Modified Mild Heat Shock Modality Attenuates Hepatic Ischemia/Reperfusion Injury

Oba, Mariko; Suico, Mary Ann; Morino, Saori; Yano, Shuichiro; Matsuno, Takashi; Koga, Tomoaki; Satō, Takashi; Shuto, Tsuyoshi; Kai, Hirofumi

doi:10.1016/j.jss.2009.03.093

Cited by 20 publications

(21 citation statements)

References 34 publications

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“…Total RNA (1 mg) from each sample was subject to reverse transcription with oligo(dT) primers by PCR kit according to manufacturer's instruction. The synthesized cDNA was used for PCR amplification with the following primers 28,29) : MMP-9, 5Ј-gcacgacgtcttccagtacc-3Ј (For), 5Ј-tcaactcactccgggaactc-3Ј (Rev); MMP-2, 5Ј-ggactatgaccgggataagaaatatg-3Ј (For), 5Ј-gggcaccttctgaatttcca-3Ј (Rev); hypoxanthine-guanine phosophoribosyltransferase (HPRT): 5Ј-gttggatacaggccagactttgttg-3Ј (For), 5Ј-gattcaacttgcgctcatcttaggc-3Ј (Rev). cDNAs were amplified for 30 cycles and each PCR reaction condition was as follows: preparation step at 94°C for 5 min, denaturing step at 94°C for 30 s, annealing step at 56°C for 30 s, and polymerization step at 72°C for 30 s. PCR products were analyzed by agarose gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

.ALPHA.-Solanine Inhibits Human Melanoma Cell Migration and Invasion by Reducing Matrix Metalloproteinase-2/9 Activities

Shih

Chien

et al. 2010

Biological & Pharmaceutical Bulletin

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Glycoalkaloids are the biologically active secondary metabolites found in many plants, such as potatoes. Glycoalkaloids are produced in leaves, roots, tubers and sprouts of the potato plant, and are involved in host plant resistance to bacteria, fungi, viruses, and insects.1) Several reports have showed that glycoalkaloids suppress the growth of cancer cells in human skin, liver, prostate, breast and colon.2-4) aSolanine, a trisaccharide glycoalkaloid, is one of the main steroidal glycoalkaloids in potatoes (Solanum tuberosum). 1)Recent studies have demonstrated that a-solanine inhibits the growth of human colon (HT29), liver (HepG2), cervical (HeLa), lymphoma (U937), and stomach (AGS and KATO III) cancer cells.3,5) a-Solanine has also been shown to induce apoptosis of human colon cancer cells through the inhibition of extracellular signal regulating kinase (ERK) phosphorylation and activation of caspase-3.6) Therefore, a-solanine may possess the potential for cancer chemotherapeutic action.Melanoma is a skin cancer that arises from the transformation of melanocytes, which are normally found in the basal layer of the epidermis. Melanoma accounts for nearly 4% of skin cancer cases but for 74% of all skin cancer mortalities. 7)Melanoma development and progression have been welldescribed as a sequential process. Following transformation of melanocytes, tumors undergo horizontal or radial initial growth phase followed by a subsequent vertical growth phase, in which melanoma cells infiltrate and invade the dermis and exhibit metastatic potential. 8,9) The conversion of benign to malignant melanoma is characterized by genetic alteration and frequent chromosomal abnormalities.10,11) Therefore, malignant melanoma has served as an excellent model for investigating the molecular changes associated with the metastatic phenotype.Cancer metastasis is a highly coordinated multistep process, in which cancer cells degrade the extracellular matrix (ECM), penetrate through the basement membrane of capillary and lymphatic vessels, intravasate, and then invade and grow in new tissue.12) The process of metastasis is promoted by expressing and secreting various proteolytic enzymes that can degrade most ECM components. Matrix metalloproteinases (MMPs), a family of Zn-dependent endopeptidases, are the major proteases participating in tumor cell migration, spreading, tissue invasion and metastasis.13) Several MMPs, such as MMP-2 and MMP-9, contribute to the process of metastasis.14,15) The activation of these enzymes enables the degradation of ECM by cancer cells, allowing their access to the vasculature, as well as their migration, and invasion into the target organ and development of cancer metastasis. 13)In addition to MMPs, the mitogen-activated protein kinase family members (MAPK) are also known to mediate metastasis. MAPK family members participate in numerous signaling cascades that play important regulatory roles in cell growth, apoptosis, differentiation, and metastasis. 16,17) The diverse MAPK members are activated in response...

