Modified isolation method of Arcobacter spp. from different environmental and food samples

Abstract

This study provides information on the occurrence of Arcobacter in several types of water and food products of animal origin in the Czech Republic. We processed 190 samples using the modified method, and the occurrence of Arcobacter spp. was confirmed in 36.8 % of these. This total incidence consisted of Arcobacter butzleri (27.3 %), Arcobacter cryaerophilus (8.4 %) and Arcobacter skirrowii (1.1 %). We newly described the common presence of Arcobacter spp. in sewage water in the Czech Republic that is released into waterways after processing in water treatment plants (86.7 %). All the acquired isolates were subject to detailed confirmation with subsequent species classification using multiplex PCR and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In this study, we used a modification of a method using passive filtration of an enriched sample, which could be suitable for the isolation of Arcobacter, especially in combination with Campylobacter selective agar chromogenic medium. Our studies have shown this agar to be quite suited to the isolation of Arcobacter and that it can be an appropriate instrument for accelerating culture diagnostics.

The Use of Two Culturing Methods in Parallel Reveals a High Prevalence and Diversity ofArcobacterspp. in a Wastewater Treatment Plant

“…In previous studies that used different culture media and protocols, the prevalence of Arcobacter spp. from wastewater samples ranged from 40% to 100% [10–14, 18]. When a genotyping method was applied, a high genetic diversity was observed.…”

“…In those studies, Arcobacter spp. were isolated in 40% to 100% of the samples studied, using different culture media and protocols, and were found in 66% to 100% of the samples when direct detection by molecular techniques was used [2, 10–14]. Three studies have investigated the presence of Arcobacter in WWTPs after using different treatments [8–10].…”

Presence of Arcobacter species in pet cats and dogs in the Czech Republic

“…Detection using culture methods is usually carried out after growth under aerobic conditions at 25-30 °C for 4-5 days. Given the demanding growth conditions of arcobacters, however, a standard procedure for isolating and growing arcobacters has not yet been successfully developed (Silha et al 2015). The most reliable method for identifying arcobacters is polymerase chain reaction (PCR; Harmon and Wesley 1997;Houf et al 2000).…”

Aportaciones a la epidemiología de Arcobacter y Helicobacter spp.: Aplicación de métodos moleculares a su detección e identificación en alimentos

“…De las 23 especies del género, 4 se consideran patógenas para humanos y animales: A. butzleri, A. cryaerophilus, A. skirrowii, y A. thereius (Vandamme et al, 2005;Collado y Figueras, 2011;Šilha et al, 2015).…”

“…Aunque el papel de las especies de Arcobacter en enfermedades humanas aún no está bien establecido, A. butzleri, A. cryaerophilus, A. skirrowii y A. thereius se han asociado con enfermedades gastrointestinales, tanto en estudios de población como en casos clínicos (Collado y Figueras, 2011). Estas especies han sido aisladas de muestras fecales de personas con diarrea, y en personas con bacteriemia, endocarditis y peritonitis (Fernández et al, 2004;Houf y Stephan, 2007;Samie et al, 2007;Fera et al, 2010;Šilha et al, 2015). Sin embargo, existen informes sobre la existencia de portadores asintomáticos de especies de Arcobacter, con lo cual persisten las incógnitas sobre el potencial patógeno de este género bacteriano (Calvo et al, 2013).…”