts of 137 Cs and 90 Sr enter into the cytoplasm than in the cytomembrane, 10-20% and 3-10%, respectively. Analysis of the accumulation levels of 137 Cs and 90 Sr in the compartments of the Nitellopsis obtusa cells show their accumulation in the cell membrane as well as their active transport through outer and inner cytoplasmic membranes. Membrane potentials determined mainly by the gradient of the K + ions are doing an important regulatory function in this process. From the obtained data it results that cells of Nitellopsis obtusa algae can be a convenient radioecological model for the study of the accumulation of radionuclides in plants at the cellular level.

show abstract

Section: Resultsmentioning

confidence: 99%

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

Marčiulionienė¹,

Jefanova²,

Sakalauskas³

et al. 2016

RadJ

View full text Add to dashboard Cite

show abstract

Radiocesium Phytotoxicity to Single Cell and Higher Plants

Marčiulionienė

Lukšienė

Montvydienė

et al. 2016

Impact of Cesium on Plants and the Environment

View full text Add to dashboard Cite

Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds

Kisnierienë

Lapeikaite

Pupkis

2018

Functional Plant Biol.

Self Cite

View full text Add to dashboard Cite

The Nitellopsis obtusa (N.A.Desvaux) J.Groves cell provides a model system for complex investigation of instantaneous effects of various biologically active compounds (BC) on the generation of plant bioelectrical signals in vivo. Experimental evidence using multiple electrical signals as biomarkers of the effects of BC (acetylcholine, asparagine, glutamate, nicotine, aluminium, nickel and cadmium ions) is provided. The effect of BC on membrane transport systems involved in the cell excitability were tested by current clamp, voltage clamp and patch clamp methods. Membrane potential (MP) alterations and action potential (AP) patterns in response to BC were shown to represent the cell state. High discretisation frequency allows precise, high time resolution analysis of real-time processes measuring changes in excitation threshold, AP amplitude and velocity of repolarisation values after application of BC indicating the effect on ion channels involved in AP generation. Application of voltage clamp revealed that changes in AP peak value were caused not only by increment in averaged maximum amplitude of the Clcurrent, but in prolonged Clchannels' opening time also. The cytoplasmic droplet can serve as a model system in which the effects of BC on single tonoplast ion channel can be studied by patch clamping. Investigation of electrical cell-to-cell communication revealed evidence on the electrical signal transduction through plasmodesmata.

show abstract

Modifying action of tritium on the charophytes bioelectrical response to anthropogenic pollution

Abstract: Objectives: Our living environment is composed of mixed pollution.

Cited by 3 publications

References 18 publications

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

Radiocesium Phytotoxicity to Single Cell and Higher Plants

Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds

Contact Info

Product

Resources

About

Modifying action of tritium on the charophytes bioelectrical response to anthropogenic pollution

Abstract: Objectives: Our living environment is composed of mixed pollution.

Cited by 3 publications

References 18 publications

THE ACCUMULATION PROCESS of137Cs AND90Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

THE ACCUMULATION PROCESS of137Cs AND90Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

Radiocesium Phytotoxicity to Single Cell and Higher Plants

Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds

Contact Info

Product

Resources

About

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE