Modified Carba NP test for the detection of carbapenemase production in gram-negative rods: optimized handling of multiple samples

Campana, Eloiza Helena; Chuster, Stephanie Gomes; Silva, Isadora R. da; Paschoal, Raphael P.; Bonelli, Raquel Regina; Moreira, Beatriz Meurer; Picão, Renata Cristina

doi:10.1016/j.bjm.2016.09.015

Cited by 10 publications

(7 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…38 Similar reports of low sensitivity for the Carba NP test have been reported. 21,22,39 In contrast to the Carba NP test that failed to detect six isolates that were genotypically confirmed to carry at least one carbapenemase gene, the present study revealed that MHT was more sensitive than the Carba NP test, based on failure of MHT to detect only five isolates that were genotypically confirmed to harbor at least one carbapenemase gene.…”

Section: Discussioncontrasting

confidence: 83%

“…Early and rapid detection of these isolates is crucial for initiating effective therapeutic options as early as possible in order to achieve desirable outcomes, especially for serious infections and to prevent the spread of these XDR isolates, 21,22 based on that, the current study used three phenotypic methods for detection of carbapenemase production among CRKP clinical isolates: (i) MHT (ii), Carb NP test, and (iii) CDST.…”

Section: Discussionmentioning

confidence: 99%

“…All CRKP isolates were investigated for carbapenemase production using the Carba NP test as previously described 21,22 in which both CNP test solutions (A) and (B) were prepared. Test results were considered to be positive when CNP solution (B) was converted from red to yellow color, while CNP solution (A) remained red as shown in Figure 1.…”

Section: Carba Nordmann/poirel (Cnp) Testmentioning

confidence: 99%

See 2 more Smart Citations

The First Egyptian Report Showing the Co-Existence of blaNDM-25, blaOXA-23, blaOXA-181, and blaGES-1 Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR

El-Badawy¹,

El‐Far²,

Althobaiti³

et al. 2020

IDR

View full text Add to dashboard Cite

Background and Objective: The emergence of carbapenem-resistant K. pneumoniae (CRKP) continues to escalate and is alarming because of the emergence of pan drugresistant strains. The objective of this study was to investigate the existence of 12 carbapenemase genes among CRKP clinical isolates. Methods: Ninety-six Klebsiella spp. clinical isolates were collected. The isolates were identified phenotypically and genotypically. These isolates were screened for susceptibility to 24 different antibiotics. The modified Hodge test (MHT) and the Carba Nordmann/Poirel (NP) test were used to phenotypically screen carbapenem-resistant strains for carbapenemase production. Phenotypic characterization of carbapenemases was performed using the combined disk synergy test (CDST). Additionally, the presence of 12 carbapenemase genes in CRKP isolates was investigated. The DNA sequence of bla NDM and bla GES genes was determined. The BOX-PCR technique was used to determine the clonal relationship between CRKP isolates. Results: All carbapenem-resistant isolates were related to K. pneumoniae. Susceptibility testing showed that 19.79% (19/96) of the collected isolates were carbapenem-resistant. Of the CRKP isolates, 68.42% (13/19) tested positive for the MHT and Carba NP test. CDST showed that 42.11% (8/19), 63.16% (12/19), 47.37% (9/19), and 73.68% (14/19) of the CRKP isolates tested positive for the inhibitory effect of clavulanic acid, sulbactam, phenylboronic acid, and tazobactam, respectively, while 84.21% (16/19) and 68.42% (13/16) tested positive for the inhibitory effect of EDTA and mercaptopropionic acid, respectively. It was found that 10.53% (2/19) of the isolates tested positive for the inhibitory effect of sodium chloride. Molecular investigation of carbapenemases showed that 26.32% (5/19), 73.68% (14/19), 21.05% (4/19), 10.53% (2/19), and 5.26% (1/19) of the isolates tested positive for bla NDM , bla OXA-48 , bla OXA-181 , bla OXA-51 , and bla OXA-23 , respectively. None of the isolates tested positive for bla OXA-40 and bla OXA-58. Two allelic variants of bla NDM (bla NDM-1 and bla NDM-25) were detected. BOX-PCR revealed high clonal relatedness between CRKP isolates. Conclusion: MHT was more sensitive than Carba NP test for evaluating carbapenemase production and class D carbapenemase genes were the most prevalent of the 12 carbapenemase genes that were evaluated.

show abstract

Section: Discussioncontrasting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Carba Nordmann/poirel (Cnp) Testmentioning

confidence: 99%

See 1 more Smart Citation

The First Egyptian Report Showing the Co-Existence of blaNDM-25, blaOXA-23, blaOXA-181, and blaGES-1 Among Carbapenem-Resistant K. pneumoniae Clinical Isolates Genotyped by BOX-PCR

