Modified procedures for extraction and analysis of carrageenan applied to the red alga Hypnea musciformis

Knutsen, Svein Halvor; Murano, Erminio; D'Amato, Monica; Toffanin, Renato; Rizzo, Roberto; Paoletti, Sergio

doi:10.1007/bf00003944

Cited by 48 publications

(19 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Based on the structures of the enzyme-degraded κ-carrageenans, the enzyme behaved like a member of the family of κ-carrageenases, which degrade κ-carrageenan by hydrolyzing the internal β(1 3) linkage → s and yield products of the κ-neocarrabiose series [19], though the mechanism of the protein-polysaccharide interaction is still unknown. κ-Neocarratetraose sulfate and κ-neocarrahexaose sulfate were resistant to complete enzymatic degradation, perhaps because the enzyme specificity seems linked to some minimal repeating sequence [20] or other enzymes involved in the breakdown into κ-neocarrabiose sulfate [21].…”

Section: Properties Of κ-Carrageenasementioning

confidence: 99%

A κ-carrageenase from a newly isolated pseudoalteromonas-like bacterium, WZUC10

Zhou

et al. 2008

Biotechnol Bioproc E

View full text Add to dashboard Cite

A bacterial strain able to produce κ-carrageenase, designated WZUC10, was isolated from a live specimen of the red alga mäçÅ~ãáìã=íÉäÑ~áêá~É collected in the East China Sea. The phylogenetic evidence and phenotypic features indicate that this strain belongs to the genus mëÉìÇç~äíÉêçãçå~ë. WZUC10 requires NaCl for growth and κ-carrageenan to induce κ-carrageenase synthesis; galactose and lactose do not induce it. The optimal growth temperature is 23~27°C. The secreted enzyme, which has a molecular mass of 45 kDa, breaks down κ-carrageenan into κ-neocarratetraose sulfate and larger oligosaccharides with a repeating β-D-Galé4S-(1→4)-α-D-AnGalé structure, but cannot degrade κ-neocarratetraose sulfate or κ-neocarrahexaose sulfate into κ-neocarrabiose sulfate. The enzyme retains 90% of its activity after 2 h at 40°C and is completely inactivated after 7.5 min at 70°C. The enzyme's optimal temperature is 30°C and its optimal pH is 7.5. The enzyme-catalyzed reaction follows Michaelis-Menten kinetics, with the Michaelis constant (h m ) and the turnover number (â) being 0.015 mM and 125 s −1 , respectively. WZUC10 produces 50 U/mL κ-carrageenase after cultivation at 25°C for 35 h on a medium containing 80 g/L glucose, 5 g/L corn steep liquor, 3 g/L κ-carrageenan, and 15 g/L NaCl. κ-Neocarratetraose sulfate was prepared simply with precipitation by ethanol:water (5:1, v/v). © KSBB hÉóïçêÇëW=κJÅ~êê~ÖÉÉå~ëÉI PseudoalteromonasI=ëìäÑ~íÉÇ=çäáÖçë~ÅÅÜ~êáÇÉI=κJåÉçÅ~êê~íÉíê~çëÉI=ÄáçÇÉÖê~Ç~íáçåI=ÅÜ~ê~ÅíÉêáò~íáçå= = = = =

show abstract

Section: Properties Of κ-Carrageenasementioning

confidence: 99%

A κ-carrageenase from a newly isolated pseudoalteromonas-like bacterium, WZUC10

Zhou

et al. 2008

Biotechnol Bioproc E

View full text Add to dashboard Cite

show abstract

“…The species Hypnea musciformis (Wulfen) J.V. Lamouroux is economically important as a source of carrageenan, a sulfated polysaccharide extracted from the cell walls, and widely used in the food, cosmetics, and pharmaceutical industries (Knutsen et al 1995). In Brazil, "H. musciformis" is the most common and important species of Hypnea, presenting a wide geographical distribution along the Brazilian coastline (Nunes 2005).…”

Section: Introductionmentioning

confidence: 99%

Description of Hypnea pseudomusciformis sp. nov., a new species based on molecular and morphological analyses, in the context of the H. musciformis complex (Gigartinales, Rhodophyta)

