Modified protocol for improvement of differentiation potential of menstrual blood‐derived stem cells into adipogenic lineage

Abstract

Presented data suggest an efficient differentiation protocol for in vitro production of MenSC-derived adipocytes. These cells are suggested to be an apt alternative to BMSCs for future stem cell therapy of soft tissue injuries.

Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice

“…MenSCs can grow and adhere in vitro and have been shown to be positive for MSC markers [13]. Under specific conditions, MenSCs also undergo multipotent differentiation into various functional cell types, including adipocytes [14], myocytes [15], osteocytes [16], cardiomyocytes [17], neurocytes [18, 19], endothelial cells [20], pancreatic cells [21], and hepatocytes [22–25]. In autologous cell repair and regeneration, MenSCs present major advantages over other sources of MSCs, including ease of access and the ability to achieve repeated sampling in a non-invasive manner.…”

Human menstrual blood-derived stem cells promote functional recovery in a rat spinal cord hemisection model

“…Then, culture medium was replaced with DMEM/F12 supplemented with 10% FBS and cells were cultured for the next 3 days. Then they were treated with DMEM supplemented with 10% FBS, 1 μM DEX, 10 μg/ml recombinant human insulin, and 60 μM indomethacin (Sigma-Aldrich) up to 12 days 44 . For Oil Red O stain analysis, cells were fixed in 4% formaldehyde and then stained with Oil Red O for 30 min.…”

Endometrial stem/progenitor cells: the first 10 years

“…Cultured ERCs express telomerase reverse transcriptase (hTERT) and demonstrate telomerase activity, as well as typical MSC phenotypic markers (Table IV ), but like eMSCs, they do not express the specific bmMSC marker STRO-1 ( Cui et al , 2007 ; Meng et al , 2007 ; Hida et al , 2008 ; Patel et al , 2008 ; Khanmohammadi et al , 2014 ). Pluripotency marker expression has been demonstrated in ERC, including OCT-4 ( Patel et al , 2008 ; Borlongan et al , 2010 ; Darzi et al , 2012 ; Wu et al , 2014b ), SSEA-4 ( Patel et al , 2008 ; Rossignoli et al , 2013 ) and NANOG ( Borlongan et al , 2010 ).…”

“…ERCs have broad in vitro differentiation capacity (Table V ) and, under appropriate conditions, differentiated into typical mesodermal lineages: adipogenic, chondrogenic, osteogenic ( Meng et al , 2007 ; Patel et al , 2008 ; Darzi et al , 2012 ; Rossignoli et al , 2013 ) and skeletal and cardiac muscle ( Cui et al , 2007 ; Hida et al , 2008 ). Compared with bmMSCs, mesodermal differentiation was less robust for ERCs; however, greater differentiation was achieved for the adipogenic lineage using retinoic acid ( Khanmohammadi et al , 2014 ), the osteogenic lineage with human platelet releasate ( Darzi et al , 2012 ) and cardiomyogenic lineage in SF medium containing thyroxine and insulin ( Ikegami et al , 2010 ). Co-culture of ERCs with mouse foetal cardiomyocytes generated spontaneously beating cells, showing striations and expressing cardiac-specific Troponin 1 ( Hida et al , 2008 ; Khanmohammadi et al , 2014 ).…”

“…Compared with bmMSCs, mesodermal differentiation was less robust for ERCs; however, greater differentiation was achieved for the adipogenic lineage using retinoic acid ( Khanmohammadi et al , 2014 ), the osteogenic lineage with human platelet releasate ( Darzi et al , 2012 ) and cardiomyogenic lineage in SF medium containing thyroxine and insulin ( Ikegami et al , 2010 ). Co-culture of ERCs with mouse foetal cardiomyocytes generated spontaneously beating cells, showing striations and expressing cardiac-specific Troponin 1 ( Hida et al , 2008 ; Khanmohammadi et al , 2014 ). ERCs co-cultured with human nucleus pulposus cells in 2% oxygen differentiated into nucleus pulposus-like cells ( Hu et al , 2014 ).…”