Modify washing solutions in the process of deglycerolization in ACP 215 and storage at 4°C in 0.9% sodium chloride in 24h

Zhao, Yang; Luo, Guangping; Hong, Luo; Ye, Xin; Rong, Xia; Huang, Kejun

doi:10.1016/j.transci.2010.07.009

Cited by 2 publications

(3 citation statements)

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“…The loss of central pallor, increased RDW and MCV, and decreased MCHC with washing supports previous reports of human and canine RBC swelling after manual or automated RBC washing. 46 , 51 The cellular swelling during washing is widely considered to be secondary to build up of osmotically active substances (lactate, chloride, and 2,3‐DPG) intracellularly. 49 , 52 These osmotically active substances develop in RBCs during storage and, after washing, the extracellular osmolality decreases significantly depending on the washing media used, 53 , 54 resulting in an intracellular shift of fluid.…”

Section: Discussionmentioning

confidence: 99%

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Effect of washing units of canine red blood cells on storage lesions

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Ross

Williams

et al. 2021

Veterinary Internal Medicne

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Background In humans, washing stored blood products before transfusion reduces storage lesions and incidence of transfusion reactions, but the effectiveness of washing canine blood is unknown. Objectives The objective was to determine if manually washing units of stored blood would reduce storage lesions without adversely affecting erythrocytes. We hypothesized that washing stored units would reduce concentrations of storage lesions and cause minimal erythrocyte damage. Animals Eight healthy research dogs. Methods Repeated measure cohort study. Units of whole blood were stored for 28 days and washed 3 times with 0.9% NaCl. Blood samples were collected before and after storage, after each wash, and after being held at a simulated transfusion temperature. Variables measured included CBC variables, blood gas analysis, erythrocyte morphology, mean corpuscular fragility (MCF), and eicosanoid concentrations. A Friedman's test was used to evaluate changes in variables ( P < .05 was considered significant). Results After the first wash, compared to values after storage, there was a significant decrease in potassium (4.3 mmol/L [4.0‐4.7] to 1.2 mmol/L [1‐1.6]; P < .0001, median [range]), lactate (1.45 mmol/L [1.07‐1.79] to 0.69 mmol/L [0.39‐0.93]; P = .002), and partial pressure carbon dioxide (102 mm Hg [80.2‐119.2] to 33.7 mm Hg [24.5‐44.5]; P < .0001), and increase in MCV (69.3 fL [65.7‐72.3] to 74 fL [69.6‐79.5]; P = .0003), and MCF (0.444 fL [0.279‐0.527] to 0.491 fL [0.43‐0.616]; P = .0006). Conclusions and Clinical Importance A single wash of stored whole blood significantly reduces most extracellular storage lesions, and additional washing might cause hemolysis.

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Section: Discussionmentioning

confidence: 99%

“… 34 , 38 , 46 In humans, washing with solutions containing dextrose, mannitol, or albumin that exhibit osmotic or colloidal oncotic effects improves red cell morphology as evidenced by normalized MCV and increased MCHC, as well as decreased erythrocyte water content and improved cell health. 23 , 24 , 51 , 54 …”

Section: Discussionmentioning

confidence: 99%

Effect of washing units of canine red blood cells on storage lesions

Coll

Ross

Williams

et al. 2021

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…For instance, omission of prefreeze removal of excess glycerol [45] or spliting of blood units into pediatric units and consequent adjustment to a higher hematocrit [46] resulted in increased stability and low hemolysis and potassium, respectively. Furthermore, since deglycerolization leads to RBC loss, optimization of the existing protocols through reducing the washing duration by applying a mathematical formula [47], changing NaCl concentration for postwashing [48], and introducing a new dilution-filtration system [49] may represent promising alternatives. When small volumes of blood need to be frozen (mainly for future serological tests), droplet freezing, a method for RBC preservation in small, individual droplets is common.…”

Section: New Entries In the Cryofieldmentioning

confidence: 99%