Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro

Wang, Yifan; Zhang, Jiaqi; Zhang, Beihua; Lu, Mengxiong; Ma, Jing; Liu, Zhihong; Huang, Jinke; Ma, Jinxin; Yang, Xuefei; Wang, Fengyun; Tang, Xudong

doi:10.1016/j.jep.2023.116538

Cited by 12 publications

(3 citation statements)

References 56 publications

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“…Saffron supplementation helped increase antioxidant capacity in UC patients to reduce oxidative stress and inflammation, and decrease disease severity [57]. The Gegen Qinlian decoction (MGQD) restores the expression levels of SOD, GSH, and CAT in UC mice to attenuate DSS-induced oxidative stress injury [58]. In our study, we found that RRTE can restore the intracellular ROS levels to normal in vitro, which can also promote the expression levels of ROS-scavenging enzymes SOD1, SOD2, and GPX2, thus RRTE could exert antioxidant effects in UC mice.…”

Section: Discussionmentioning

confidence: 99%

Rosa Roxburghii Tratt Fruit Extract Prevents Dss-Induced Ulcerative Colitis in Mice by Modulating the Gut Microbiota and the IL-17 Signaling Pathway

Li,

Ling,

Huang

et al. 2023

Nutrients

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Ulcerative colitis (UC) is a non-specific inflammatory bowel illness characterized by intestinal mucosal barrier degradation, inflammation, oxidative damage, and gut microbiota imbalances. Rosa roxburghii Tratt Fruit extract (RRTE) was extracted from Rosa roxburghii Tratt fruit, exhibiting an excellent prevention effect against UC; RRTE could prevent the damage of DSS-induced human normal colonic epithelial (NCM 460) cells, especially in cell viability and morphology, and oxidative damage. Additionally, in UC mice, RRTE could limit the intestinal mucosal barrier by increasing the expression of intestinal tight junction proteins and mucin, reducing inflammation and oxidative damage in colon tissue. More importantly, RRTE can increase the abundance of beneficial bacteria to regulate gut microbiota such as Ruminococcus, Turicibacter, and Parabacteroides, and reduce the abundance of harmful bacteria such as Staphylococcus and Shigella. Furthermore, transcriptomics of colonic mucosal findings point out that the beneficial effect of RRTE on UC could be attributed to the modulation of inflammatory responses such as the IL-17 and TNF signaling pathways. The qPCR results confirm that RRTE did involve the regulation of several genes in the IL-17 signaling pathway. In conclusion, RRTE could prevent DSS-induced damage both in vitro and in vivo.

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Section: Discussionmentioning

confidence: 99%

Rosa Roxburghii Tratt Fruit Extract Prevents Dss-Induced Ulcerative Colitis in Mice by Modulating the Gut Microbiota and the IL-17 Signaling Pathway

Li,

Ling,

Huang

et al. 2023

Nutrients

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“…Meta-analyses have shown that GQD effectively alleviates symptoms in individuals with UC, resulting in decreased Ulcerative Colitis Endoscopic Index of Severity (UCEIS) scores and maintaining a low recurrence rate, all while exhibiting minimal adverse events ( 9 ). Furthermore, additional animal studies have elucidated the mechanisms underlying GQD’s therapeutic effects in alleviating ulcerative colitis, including the reduction of inflammation and oxidative stress, inhibition of the IL-6/JAK2/STAT3 signaling pathway, restoration of the balance between Treg and Th17 cells in colonic tissues, and enhancement of intestinal barrier function ( 10 , 11 ).…”

Section: Introductionmentioning

confidence: 99%

Deciphering the shared mechanisms of Gegen Qinlian Decoction in treating type 2 diabetes and ulcerative colitis via bioinformatics and machine learning

Hu,

Xiong,

et al. 2024

Front. Med.

