Modified technique of Wolbachia removal from Malaysian Aedes albopictus

Joanne, Sylvia; Vythilingam, Indra; Yugavathy, Nava; Doss, Jonathan Inbaraj

doi:10.12980/apjtb.4.2014apjtb-2014-0020

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…Wolbachia free Ae. albopictus was maintained as described in Joanne et al (2014). Briefly, to remove Wolbachia, newly emerged adult mosquitoes were fed with 1.25 mg/mL tetracycline treated sucrose solution with B-Complex for the first two generations.…”

Section: Colonisation Of Ae Albopictus Free Of Wolbachiamentioning

confidence: 99%

Distribution and dynamics of Wolbachia infection in Malaysian Aedes albopictus

et al. 2015

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Section: Colonisation Of Ae Albopictus Free Of Wolbachiamentioning

confidence: 99%

Distribution and dynamics of Wolbachia infection in Malaysian Aedes albopictus

et al. 2015

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“…Wolbachia clearance from both mosquito species was conducted via tetracycline treatment of the adult mosquitoes as previously specified [ 44 ]. Briefly, adult mosquitoes were separated at the time of emergence into control ( Wolbachia (+)), and tetracycline-treated cohorts ( Wolbachia (-)), and provided with either sugar alone or sugar meals laden with 1.25 mg/ml of tetracycline respectively.…”

Section: Methodsmentioning

confidence: 99%

Impacts of fungal entomopathogens on survival and immune responses of Aedes albopictus and Culex pipiens mosquitoes in the context of native Wolbachia infections

et al. 2021

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Microbial control of mosquitoes via the use of symbiotic or pathogenic microbes, such as Wolbachia and entomopathogenic fungi, are promising alternatives to synthetic insecticides to tackle the rapid increase in insecticide resistance and vector-borne disease outbreaks. This study evaluated the susceptibility and host responses of two important mosquito vectors, Ae. albopictus and Cx. pipiens, that naturally carry Wolbachia, to infections by entomopathogenic fungi. Our study indicated that while Wolbachia presence did not provide a protective advantage against entomopathogenic fungal infection, it nevertheless influenced the bacterial / fungal load and the expression of select anti-microbial effectors and phenoloxidase cascade genes in mosquitoes. Furthermore, although host responses from Ae. albopictus and Cx. pipiens were mostly similar, we observed contrasting phenotypes with regards to susceptibility and immune responses to fungal entomopathogenic infection in these two mosquitoes. This study provides new insights into the intricate multipartite interaction between the mosquito host, its native symbiont and pathogenic microbes that might be employed to control mosquito populations.

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“…The Wolbachia -free JF.Neg was generated by treating adults with 1.25 mg/ml tetracycline dissolved in 5% sugar solution (32). After 4 generations, antibiotic-treated mosquitoes were 100% negative when analysed by qPCR, with no impact on general fitness, egg hatchability, larvae mortality and fecundity.…”

Section: Methodsmentioning

confidence: 99%

WolbachiastrainwAu efficiently blocks arbovirus transmission inAedes albopictus

Mancini

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et al. 2019

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10 11 The global incidence of arboviral diseases transmitted by Aedes mosquitoes, including dengue, 12 chikungunya, yellow fever, and Zika, has increased dramatically in recent decades. The release 13 of Aedes aegypti carrying the maternally inherited symbiont Wolbachia as an intervention to 14 control arboviruses is being trialled in several countries. However, these efforts are 15 compromised in many endemic regions due to the co-localization of the secondary vector 16 Aedes albopictus, the Asian tiger mosquito. Ae. albopictus has an expanding global distribution 17 following incursions into a number of new territories. To date, only the wMel and wPip strains 18 of Wolbachia have been reported to be transferred into and characterized in this vector. A 19 Wolbachia strain naturally infecting Drosophila simulans, wAu, was selected for transfer into 20 a Malaysian Ae. albopictus line to create a novel triple-strain infection. The newly generated 21 line showed self-compatibility, moderate fitness cost and complete resistance to Zika and 22 dengue infections. 23 24 Author summary 25 Aedes albopictus, the invasive Asian tiger mosquito, is responsible for numerous outbreaks of 26 important viruses such as dengue and Zika in various regions of the world. The need for 27 alterative control interventions propelled the development of a novel approach that exploits a 28 natural insect symbiont, Wolbachia; when transferred into non-native hosts, these maternally-29 inherited bacteria are able to interfere with the transmission of mosquito-borne viruses, and 30 also provide reproductive advantages to the host, offering a promising tool for self-sustaining 31 field applications. Currently, several field trials are ongoing for the primary vector of dengue 32 and several other arboviruses, Aedes aegypti, providing encouraging results. In this study, Ae.33 albopictus has been targeted for a similar approach: this mosquito species naturally carries two 34 strains of Wolbachia. The artificial introduction of a third, non-native strain made this line less 35 able to transmit dengue and Zika viruses and had an impact on its fitness. 37Introduction 38 The Asian tiger mosquito Aedes albopictus is an increasingly prominent vector of arboviruses.39 This anthropophilic and peridomestic mosquito species is an aggressive daytime biter with a 40 capacity to invade both temperate and tropical areas by adapting to different climates and 41 producing overwintering eggs. It originates in Asia, but is now widely distributed across 42 Europe, Africa, the Americas and the Pacific, and the identified transboundary mechanisms 43 involved in its expansion contribute to a high invasiveness and ever-increasing geographic 44 range (1, 2). 45 Ae. albopictus has been incriminated as a vector of more than 20 arboviruses (3); it acted as 46 the primary vector of some lineages of chikungunya virus (CHIKV) during outbreaks in La 47 Reunion (4, 5) and Italy (6). It is also a well-characterized secondary vector of dengue virus 48 (DENV) (7), with similar compe...

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Modified technique of Wolbachia removal from Malaysian Aedes albopictus

Abstract: This method is different from the previously published methods as it provides an improved Wolbachia removal technique from Ae. albopictus with high egg hatchability, low larvae mortality, increased fecundity and better Wolbachia removal rate.

Cited by 6 publications

References 13 publications

Distribution and dynamics of Wolbachia infection in Malaysian Aedes albopictus

Distribution and dynamics of Wolbachia infection in Malaysian Aedes albopictus

Impacts of fungal entomopathogens on survival and immune responses of Aedes albopictus and Culex pipiens mosquitoes in the context of native Wolbachia infections

WolbachiastrainwAu efficiently blocks arbovirus transmission inAedes albopictus

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