Modified rice bran hemicellulose inhibits vascular endothelial growth factor-induced angiogenesis &lt;i&gt;in vitro&lt;/i&gt; via VEGFR2 and its downstream signaling pathways

Zhu, Xia; Okubo, Aya; Igari, Naoki; Ninomiya, Kentaro; Egashira, Yukari

doi:10.12938/bmfh.16-016

Cited by 8 publications

(6 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that hyaluronan can act as a pro-inflammatory cytokine and induce inflammatory processes in psoriatic arthritis (Hellman et al, 2019). The VEGF signaling pathway plays an important role in psoriasis, and modified hemicellulose suppresses VEGF-induced angiogenesis (Malecic and Young, 2016;Zhu et al, 2017). Chronic inflammation is relevant to alterations of the intestinal microbiota that can be modified with hemicellulose (Konkol et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Deciphering Gut Microbiota Dysbiosis and Corresponding Genetic and Metabolic Dysregulation in Psoriasis Patients Using Metagenomics Sequencing

Xiao

Zhang

Jiang

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

BackgroundIncreasing evidence has shown that alterations in the intestinal microbiota play an important role in the pathogenesis of psoriasis. The existing relevant studies focus on 16S rRNA gene sequencing, but in-depth research on gene functions and comprehensive identification of microbiota is lacking.ObjectivesTo comprehensively identify characteristic gut microbial compositions, genetic functions and relative metabolites of patients with psoriasis and to reveal the potential pathogenesis of psoriasis.MethodsDNA was extracted from the faecal microbiota of 30 psoriatic patients and 15 healthy subjects, and metagenomics sequencing and bioinformatic analyses were performed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) database, cluster of orthologous groups (COG) annotations, and metabolic analyses were used to indicate relative target genes and pathways to reveal the pathogenesis of psoriasis.ResultsCompared with healthy individuals, the gut microbiota of psoriasis patients displayed an alteration in microbial taxa distribution, but no significant difference in microbial diversity. A distinct gut microbial composition in patients with psoriasis was observed, with an increased abundance of the phyla Firmicutes, Actinobacteria and Verrucomicrobia and genera Faecalibacterium, Bacteroides, Bifidobacterium, Megamonas and Roseburia and a decreased abundance of the phyla Bacteroidetes, Euryarchaeota and Proteobacteria and genera Prevotella, Alistipes, and Eubacterium. A total of 134 COGs were predicted with functional analysis, and 15 KEGG pathways, including lipopolysaccharide (LPS) biosynthesis, WNT signaling, apoptosis, bacterial secretion system, and phosphotransferase system, were significantly enriched in psoriasis patients. Five metabolites, hydrogen sulfide (H2S), isovalerate, isobutyrate, hyaluronan and hemicellulose, were significantly dysregulated in the psoriatic cohort. The dysbiosis of gut microbiota, enriched pathways and dysregulated metabolites are relevant to immune and inflammatory response, apoptosis, the vascular endothelial growth factor (VEGF) signaling pathway, gut-brain axis and brain-skin axis that play important roles in the pathogenesis of psoriasis.ConclusionsA clear dysbiosis was displayed in the gut microbiota profile, genetic functions and relative metabolites of psoriasis patients. This study is beneficial for further understanding the inflammatory pathogenesis of psoriasis and could be used to develop microbiome-based predictions and therapeutic approaches.

show abstract

Section: Discussionmentioning

confidence: 99%

Deciphering Gut Microbiota Dysbiosis and Corresponding Genetic and Metabolic Dysregulation in Psoriasis Patients Using Metagenomics Sequencing

Xiao

Zhang

Jiang

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Our data showed that both the administration of BHGZD and the two-BACs-combination of MG and GA dramatically decreased the positive expression of CD31 and VEGFA proteins in the synovial tissues of knee joints in AIA-M rats. Accumulating studies reported that the development of angiogenesis may depend on the proliferation, migration, invasion and lumen formation of HUVEC cells in the intravascular layer [ 28 ]. Consistently, we herein found that BHGZD and the two-BACs-combination not only weakened the migrative and invasive abilities of VEGF-induced HUVEC cells, TNF-α-induced MH7A cells and co-cultured MH7A-induced HUVEC cells, but significantly inhibited the lumen formation ability of VEGF-induced HUVEC cells.…”

Section: Discussionmentioning

confidence: 99%

Extensive preclinical evaluation of combined mangiferin and glycyrrhizic acid for restricting synovial neovascularization in rheumatoid arthritis