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BackgroundAlthough previous clinical studies and animal experiments have demonstrated the efficacy of Gegen Qinlian Decoction (GQD) in treating Type 2 Diabetes Mellitus (T2DM) and Ulcerative Colitis (UC), the underlying mechanisms of its therapeutic effects remain elusive.PurposeThis study aims to investigate the shared pathogenic mechanisms between T2DM and UC and elucidate the mechanisms through which GQD modulates these diseases using bioinformatics approaches.MethodsData for this study were sourced from the Gene Expression Omnibus (GEO) database. Targets of GQD were identified using PharmMapper and SwissTargetPrediction, while targets associated with T2DM and UC were compiled from the DrugBank, GeneCards, Therapeutic Target Database (TTD), DisGeNET databases, and differentially expressed genes (DEGs). Our analysis encompassed six approaches: weighted gene co-expression network analysis (WGCNA), immune infiltration analysis, single-cell sequencing analysis, machine learning, DEG analysis, and network pharmacology.ResultsThrough GO and KEGG analysis of weighted gene co-expression network analysis (WGCNA) modular genes and DEGs intersection, we found that the co-morbidity between T2DM and UC is primarily associated with immune-inflammatory pathways, including IL-17, TNF, chemokine, and toll-like receptor signaling pathways. Immune infiltration analysis supported these findings. Three distinct machine learning studies identified IGFBP3 as a biomarker for GQD in treating T2DM, while BACE2, EPHB4, and EPHA2 emerged as biomarkers for GQD in UC treatment. Network pharmacology revealed that GQD treatment for T2DM and UC mainly targets immune-inflammatory pathways like Toll-like receptor, IL-17, TNF, MAPK, and PI3K-Akt signaling pathways.ConclusionThis study provides insights into the shared pathogenesis of T2DM and UC and clarifies the regulatory mechanisms of GQD on these conditions. It also proposes novel targets and therapeutic strategies for individuals suffering from T2DM and UC.

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“…Radix Puerariae is the dried root of the leguminous plant Pueraria lobata , which contains abundant isoflavones and has been widely used in the food and health product industries ( Wang et al, 2022 ). Previous studies have found that water extracts containing Radix Puerariae can increase tight junction protein expression ( Wang et al, 2023 ), lower NF-κB activation, reduce inflammatory factor expression in the colon and relieve oxidative stress ( Li et al, 2016 ). These findings suggest that Radix Puerariae has a beneficial regulatory effect on intestinal barrier function and inflammation.…”

Section: Introductionmentioning

confidence: 99%

Puerarin alleviates inflammation and pathological damage in colitis mice by regulating metabolism and gut microbiota

Zou,

Ding,

et al. 2023

Front. Microbiol.

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Dysbiosis of gut microbiota and metabolic pathway disorders are closely related to the ulcerative colitis. Through network pharmacology, we found that puerarin is a potential ingredient that can improve the crypt deformation and inflammatory infiltration in mice, and decrease the levels of IL-1β, IL-6 and TNF-α significantly. Listeria, Alistipes and P. copri gradually became dominant bacteria in UC mice, which were positively correlated with inflammatory factors. Puerarin effectively improved dysbiosis by reducing the abundance of Alistipes, P. copri and Veillonella, and increasing the level of Desulfovibrionacea. Correlation network and metabolic function prediction analysis of the microbiota showed that they formed a tightly connected network and were widely involved in carbohydrate metabolism and amino acid metabolism. Specifically, we observed significant changes in the tryptophan metabolism pathway in DSS mice, with an increase in the abundance of Bacteroidetes and Enterobacteriaceae involved in tryptophan metabolism. However, this metabolic disorder was alleviated after puerarin treatment, including the reversal of 3-HAA levels and an increase in the abundance of Rhodobacteraceae and Halomonadaceae involved in kynurenine metabolism, as well as a significant increase in the purine metabolite guanosine. In conclusion, our study suggests that puerarin has a good therapeutic effect on UC, which is partially achieved by restoring the composition and abundance of gut microbiota and their metabolism.

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Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro

Cited by 12 publications

References 56 publications

Rosa Roxburghii Tratt Fruit Extract Prevents Dss-Induced Ulcerative Colitis in Mice by Modulating the Gut Microbiota and the IL-17 Signaling Pathway

Rosa Roxburghii Tratt Fruit Extract Prevents Dss-Induced Ulcerative Colitis in Mice by Modulating the Gut Microbiota and the IL-17 Signaling Pathway

Deciphering the shared mechanisms of Gegen Qinlian Decoction in treating type 2 diabetes and ulcerative colitis via bioinformatics and machine learning

Puerarin alleviates inflammation and pathological damage in colitis mice by regulating metabolism and gut microbiota

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