Modified dextran cross-linked electrospun gelatin nanofibres for biomedical applications

Jalaja, K.; Kumar, P.R. Anil; Dey, Tuli; Kundu, Subhas C.; James, Nirmala Rachel

doi:10.1016/j.carbpol.2014.08.023

Cited by 63 publications

(31 citation statements)

References 28 publications

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“…We observe that the total amount of drug release is less in the solution with pH 1.2, compared to higher pH, irrespective of crosslinking time. This may be due to protonation of hydrophilic groups of the polymer matrix in acidic pH, which discourages formation of H-bonds with water molecules resulting in less swelling of the membrane [36]. If the matrix is not swelling much, drug molecules will not get enough osmotic pressure, helping in reducing drug release amount.…”

Section: Effect Of Ph Value Of Release Mediummentioning

confidence: 99%

In-vitro release study of hydrophobic drug using electrospun cross-linked gelatin nanofibers

Laha

Yadav

Majumdar

et al. 2016

Biochemical Engineering Journal

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a b s t r a c tDelivering hydrophobic drug within hydrophilic polymer matrix as carrier is usually a challenge. Here we report the synthesis of gelatin nanofibers by electrospinning, followed by testing them as a potential carrier for oral drug delivery system for a model hydrophobic drug, piperine. Electrospun gelatin nanofibers were crosslinked by exposing to saturated glutaraldehyde (GTA) vapor, to improve their water resistive properties. An exposure of only 6 min was not only adequate to control the early degradation with intact fiber morphology, but also significantly marginalized any adverse effects associated with the use of GTA. Scanning electron microscopy imaging, Fourier transform infrared spectroscopy and thermogravimetric analysis were done to study nanofiber morphology, stability of drug and effect of crosslinking. The pH of release medium was also varied as per the gastrointestinal tract for in-vitro drug release study. Results illustrate good compatibility of hydrophobic drug in gelatin nanofibers with promising controlled drug release patterns by varying crosslinking time and pH of release medium.

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Section: Effect Of Ph Value Of Release Mediummentioning

confidence: 99%

In-vitro release study of hydrophobic drug using electrospun cross-linked gelatin nanofibers

Laha

Yadav

Majumdar

et al. 2016

Biochemical Engineering Journal

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“…23,[26][27][28] Several groups have used electrospinning to fabricate gelatin-based cell culture substrates, investigating not only the influence of mechanical characteristics on cell migration and differentiation but also on the biocompatibility/viability effects of different cross-linking agents. [29][30][31] Building upon these works, we set out to fabricate electrospun gelatin mats that optimally support the formation of astrocytes and endothelial cells for the production of a bilayer BBB model in a transwell format. Here we describe the formation of gelatin 'biopaper" membranes, attachment to cell culture inserts, and culture conditions for a transwell BBB model based on this substrate.…”

Section: Introductionmentioning

confidence: 99%

Electrospun gelatin biopapers as substrate for in vitro bilayer models of blood−brain barrier tissue

Bischel

Coneski

Lundin

et al. 2016

J Biomedical Materials Res

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Gaining a greater understanding of the blood-brain barrier (BBB) is critical for improvement in drug delivery, understanding pathologies that compromise the BBB, and developing therapies to protect the BBB. In vitro human tissue models are valuable tools for studying these issues. The standard in vitro BBB models use commercially available cell culture inserts to generate bilayer co-cultures of astrocytes and endothelial cells (EC). Electrospinning can be used to produce customized cell culture substrates with optimized material composition and mechanical properties with advantages over off-the-shelf materials. Electrospun gelatin is an ideal cell culture substrate because it is a natural polymer that can aid cell attachment and be modified and degraded by cells. Here, we have developed a method to produce cell culture inserts with electrospun gelatin "biopaper" membranes. The electrospun fiber diameter and cross-linking method were optimized for the growth of primary human endothelial cell and primary human astrocyte bilayer co-cultures to model human BBB tissue. BBB co-cultures on biopaper were characterized via cell morphology, trans-endothelial electrical resistance (TEER), and permeability to FITC-labeled dextran and compared to BBB co-cultures on standard cell culture inserts. Over longer culture periods (up to 21 days), cultures on the optimized electrospun gelatin biopapers were found to have improved TEER, decreased permeability, and permitted a smaller separation between co-cultured cells when compared to standard PET inserts.

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“…Sample powder was mixed with KBr with sample to KBr ratio of 1:100. [17] Differential scanning calorimetry analysis Investigation of thermal behaviour of nanofibres is important to evaluate their stability under different temperatures. Thermal analysis of the fibres was studied by differential scanning calorimetry (DSC) (TA instruments, model DSC 204).…”

Section: Morphological Studymentioning

confidence: 99%

“…The weak bands in the calculated spectrum at 1660 and 1570 cm −1 theoretically correspond to symmetric and asymmetric aromatic ring stretches, respectively. [14,17,31] …”

Section: Xrd Analysismentioning

confidence: 99%

Characteristics of vitamin E-loaded nanofibres from dextran

Fathi

Nasrabadi

Varshosaz

2017

International Journal of Food Properties

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In this research, dextran nanofibres were produced as novel carriers for entrapment of vitamin E. Morphological studies indicated that developed fine fibres were in the nano range without spherical beads. Thermal behaviour, chemical structure, and crystalline structure of vitamin E-loaded nanofibres were analysed by differential scanning calorimetry, Fourier transform infrared spectroscopy, and X-ray diffraction which showed that the vitamin was well incorporated within the amorphous structure of fibres without chemical interactions. Nanofibres were used for fortification of cheese. Sensory analysis of the fortified product was performed which showed better acceptability and texture of cheese containing nanofibres in comparison to blank and direct fortified samples. Nanofibres showed the capability of holding water and thus increasing cheese firmness. The release of vitamin E in gastrointestinal media showed that about 14% of vitamin could be released in gastric media and about 30% released in intestinal media. Finally, the results revealed that electrospinning can be used for production of dextran ultrathin fibres to entrap hydrophobic compounds with high potential for design of novel functional foods. ARTICLE HISTORY

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Modified dextran cross-linked electrospun gelatin nanofibres for biomedical applications

Cited by 63 publications

References 28 publications

In-vitro release study of hydrophobic drug using electrospun cross-linked gelatin nanofibers

In-vitro release study of hydrophobic drug using electrospun cross-linked gelatin nanofibers

Electrospun gelatin biopapers as substrate for in vitro bilayer models of blood−brain barrier tissue

Characteristics of vitamin E-loaded nanofibres from dextran

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