Nauer¹,

Cassano²,

Oliveira³

2014

J Appl Phycol

View full text Add to dashboard Cite

Hypnea musciformis, a red macroalga widely distributed in tropical and subtropical coasts around the world, has great economic importance as a source of carrageenan for industrial production. In this work, the DNA barcode marker COI-5P and the plastid rbcL gene, in addition to morphological studies, were used to investigate this species on the coast of Brazil and compare it with specimens from other countries. A total of 128 sequences were obtained in this study for 100 specimens from Brazil and 15 specimens from other countries, including the type locality in Italy. The divergence between South American sequences and sequences from Italy for H. musciformis was significantly high for both markers, indicating that the specimens found on the Brazilian coast belong to a different species. Considering the data gathered from molecular markers and morphological analysis, the specimens previously identified morphologically as "H. musciformis", "Hypnea nigrescens", and "Hypnea valentiae" collected in Brazil were considered morphological variations of the new species described in this paper, named Hypnea pseudomusciformis Nauer, Cassano & M.C. Oliveira, sp. nov. The identification of specimens based only on morphological characteristics proved to be unsatisfactory for reasons that could be attributed to phenotypic plasticity in this species. Thus, the technique of DNA barcoding, especially with respect to the COI-5P marker, was essential for the identification and definition of species, revealing scenarios that would otherwise be ignored by using only morphological analysis.

show abstract

“…The yields of SP1 and SP2 were approximately 15 and 23% of the mass of the red seaweed dry weight, respectively ( Table 1). The yield of SP2 is comparable to that of hot water extraction followed by cetrimide precipitation of P. deipiens (26%) (Matsuhiro and Urzua 1996), and that of red seaweed Hypnea musciformis (25%) using the same dilute acid extraction method (Knutsen et al 1995). To further purify the crude SP1 and SP2, samples were loaded onto a DEAE-cellulose column and a single symmetrical peak was eluted at approximately 1.4 M NaCl in all cases (data not shown).…”

Section: Resultsmentioning

confidence: 59%

Anticancer Activity of Sulfated Polysaccharides Isolated from the Antarctic Red Seaweed Iridaea cordata

Kim¹,

Kim²,

Koo³

et al. 2016

Ocean and Polar Research

View full text Add to dashboard Cite

: This study aimed to isolate and characterize sulfated polysaccharides (SPs) from Iridaea cordata and evaluate their anticancer activity. SPs of the Antarctic red seaweed were obtained by CaCl 2 (SP1) and ethanol precipitations (SP2) following diluted acid extraction at room temperature. Yields of SP1 and SP2 were approximately 14% and 23%, respectively, of the dry weight of red seaweed. The average molecular mass of the SP1 and SP2 was estimated about 1.84 × 10 3 and 1.42 × 10 3 kDa, respectively, by size-fractionation High-Performance Liquid Chromatography (HPLC). From the High-Performance AnionExchange Chromatography-Pulsed Amperometric Detection (HPAEC-PAD) analysis, the main monosaccharide was galactose with glucose and fucose as minor components. The sulfate content of SP2 (40.4%) was slightly higher than that of SP1 (33.8%). The FT-IR spectra also showed characteristic band of carrageenan-like sulfated polysaccharides. Taken together the SPs are thought to be carrageenan-like sulfated galactan. The polysaccharides (SPs) from I. cordata exhibited weak antitumor activity against PC-3 (prostate cancer), HeLa (cervical cancer), and HT-29 (human colon adenocarcinoma). To our knowledge, this is the first data on biological activity of the Antarctic red seaweed I. cordata.

show abstract

Modified procedures for extraction and analysis of carrageenan applied to the red alga Hypnea musciformis

Cited by 48 publications

References 28 publications

A κ-carrageenase from a newly isolated pseudoalteromonas-like bacterium, WZUC10

A κ-carrageenase from a newly isolated pseudoalteromonas-like bacterium, WZUC10

Description of Hypnea pseudomusciformis sp. nov., a new species based on molecular and morphological analyses, in the context of the H. musciformis complex (Gigartinales, Rhodophyta)

Anticancer Activity of Sulfated Polysaccharides Isolated from the Antarctic Red Seaweed Iridaea cordata

Contact Info

Product

Resources

About