El-Badawy¹,

El‐Far²,

Althobaiti³

et al. 2020

IDR

View full text Add to dashboard Cite

show abstract

“…Species identification was determined primarily with an automated VITEK®2 AST-16 Gram-negative susceptibility card (bioMérieux, Marcy-l’Étoile, France) and confirmed by matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). Investigation of carbapenemase production in the routine hospital laboratory procedure using the Modified Hodge Test (MHT) [ 37 ] and Carba NP test [ 38 ] showed that EBSI041 was resistant to carbapenems. We tested EBSI041 for carbapenems-resistant genes and confirmed that it carried bla OXA-48 and bla KPC-2 .…”

Section: Methodsmentioning

confidence: 99%

Carriage of distinct blaKPC-2 and blaOXA-48 plasmids in a single ST11 hypervirulent Klebsiella pneumoniae isolate in Egypt

Yang

Ahmed

et al. 2022

BMC Genomics

View full text Add to dashboard Cite

Background Carbapenem-resistant hypervirulent K. pneumoniae (CR-hvKP) causes serious infections with significant morbidity and mortality. However, the epidemiology and transmission mechanisms of CR-hvKP and the corresponding carbapenem-resistant plasmids require further investigation. Herein, we have characterized an ST11 K. pneumoniae strain EBSI041 from the blood sample encoding both hypervirulence and carbapenem resistance phenotypes from a patient in Egypt. Results K. pneumoniae strain EBSI041 showed multidrug-resistance phenotypes, where it was highly resistant to almost all tested antibiotics including carbapenems. And hypervirulence phenotypes of EBSI041 was confirmed by the model of Galleria mellonella infection. Whole-genome sequencing analysis showed that the hybrid plasmid pEBSI041-1 carried a set of virulence factors rmpA, rmpA2, iucABCD and iutA, and six resistance genes aph(3′)-VI, armA, msr(E), mph(E), qnrS, and sul2. Besides, blaOXA-48 and blaSHV-12 were harboured in a novel conjugative IncL-type plasmid pEBSI041-2. The blaKPC-2-carrying plasmid pEBSI041-3, a non-conjugative plasmid lacking the conjugative transfer genes, could be transferred with the help of pEBSI041-2, and the two plasmids could fuse into a new plasmid during co-transfer. Moreover, the emergence of the p16HN-263_KPC-like plasmids is likely due to the integration of pEBSI041-3 and pEBSI041-4 via IS26-mediated rearrangement. Conclusion To the best of our knowledge, this is the first report on the complete genome sequence of KPC-2- and OXA-48-coproducing hypervirulent K. pneumoniae from Egypt. These results give new insights into the adaptation and evolution of K. pneumoniae during nosocomial infections.

show abstract

“…A study by Hamid El Abd et al concluded the sensitivity and specificity to be 100% for the detection of both KPC and MBL producers. Another study carried out by Campana et al reported 73.1% sensitivity and 100% specificity. These studies support the current study, concluding 91.7% sensitivity and 96.7% specificity.…”

Section: Discussionmentioning

confidence: 99%

Modified Carba NP Test: Simple and rapid method to differentiate KPC‐ and MBL‐producing Klebsiella species

Kumar

Singh

Beniwal

et al. 2018

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

Modified Carba NP test for the detection of carbapenemase production in gram-negative rods: optimized handling of multiple samples

Cited by 10 publications

References 14 publications

<p>The First Egyptian Report Showing the Co-Existence of <em>bla</em><sub>NDM-25</sub>, <em>bla</em><sub>OXA-23</sub>, <em>bla</em><sub>OXA-181</sub>, and <em>bla</em><sub>GES-1</sub> Among Carbapenem-Resistant <em>K. pneumoniae</em> Clinical Isolates Genotyped by BOX-PCR</p>

<p>The First Egyptian Report Showing the Co-Existence of <em>bla</em><sub>NDM-25</sub>, <em>bla</em><sub>OXA-23</sub>, <em>bla</em><sub>OXA-181</sub>, and <em>bla</em><sub>GES-1</sub> Among Carbapenem-Resistant <em>K. pneumoniae</em> Clinical Isolates Genotyped by BOX-PCR</p>

Carriage of distinct blaKPC-2 and blaOXA-48 plasmids in a single ST11 hypervirulent Klebsiella pneumoniae isolate in Egypt

Modified Carba NP Test: Simple and rapid method to differentiate KPC‐ and MBL‐producing Klebsiella species

Contact Info

Product

Resources

About