Modified <scp>DNA</scp> Aptamer Immobilization via Cu(I)‐Stabilizing Ligand‐assisted Azide–Alkyne Cycloaddition for Surface Plasmon Resonance Measurement

Kim, Nak-Hyeon; Lê, Hòa; Yang, Yoon Mee; Byun, Kyung Min; Kim, Tae Woo

doi:10.1002/bkcs.10524

Cited by 3 publications

(1 citation statement)

References 33 publications

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“…One should note, however, that a critical requirement for this functionalization scheme is the introduction of non-native azide groups on any biomolecule of interest. Amine groups are biologically prevalent and, to this benefit, can be converted to azides by an extensive set of strategies ranging from activated ester chemistry to one-pot conversions described in the literature ( 22 , 23 ). Other predominant biological moieties such as hydroxyl and sulfhydryl groups can also be converted to azides using commercially available linkers or direct functional group conversions ( 11 , 24 ).…”

Section: Discussionmentioning

confidence: 99%

Modular, tissue-specific, and biodegradable hydrogel cross-linkers for tissue engineering

et al. 2019

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Synthetic hydrogels are investigated extensively in tissue engineering for their tunable physicochemical properties but are bioinert and lack the tissue-specific cues to produce appropriate biological responses. To introduce tissue-specific biochemical cues to these hydrogels, we have developed a modular hydrogel cross-linker, poly(glycolic acid)–poly(ethylene glycol)–poly(glycolic acid)-di(but-2-yne-1,4-dithiol) (PdBT), that can be functionalized with small peptide-based cues and large macromolecular cues simply by mixing PdBT in water with the appropriate biomolecules at room temperature. Cartilage- and bone-specific PdBT macromers were generated by functionalization with a cartilage-associated hydrophobic N-cadherin peptide, a hydrophilic bone morphogenetic protein peptide, and a cartilage-derived glycosaminoglycan, chondroitin sulfate. These biofunctionalized PdBT macromers can spontaneously cross-link polymers such as poly(N-isopropylacrylamide) to produce rapidly cross-linking, highly swollen, cytocompatible, and hydrolytically degradable hydrogels suitable for mesenchymal stem cell encapsulation. These favorable properties, combined with PdBT’s modular design and ease of functionalization, establish strong potential for its usage in tissue engineering applications.

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