Modified Lendrum’s Technique to Demonstrate Metachromasia of Brain Sand in Pineal Gland

Abstract

Background: Tissue components such as amyloid, mast cell granules and sulfated compounds stain differently from original dye. This is known as metachromasia. Calcium aggregations are known to exhibit metachromasia in certain situations. Local factor which could cause metachromasia is high levels of chondroitin sulfate in tissues undergoing calcification. Pineal gland is one such tissue with calcific concretions in its parenchyma known as Corpora arynecea/ Brain sand.