Modified PLGA nanofibers as a nerve regenerator with Schwann cells

Davood, Zolfaghari; Tebyanian, Hamid; Soufdoost, Reza Sayyad; Emamgholi, Askar; Barkhordari, Aref; Herfedoost, Gholam Reza; Kaka, Gholam Reza; Rashidiani, Jamal

doi:10.14715/cmb/2018.64.14.11

Cited by 7 publications

(9 citation statements)

References 35 publications

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“…Further, this decellularized matrix gel also facilitated nerve fiber remyelination by Schwann cells in vitro, indicating that the gel supported the maturation of the Schwann cells [ 150 ]. In addition to using native ECM proteins and their peptide derivatives to functionalize electrospun fibers, researchers have employed biochemical cues not native to the PNS ECM to enhance Schwann cell adhesion, proliferation, elongation, and migration [ 151 , 152 , 153 ]. For example, Feng et al functionalized the surface of aligned poly ( l -lactic acid-co-ε-caprolactone) (PLCL) electrospun fibers with the protein avidin and cultured biotinylated Schwann cells onto them.…”

Section: Peripheral Nervous System Glia and Electrospun Fibersmentioning

confidence: 99%

Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

et al. 2020

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Electrospinning is a fabrication technique used to produce nano- or micro- diameter fibers to generate biocompatible, biodegradable scaffolds for tissue engineering applications. Electrospun fiber scaffolds are advantageous for neural regeneration because they mimic the structure of the nervous system extracellular matrix and provide contact guidance for regenerating axons. Glia are non-neuronal regulatory cells that maintain homeostasis in the healthy nervous system and regulate regeneration in the injured nervous system. Electrospun fiber scaffolds offer a wide range of characteristics, such as fiber alignment, diameter, surface nanotopography, and surface chemistry that can be engineered to achieve a desired glial cell response to injury. Further, electrospun fibers can be loaded with drugs, nucleic acids, or proteins to provide the local, sustained release of such therapeutics to alter glial cell phenotype to better support regeneration. This review provides the first comprehensive overview of how electrospun fiber alignment, diameter, surface nanotopography, surface functionalization, and therapeutic delivery affect Schwann cells in the peripheral nervous system and astrocytes, oligodendrocytes, and microglia in the central nervous system both in vitro and in vivo. The information presented can be used to design and optimize electrospun fiber scaffolds to target glial cell response to mitigate nervous system injury and improve regeneration.

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Section: Peripheral Nervous System Glia and Electrospun Fibersmentioning

confidence: 99%

Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

et al. 2020

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“…When used as a scaffold coating for tissue engineering, PLL mainly improves neuronal adhesion and promotes peripheral nerve repair. Davood Zolfaghari et al [ 96 ] determined that the hydrophobicity of PLGA coated with PLL significantly increased the contact angle studies, and the level of cell proliferation was increased when nanofibers coated with PLL were used.…”

Section: Plga Scaffold Surface-modified Materialsmentioning

confidence: 99%

Surface Modification Progress for PLGA-Based Cell Scaffolds

Yan,

Hua,

Wang

et al. 2024

Polymers

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Poly(lactic-glycolic acid) (PLGA) is a biocompatible bio-scaffold material, but its own hydrophobic and electrically neutral surface limits its application as a cell scaffold. Polymer materials, mimics ECM materials, and organic material have often been used as coating materials for PLGA cell scaffolds to improve the poor cell adhesion of PLGA and enhance tissue adaptation. These coating materials can be modified on the PLGA surface via simple physical or chemical methods, and coating multiple materials can simultaneously confer different functions to the PLGA scaffold; not only does this ensure stronger cell adhesion but it also modulates cell behavior and function. This approach to coating could facilitate the production of more PLGA-based cell scaffolds. This review focuses on the PLGA surface-modified materials, methods, and applications, and will provide guidance for PLGA surface modification.

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“…Then, a volume of 500 μl PBS was added to the sample and washed. Cells were then fi xed by 50 μl of paraformaldehyde solution (the paraformaldehyde solution can be added simultaneously with the antibody) and then the fl uorescence of the samples was examined by fl ow cytometry (18).…”

Section: Evaluation Of Cell Surface Specifi C Markersmentioning

confidence: 99%

Evaluation of the repair of diaphyseal fracture of femoral bone using bone marrow mesenchymal stem cells in nicotine-bearing rat

Riahi¹,

Parivar²,

Baharara³

et al. 2019

BLL

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OBJECTIVE: Following bone fractures during accidents, some patients suffer from poor repair of bone fractures and subsequent aesthetic and psychological problems. One of the treatments is based on transplantation of stem cells (seeded on scaffolds) to the lesion site. Bone marrow stromal cells (BMSCs) are multivalent stem cells which are able to reproduce and differentiate into osteogenic cells. The objective of this study was to evaluate the treatment of bone fractures by means of transplantation of the latter cells in rats. METHODS: In this study, the therapeutic effect of mesenchymal stem cells from bone marrow adipocytes was evaluated in bone fractures. BMSCs were isolated from rat femur. Two sources of differentiated and non-differentiated osteocyte cells were provided and mixed with collagen in order to be transferred to animals divided in three main groups of model: nicotine, non-nicotine and control groups. After four weeks, the repair of the fracture that had been infl icted by a 2-mm drill into the diaphyseal region of the femoral bone was investigated by radiographic tests and histopathologic staining. RESULTS: The radiographic results as well as those of histopathologic staining showed that osteogenesis was more intensive in the non-nicotine group than in the nicotine group with differentiated and non-differentiated osteocyte cells. CONCLUSION: The transplantation of differentiated BMSCs to a bone lesion affected the repair of bone fractures while the nicotine agent played an important role in delaying the bone regeneration (Tab. 1, Fig. 8, Ref. 31).

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Modified PLGA nanofibers as a nerve regenerator with Schwann cells

Cited by 7 publications

References 35 publications

Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

Surface Modification Progress for PLGA-Based Cell Scaffolds

Evaluation of the repair of diaphyseal fracture of femoral bone using bone marrow mesenchymal stem cells in nicotine-bearing rat

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