Modified Silica-Based Packing Materials for Size Exclusion Chromatography

Separation of Monoclonal Antibodies by Analytical Size Exclusion Chromatography

“…The secondary interactions are based on charge-related interactions or hydrophobicity-related interactions. The secondary interaction coming from the free silanol groups present on the silica surface of the stationary phase is often protected by a diol-bonded coating on the stationary phase which shields the silica surface from such action [14].…”

Preparation and characterization of nanocomposites based on polylactide manufactured by reactive extrusion-calendering : thermal, rheological, mechanical and fracture properties

“…The main degradation mechanisms include hydrolysis of the ester linkages by trace amounts of water, which occurs more or less randomly along the backbone of the polymer (Elsawy et al, 2017;Tsuji, 2010), zipper-like depolymerization catalysed by the remaining polymerization catalyst (Jamshidi et al, 1988;Sin et al, 2013b), oxidative, random main-chain scission (Gupta and Deshmukh, 1982;Johansson, 2016), intra and intermolecular transesterification reactions (Kopinke et al, 1996;Zhang et al, 1992). Last but not least, pyrolysis might also be considered as a depolymerization mechanism at elevated temperatures (>300 ºC) (Nishida et al, 2003).…”

“…Hexafluoroisopropanol (1,1,1,3,3,3-hexafluoro-2-propanol, HFIP) containing 0.02 mol.L -1 of sodium trifluoroisopropanol was used as the mobile phase to achieve isocratic elution and prevent the "polyelectrolyte effect". The calibration were performed by injecting a mixture of 7 narrow poly(methyl methacrylate) standards with MWs covering the entire MW range of the investigated samples (from 2 to 900 kg.mol -1 ) to perform the logarithm of molecular weight vs. retention time calibration using the peak position calibration method (Eksteen and Pardue, 2003).…”