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Section: Methodsmentioning

confidence: 99%

.ALPHA.-Solanine Inhibits Human Melanoma Cell Migration and Invasion by Reducing Matrix Metalloproteinase-2/9 Activities

Shih

Chien

et al. 2010

Biological & Pharmaceutical Bulletin

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“…Indeed, studies by our group and others have demonstrated that electrical stimulation activates the PI3K-Akt pathway, resulting in the process of wound healing (10) and amelioration of hyperglycemia (11,12). We have also shown that, together with heat shock, which elevates the level of the heat shock protein Hsp72, mild electrical stimulation (MES) 2 attenuates hepatic ischemia/reperfusion injury in mice (13)(14)(15), ameliorates the diabetic phenotype and protects pancreatic ␤-cells in a diabetes mouse model (11,16), reduces inflammatory markers in healthy male subjects (17), and decreases proteinuria and renal inflammation in an Alport syndrome mouse model (18). In the latter disease model, MES was shown to activate not only the PI3K-Akt pathway but also the p38 MAPK signaling pathway (18).…”

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confidence: 96%

Mild Electrical Stimulation at 0.1-ms Pulse Width Induces p53 Protein Phosphorylation and G2 Arrest in Human Epithelial Cells

Fukuda

Suico

Koyama

et al. 2013

Journal of Biological Chemistry

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Background: A controlled approach as opposed to conventional toxic drugs to activate p53 is applicable for tumors and metabolic and inflammatory diseases. Results: A 0.1-ms pulse width mild electrical stimulation (MES) activated p53 function in epithelial cell lines. Conclusion: MES induced p53 phosphorylation via p38 MAPK signaling and G 2 cell cycle arrest without cell death. Significance: MES works as a non-cytotoxic and controllable p53 activator.

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“…Ikeda A et al [10] found serum ALT levels significantly less in rat pre-transplanted liver grafts preserved in the University of Wisconsin (UW) solution containing 5% CO (CO-UW solution) group for 21 hours, than preserved in the control UW group one in Lewis rats. Oba M et al [11] suppressed the hepatic IRinduced release of serum ALT levels three-fold, after heat shock (HS) (42 degrees C) and/or mild electrical stimulation (MES) (12V) for 20min than sham ones in mice. Kim KH et al [12] counted elevated ALT levels in the short-term period (3 hours) in a model of orthotopic rat liver transplantation pretreated 4 days before graft harvesting with interferon regulatory factor-1 (IRF-1) than uninfected control liver graft donors.…”

Section: Discussionmentioning

confidence: 99%

The Antioxidant Drug “U-74389g” Effect on Alanine Aminotransferase Levels

Tsompos¹

2017

JAPLR

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The possible recessing capacity of the antioxidant drug "U-74389g" was studied in a rat model. It included the evaluation of the mean alanine aminotransferase (ALT) levels after induced liver ischemia-reperfusion (IR) injury. The 40 used rats were classified at 2 evaluation endpoints: one of 60 min for groups A and C and one of 120 min for groups B and D after reperfusion. The U-74389g was administered only in groups C and D. The U-74389g administration non-significantly suppressed the ALT levels (p=0.4168). The reperfusion time kept non-significantly elevated the ALT levels (p=0.1309). However, the interaction of U-74389g administration and reperfusion time non-significantly suppressed the ALT levels (p=0.4527). The U-74389g administration non-significantly restored the ALT levels, getting on declining them from significant level to non-significant one.

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Modified Mild Heat Shock Modality Attenuates Hepatic Ischemia/Reperfusion Injury

Cited by 20 publications

References 34 publications

.ALPHA.-Solanine Inhibits Human Melanoma Cell Migration and Invasion by Reducing Matrix Metalloproteinase-2/9 Activities

.ALPHA.-Solanine Inhibits Human Melanoma Cell Migration and Invasion by Reducing Matrix Metalloproteinase-2/9 Activities

Mild Electrical Stimulation at 0.1-ms Pulse Width Induces p53 Protein Phosphorylation and G2 Arrest in Human Epithelial Cells

The Antioxidant Drug “U-74389g” Effect on Alanine Aminotransferase Levels

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