Modified Sugar Centrifugal Flotation Technique for Recovering Echinococcus multilocularis Eggs From Soil

Matsuo, Kayoko; Kamiya, Hajime

doi:10.1645/ge-3388rn

Cited by 13 publications

(10 citation statements)

References 18 publications

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“…Veterinary hospitals usually use this method based on cost, ease of use, availability of hardware, or simply tradition. However, this method came with high contamination and less sensitivity [19].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and risk factors associated with Eimeria species infection in cattle of different geographical regions of Indonesia

et al. 2021

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Background and Aim: Eimeria spp. are gastrointestinal protozoans that affect animal productivity, thereby causing symptoms that range from bloody diarrhea to death. These symptoms cause economic losses to farmers. The distribution of Eimeria spp. in cattle has, therefore, been reported to have spread widely, especially in the tropics and subtropics. Indonesia is a tropical country at high risk of Eimeria infections. This study aimed to identify the prevalence and risk factors related to the levels of eimeriosis in beef cattle originating from different geographic areas in Indonesia. Materials and Methods: Here, 817 fecal samples were collected from beef cattle in Indonesia, including 282 calves, 535 adults, 530 males, and 287 females. In addition, 156 semi-intensively and 661 intensively managed cattle were randomly collected. Then, fecal samples were analyzed by parasitology examinations. Results: Screening examination using the sugar flotation modification method showed that Eimeria spp. were prevalent in Indonesia, as 65.4% of the bacterial strain was detected. The prevalence of identified Eimeria spp. in Indonesia was highest in North Maluku (Maluku Island) (94.1%), whereas the lowest levels were observed in West Java (24.0%) (Java Island). The prevalence was also found to be higher in males (79.3%) than females (51.9%). Similarly, levels in semi-intensively managed cattle (66.7%) were higher than those subjected to intensive management (65.9%). However, its prevalence in calf and adult cattle was similar. Conclusion: Bovine eimeriosis spp. were detected at high prevalence in Indonesia, and high-level risks were observed in infected males, including those under the semi-intensive management. In addition, although the results from oocyst examinations were based on qualitative analysis, the endemicity levels of Eimeria spp. among farms in Indonesia should be considered because Eimeria spp. were distributed in most parts of Indonesia. Based on the results of this study, we provide the first information about the prevalence of bovine eimeriosis from different geographical locations in Indonesia, which have differing climates associated with the level of the existing risk factors. Hence, farmers are advised to pay more attention to strict biosecurity techniques on their farms, thereby favoring the early control of bovine eimeriosis.

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Section: Discussionmentioning

confidence: 99%

Prevalence and risk factors associated with Eimeria species infection in cattle of different geographical regions of Indonesia

et al. 2021

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“…Surveillance of infection in intermediate hosts based solely on abattoir surveillance will tend to under-represent or exclude animals from these communities, and efforts may therefore need to be made to address this. Although methods based upon egg extraction from soil samples have been applied for the direct estimation of levels of environmental contamination (Craig et al, 1988;Matsuo and Kamiya, 2005), these can be quite labour intensive -especially when considering large areas of possible contamination. Another approach to investigation of environmental contamination with Echinococcus spp.…”

Section: Surveillance Of Intermediate Hostsmentioning

confidence: 99%

Echinococcus granulosus: Epidemiology and state-of-the-art of diagnostics in animals

Craig

Mastin

Kesteren

et al. 2015

Veterinary Parasitology

119

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Diagnosis and detection of Echinococcus granulosus (sensu lato) infection in animals is a prerequisite for epidemiological studies and surveillance of echinococcosis in endemic, re-emergent or emergent transmission zones. Advances in diagnostic approaches for definitive hosts and livestock, however, have not progressed equally over the last 20 years. Development of laboratory based diagnostics for canids using coproantigen ELISA and also coproPCR, have had a huge impact on epidemiological studies and more recently on surveillance during hydatid control programmes. In contrast, diagnosis of cystic echinococcosis (CE) in livestock still relies largely on conventional post-mortem inspection, despite a relatively low diagnostic sensitivity especially in early infections, as current serodiagnostics do not provide a sufficiently specific and sensitive practical pre-mortem alternative. As a result, testing of dog faecal samples by coproantigen ELISA, often combined with mass ultrasound screening programmes for human CE, has been the preferred approach for monitoring and surveillance in resource-poor endemic areas and during control schemes. In this article we review the current options and approaches for diagnosis of E. granulosus infection in definitive and animal intermediate hosts (including applications in non-domesticated species) and make conclusions and recommendations for further improvements in diagnosis for use in epidemiological studies and surveillance schemes.

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“…-Modified Baermann technique: (Graeff-Teixeira et al 1997) -Charcoal culture: (Beaver et al 1984) Modified Wisconsin Sugar Flotation Method (Matsuo and Kamiya 2005) Physic-chemical study of the soil:…”

Section: Detection Of Different Parasitic Stagesmentioning

confidence: 99%

Geohelminths distribution as affected by soil properties, physicochemical factors and climate in Sharkyia governorate Egypt

et al. 2014

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Soil-transmitted helminths are mainly a group of parasitic nematodes causing human infection through contact with parasite eggs or larvae; they survive in the warm and moist soil of the tropical and subtropical countries. This study was carried out in Sharkyia governorate from October, 2011 to October, 2013, to correlate between the prevalence and distribution of these parasites in the soil and the physicochemical factors affecting the examined samples of the soil. One hundred and twenty samples of different types of soil (clay, silt, sand) from different localities were collected and examined. Diagnosis of geohelminths was confirmed by the recovery of their eggs and larvae with other protozoa by different parasitological methods. The modified baermann method was found to be more efficient in detection of geohelminths larvae than charcoal culture method. Among the examined sites geohelminths were much more numerous in the soil of rural areas especially in the spring and summer seasons, while the contamination of canal banks by geohelminths was the worst (80 %). An insignificant correlation was reported between the soil texture and the number of positive samples in the examined areas while the relationship was directly proportional among (moisture, PH, organic). It appeared that the most common geohelminthic stage was Toxocara spp. eggs besides other types of protozoa especially Balantidium coli cysts. This suggests that factors other than soil texture are important in the prevalence of geohelminths in the soil e.g. temperature, moisture, PH and organic matter. So, to change some of these factors in a trial to control geoparasites transmission but with keeping the environment should be tried. These results also open the way to further studies to highlight the mutual affection between inhabitants of these sites and the prevalence of these geoparasites.

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Modified Sugar Centrifugal Flotation Technique for Recovering Echinococcus multilocularis Eggs From Soil

Cited by 13 publications

References 18 publications

Prevalence and risk factors associated with Eimeria species infection in cattle of different geographical regions of Indonesia

Prevalence and risk factors associated with Eimeria species infection in cattle of different geographical regions of Indonesia

Echinococcus granulosus: Epidemiology and state-of-the-art of diagnostics in animals

Geohelminths distribution as affected by soil properties, physicochemical factors and climate in Sharkyia governorate Egypt

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