Modified resazurin microtiter assay for in vitro and in vivo assessment of sulfamonomethoxine activity against the fish pathogen Nocardia seriolae

Ismail, Tamer Fawzy; Nakamura, Atsushi; Nakanishi, Kenji; Minami, T.; Murase, Takuya; Yanagi, Soetsu; Itami, Toshiaki; Yoshida, Terutoyo

doi:10.1007/s12562-011-0450-8

Cited by 20 publications

(12 citation statements)

References 16 publications

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“…To date, only two antibiotics, sulfamonomethoxine and sulfisozole sodium, are licensed for treatment of N . seriolae infections in Japan [ 8 , 9 ]. Although these antibiotics are valuable for the control of nocardiosis, there are some concerns about the emergence of antibiotic-resistant strains and environmental impacts.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: The first reference genome sequence of the fish pathogenic Nocardia species

et al. 2017

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Nocardiosis caused by Nocardia seriolae is one of the major threats in the aquaculture of Seriola species (yellowtail; S. quinqueradiata, amberjack; S. dumerili and kingfish; S. lalandi) in Japan. Here, we report the complete nucleotide genome sequence of N. seriolae UTF1, isolated from a cultured yellowtail. The genome is a circular chromosome of 8,121,733 bp with a G+C content of 68.1% that encodes 7,697 predicted proteins. In the N. seriolae UTF1 predicted genes, we found orthologs of virulence factors of pathogenic mycobacteria and human clinical Nocardia isolates involved in host cell invasion, modulation of phagocyte function and survival inside the macrophages. The virulence factor candidates provide an essential basis for understanding their pathogenic mechanisms at the molecular level by the fish nocardiosis research community in future studies. We also found many potential antibiotic resistance genes on the N. seriolae UTF1 chromosome. Comparative analysis with the four existing complete genomes, N. farcinica IFM 10152, N. brasiliensis HUJEG-1 and N. cyriacigeorgica GUH-2 and N. nova SH22a, revealed that 2,745 orthologous genes were present in all five Nocardia genomes (core genes) and 1,982 genes were unique to N. seriolae UTF1. In particular, the N. seriolae UTF1 genome contains a greater number of mobile elements and genes of unknown function that comprise the differences in structure and gene content from the other Nocardia genomes. In addition, a lot of the N. seriolae UTF1-specific genes were assigned to the ABC transport system. Because of limited resources in ocean environments, these N. seriolae UTF1 specific ABC transporters might facilitate adaptation strategies essential for marine environment survival. Thus, the availability of the complete N. seriolae UTF1 genome sequence will provide a valuable resource for comparative genomic studies of N. seriolae isolates, as well as provide new insights into the ecological and functional diversity of the genus Nocardia.

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Section: Introductionmentioning

confidence: 99%

Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: The first reference genome sequence of the fish pathogenic Nocardia species

et al. 2017

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“…These genome databases have revealed new information on N. seriolae epidemiology, pathogenesis, transmission and infection control techniques. Traditionally, the pharmaceutical therapy to overcome fish nocardiosis involves several antibiotics, such as sulfamonomehizol and sulfisizole, which have already been approved in Japan as effective treatments for N. serioale infections (Ismail et al, 2012; Ismail & Yoshida, 2017). However, this effort displayed only partial effectiveness with severe problems of microbial resistance, drug residues and environmental pollution (Ismail & Yoshida, 2017).…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity, diagnosis, prevention strategies and immune response of bacterium Nocardia seriolae : A critical review

Nawaz

Gao

Huang

et al. 2022

Aquaculture Research

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Nocardia seriolae, an opportunistic and facultative intracellular Gram‐positive bacterium, is mainly responsible for nocardiosis in fish. Nocardiosis is a chronic, granulomatous and systemic disease in which lesions are found in the skin as well as several internal organs such as the kidney, liver and spleen with the distinctive nodular granuloma structure. N. seriolae is capable of infecting a wide variety of fish species which poses a devastating impact on the aquaculture sector around the globe, particularly in Asia. Due to the lack of appearance of nocardiosis symptoms at the earlier stages, multiple epidemics of nocardiosis causing high mortality have been recorded. To this end, numerous epidemiological studies have been carried out to characterize and identify Nocardia spp. through several detection techniques, which have favoured the formulation of a multitude of antibiotics and vaccines. After bacterial resistance to antibiotics, nowadays, vaccination has been considered the more appropriate strategy to treat nocardiosis. For valuable information related to N. seriolae and to combat fish nocardiosis, this review extended the existing knowledge, including pathogenesis, virulence characteristics, rapid detection techniques, host immune response and prevention strategies against N. seriolae infection in fish species in aquaculture.

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“…Bacteria require tetrahydrofolate to synthesize purines, thymine nucleotides, and DNA; thus, decreases in tetrahydrofolate inhibit bacterial growth and reproduction (Connor, 1998). Several studies have investigated SMM pharmacokinetics, bioavailability, aquatic animal toxicity, and tissue residues, but few studies have investigated the effects of SMM on the intestinal microecology of aquatic animals (Huang et al, 2014; Ismail et al, 2012; Ueno, 1998; Ueno & Aoki, 1996). In this study, Nile tilapia ( Oreochromis niloticus ) were used as the research object.…”

Section: Introductionmentioning

confidence: 99%

The effect of sulfamonomethoxine treatment on the gut microbiota of Nile tilapia (Oreochromis niloticus)

JunChao

et al. 2020

MicrobiologyOpen

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Modified resazurin microtiter assay for in vitro and in vivo assessment of sulfamonomethoxine activity against the fish pathogen Nocardia seriolae

Cited by 20 publications

References 16 publications

Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: The first reference genome sequence of the fish pathogenic Nocardia species

Analysis of the complete genome sequence of Nocardia seriolae UTF1, the causative agent of fish nocardiosis: The first reference genome sequence of the fish pathogenic Nocardia species

Pathogenicity, diagnosis, prevention strategies and immune response of bacterium Nocardia seriolae : A critical review

The effect of sulfamonomethoxine treatment on the gut microbiota of Nile tilapia (Oreochromis niloticus)

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