Modified Model of Sub-Critical Size Cranial Defect in the Rabbit

Abstract

SUMMARY:In medical sciences, any experimental animal model should be reproducible and adequate to the purpose of simulated human physiological response. In bone injury response research, when bone substitutes are in use, it is of primary importance that studied defects fail to heal unless treated with the tissue engineering therapy under study. This failure defines the concept of "critical size defect" (CSD) wich has different limits according to the animal species used and the location of the defect. Although this is a basic concept, when the aim of the study is to investigate the interface Bone-Biomaterial, it is of primary importance to obtain as much contact area as possible. In order to do so, we propose a modified surgical approach to the classical bi-parietal round sub-critical defect model in rabbit vault.

Experimental long bone fracture healing in goats with cockle shell-based calcium carbonate bone paste

“…This supports the present study that in the treated group the gap was filled with a more closely connected bone end, the new bone was further reconstructed, and the diameter tended to be similar to the middle and upper part of the normal femur, while in control group bone gap was visible, gap site was significantly thicker, and bony callus formation was visible to the naked eye. The complete bone healing within 60 days in present study equally agreed with the report of previous researchers following various implants for bone healing in rabbits ( Marques et al, 2010 ; Oh et al, 2005 ) and humans ( Hallman & Thor, 2008 ).…”

Assessment of Bone Healing in Rabbit Calvaria Grafted with Three Different Biomaterials

“…Using standardized defects of 8 mm diameters in the parietal bones of rabbit calvariae allowed large increases in their interface with bone graft materials used in this study. This was a reasonable choice with previously shown experimental results (14). This region was also important because of its similar embryological origin and morphology to the maxilla, as well as its limited anatomic area of mechanical stress and relative stability of the neighboring structures, both of which make it ideal for evaluating osteogenesis induced by biomaterials (15).…”

Comparison of bone regeneration using three demineralized freeze-dried bone allografts: A histological and histomorphometric study in rabbit calvaria

“…[ 2 3 ] Many bone substitutes are introduced every day such as allografts, xenografts and synthetically produced ones. [ 4 ] One of the commonly used substitute is allogenic bone graft. [ 5 ] The use of demineralized freeze-dried bone allograft (DFDBA), whether alone or in combination with other bone substitute, showed significant improvements in bone augmentation procedures.…”