Abstract:3D bioprinting technology displays many advantages for tissue engineering applications, but its utilization is limited by veryfew bioinks available for biofabrication. In this study, a novel type of bioink, which includes three methacryloyl modifiedmannans, was introduced to 3D bioprinting for tissue engineering applications. Yeast mannan (YM) was modified by reactingwith methacrylate anhydride (MA) at different concentrations, and three YM derived bioinks were obtained, which weretermed as YM-MA-1, YM-MA-2 an… Show more
“…The process is also suitable for other water-soluble polysaccharides to be modified by MA. The protocol was adapted from Huang et al. (2021) .…”
Section: Step-by-step Methods Detailsmentioning
confidence: 99%
“…This protocol requires a flashlight to initiate the bioink curing, cells and SD rats to assess the biocompatibility of the bioink. The protocol was adapted from Huang et al. (2021) .…”
Section: Step-by-step Methods Detailsmentioning
confidence: 99%
“…This step describes how to print the YM-MA bioink by a common light-assisted printer. The protocol was adapted from Huang et al. (2021) .…”
Section: Step-by-step Methods Detailsmentioning
confidence: 99%
“…This protocol provides a versatile strategy to develop bioinks of polysaccharides with chemical modification sites such as hydroxyl group. For complete details on the use and execution of this protocol, please refer to Huang et al. (2021) .…”
mentioning
confidence: 99%
“…For complete details on the use and execution of this protocol, please refer to Huang et al. (2021) .…”
“…The process is also suitable for other water-soluble polysaccharides to be modified by MA. The protocol was adapted from Huang et al. (2021) .…”
Section: Step-by-step Methods Detailsmentioning
confidence: 99%
“…This protocol requires a flashlight to initiate the bioink curing, cells and SD rats to assess the biocompatibility of the bioink. The protocol was adapted from Huang et al. (2021) .…”
Section: Step-by-step Methods Detailsmentioning
confidence: 99%
“…This step describes how to print the YM-MA bioink by a common light-assisted printer. The protocol was adapted from Huang et al. (2021) .…”
Section: Step-by-step Methods Detailsmentioning
confidence: 99%
“…This protocol provides a versatile strategy to develop bioinks of polysaccharides with chemical modification sites such as hydroxyl group. For complete details on the use and execution of this protocol, please refer to Huang et al. (2021) .…”
mentioning
confidence: 99%
“…For complete details on the use and execution of this protocol, please refer to Huang et al. (2021) .…”
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