Modified glassy carbon electrode with Nafion/MWNTs as a sensitive voltammetric sensor for the determination of paeonol in pharmaceutical and biological samples

Abstract

A simple, highly sensitive method was reported for directly voltammetric determination of paeonol in drug samples and human biological samples. Nafion/multi-wall carbon nanotubes' (MWNTs) composite film was coated on the glassy carbon electrode. The adsorptive voltammetric behavior of paeonol on the Nafion/MWNTs-modified electrode was investigated using cyclic voltammetry (CV) and differential pulse anodic stripping voltammetry (DPASV). The results indicated that the Nafion/MWNTsmodified electrode could remarkably enhance electrocatalytic activity toward the oxidation of paeonol, and showed an excellent resistance capability toward the electrode passivation. A highly sensitive voltammetric sensor was developed for the detection of paeonol in pharmaceutical and biological samples. Under the optimum conditions, the anodic peak current was proportional to paeonol concentration in the range of 6.0 9 10 -7 -6.0 9 10 -5 M with a detection limit of 4.0 9 10 -7 M. Some kinetic parameters were determined, and multi-step mechanism for oxidation of paeonol was proposed.

Origins, Phytochemistry, Pharmacology, Analytical Methods and Safety of Cortex Moutan (Paeonia suffruticosa Andrew): A Systematic Review

“…Yang Suling et al developed a simple, highly sensitive method using modified glassy carbon electrode with Nafion/multi-wall carbon nanotubes as a sensitive voltammetric sensor to determine the content of paeonol ( 83 ) in several pharmaceutical and biological samples, including CM, LDP and paeonol ( 83 ) spiked LDP, urine, and plasma samples [ 152 ]. This modified electrode was characterized by spaghetti-like porous surface, and it significantly increased the oxidation peak current of paeonol ( 83 ) while reducing the oxidation potential.…”

Highly sensitive electrochemical sensor for paeonol using NMP-exfoliated grapheme-modified electrode

“…The peak potential was 0.91 V, and the peak height was very low, revealing that the oxidation activity of paeonol was very poor on GCE surface. According to the reported results [8,20], the oxidation of paeonol is caused by the phenolic hydroxy group, involving one electron and one proton. However, the oxidation peak shifted negatively to 0.83 V, and the peak currents were improved remarkably on the surface of GS/GCE (Fig.…”

“…In addition, electrochemical sensors were also used for the determination of paeonol owing to high sensitivity, short analysis time, good handling convenience, and low cost. For example, an acetylene black nanoparticle-modified electrode with detection limit of 1.0×10 −7 M [7], and a Nafion/multiwall carbon nanotube composite film-modified electrode with detection limit of 4.0×10 −7 M have been developed for the determination of paeonol [8]. However, to the best of our knowledge, electrochemical determination of paeonol using graphene-modified electrode has not been reported.…”

Application of Carbon Nanotubes Modified Electrode in Pharmaceutical Analysis

“…Therefore, the technique of quantitative determination of paeonol is crucial to the evaluation and popularization of traditional Chinese medicines as well as drug products containing paeonol [22,23]. We have carried out the determination of paeonol in pharmaceutical and biological samples using Nafion/CNTs-modified electrode as a sensitive voltammetric sensor [24]. As shown in figure 4, paeonol exhibited an anodic peak on the Nafion/CNTs/GCE ( Figure 4-a), Nafion/GCE ( Figure 4c) and a bare GCE (Figure 4-b) in 0.1 mol/L phosphate buffer solution (pH 7.0).…”