Mao,

Yan,

et al. 2023

Chin Med

View full text Add to dashboard Cite

Background Synovial neovascularization promotes rheumatoid arthritis (RA) progression. Baihu guizhi decoction (BHGZD) has a potential in restricting this pathological change of RA. Purpose To identify bioactive compounds (BACs) of BHGZD and to elucidate the underlying mechanisms in restricting synovial neovascularization of RA. Method Through transcriptomic profiling, the chemical profiling of BHGZD and its effective transcriptomic profiling against RA were identified. Then, candidate targets and the corresponding BACs against synovial neovascularization were screened by “disease gene-drug target” interaction network analysis and in silico molecular docking. The binding affinities of candidate BAC-target pairs were verified using surface plasmon resonance, and the pharmacokinetic characteristics of BACs in vivo after BHGZD administration at different time points were detected by Ultra Performance Liquid Chromatography-Mass spectrum/Mass spectrum. After that, in vivo experiments based on adjuvant-induced arthritis (AIA-M) rats, and in vitro experiments based on human umbilical vein endothelial cells (HUVEC) and arthritic synovial fibroblasts (MH7A) were carried out to evaluate the pharmacological effects of BHGZD and the two-BACs-combination, and to verify the associated mechanisms. Result VEGFA/VEGFR2/SRC/PI3K/AKT signal axis was screened as one of the key network targets of BHGZD against synovial neovascularization in RA. Mangiferin (MG) and glycyrrhizic acid (GA) were identified as the representative BACs of BHGZD for their strong binding affinities with components of the VEGFA/VEGFR2/SRC/PI3K/AKT signal axis, and their high exposed quantity in vivo. Both BHGZD and the two-BAC combination of MG and GA were demonstrated to be effective in restricting disease severity, reducing synovial inflammation and decreasing the formation of vascular opacities in AIA-M rats, and also reducing the migrative and invasive activities of HUVEC and MH7A cells and attenuating the lumen formation ability of HUVEC cells significantly. Mechanically, both BHGZD and the two-BAC combination markedly reduced the expression of VEGFA in synovial tissues, the serum levels of VEGF and NO, and the enzymatic activity of eNOS, increased the content of endostatin, and also reversed the abnormal alterations in the VEGFA/VEGFR2/SRC/PI3K/AKT signal axis in vivo and in vitro. Conclusion MG and GA may be the representative BACs of BHGZD for restricting excessive synovial vascularization in RA via regulating VEGFA/VEGFR2/SRC/PI3K/AKT signal axis.

show abstract

“…RBAC can affect new blood vessel growth through the vascular endothelial growth factor (VEGF) pathway. An in vitro study by Zhu et al [ 49 ] demonstrated that RBAC significantly inhibited VEGF-induced tube formation in human umbilical vein endothelial cells co-cultured with human dermal fibroblasts. Furthermore, RBAC also suppressed the VEGF-induced proliferation and migration of human umbilical vein endothelial cells in a dose-dependent manner [ 49 ].…”

Section: Health-promoting Propertiesmentioning

confidence: 99%

“…An in vitro study by Zhu et al [ 49 ] demonstrated that RBAC significantly inhibited VEGF-induced tube formation in human umbilical vein endothelial cells co-cultured with human dermal fibroblasts. Furthermore, RBAC also suppressed the VEGF-induced proliferation and migration of human umbilical vein endothelial cells in a dose-dependent manner [ 49 ]. The anti-angiogenic mechanism of RBAC operates through the reduction of not only the VEGF-induced activation of VEGF receptor 2, but also the downstream proteins of protein kinase B, extracellular signal-regulated protein kinase 1/2, and p38 mitogen-activated protein kinase [ 49 ].…”

Section: Health-promoting Propertiesmentioning

confidence: 99%

“…Furthermore, RBAC also suppressed the VEGF-induced proliferation and migration of human umbilical vein endothelial cells in a dose-dependent manner [ 49 ]. The anti-angiogenic mechanism of RBAC operates through the reduction of not only the VEGF-induced activation of VEGF receptor 2, but also the downstream proteins of protein kinase B, extracellular signal-regulated protein kinase 1/2, and p38 mitogen-activated protein kinase [ 49 ]. Therefore, RBAC can potentially mediate angiogenesis, implicated in the pathological progression of conditions such as cancer, cardiac and limb ischemia, diabetic retinopathy, rheumatoid arthritis, and neoplasms [ 50 ].…”

Section: Health-promoting Propertiesmentioning

confidence: 99%

See 1 more Smart Citation

The Health-Promoting Properties and Clinical Applications of Rice Bran Arabinoxylan Modified with Shiitake Mushroom Enzyme—A Narrative Review

et al. 2021

View full text Add to dashboard Cite

Rice bran arabinoxylan compound (RBAC) is derived from defatted rice bran hydrolyzed with Lentinus edodes mycelial enzyme. It has been marketed as a functional food and a nutraceutical with health-promoting properties. Some research has demonstrated this rice bran derivative to be a potent immunomodulator, which also possesses anti-inflammatory, antioxidant, and anti-angiogenic properties. To date, research on RBAC has predominantly focused on its immunomodulatory action and application as a complementary therapy for cancer. Nonetheless, the clinical applications of RBAC can extend beyond cancer therapy. This article is a narrative review of the research on the potential benefits of RBAC for cancer and other health conditions based on the available literature. RBAC research has shown it to be useful as a complementary treatment for cancer and human immunodeficiency virus infection. It can positively modulate serum glucose, lipid and protein metabolism in diabetic patients. Additionally, RBAC has been shown to ameliorate irritable bowel syndrome and protect against liver injury caused by hepatitis or nonalcoholic fatty liver disease. It can potentially ease symptoms in chronic fatigue syndrome and prevent the common cold. RBAC is safe to consume and has no known side effects at the typical dosage of 2–3 g/day. Nevertheless, further research in both basic studies and human clinical trials are required to investigate the clinical applications, mechanisms, and effects of RBAC.

show abstract

Modified rice bran hemicellulose inhibits vascular endothelial growth factor-induced angiogenesis <i>in vitro</i> via VEGFR2 and its downstream signaling pathways

Cited by 8 publications

References 40 publications

Deciphering Gut Microbiota Dysbiosis and Corresponding Genetic and Metabolic Dysregulation in Psoriasis Patients Using Metagenomics Sequencing

Deciphering Gut Microbiota Dysbiosis and Corresponding Genetic and Metabolic Dysregulation in Psoriasis Patients Using Metagenomics Sequencing

Extensive preclinical evaluation of combined mangiferin and glycyrrhizic acid for restricting synovial neovascularization in rheumatoid arthritis

The Health-Promoting Properties and Clinical Applications of Rice Bran Arabinoxylan Modified with Shiitake Mushroom Enzyme—A Narrative Review

Contact Info

Product

Resources

About