Modified Capsule PCR Multiplex Typing System for &lt;i&gt;Campylobacter jejuni&lt;/i&gt; Penner Types in Japan

Konno, Takayuki; Yamada, Keiko; Akase, Satoru; Sakata, Junko; Obane, Noriko; Mori, Misato; Yokoyama, Keiko; Yamamoto, Shouji; Asakura, Hiroshi

doi:10.5803/jsfm.38.123

Cited by 1 publication

(1 citation statement)

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“…jejuni was determined by antisera, commercially available from Denka (Tokyo, Japan) [ 31 ]. Penner genetic typing was conducted according to the modified Capsule PCR multiplex typing system developed by Konno et al [ 32 ], of which the original scheme was described by Poly et al [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Proteotyping of Campylobacter jejuni by MALDI-TOF MS and Strain Solution Version 2 Software

et al. 2023

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Identification of microorganisms by MALDI-TOF MS has become a popular method in the past 20 years. Strain Solution ver. 2 software appended with MALDI-TOF MS enables accurate discrimination of serotypes and strains beyond the genus and species level by creating a theoretical mass-based database. In this study, we constructed a theoretical mass database with the validated biomarkers to proteotype Campylobacter jejuni. Using 10 strains belonging to Campylobacter spp. available from culture collections and 41 Campylobacter jejuni strains isolated from humans and foods, the ribosomal protein subunits L36, L32, S14, L24, L23, L7/L12, and S11 could be selected as the effective biomarkers for the proteotyping of C. jejuni at MALDI-TOF MS. An accurate database of their theoretical mass-based values was constructed by matching these gene DNA sequences and the observed mass peaks. We attempted to automatically classify 41 strains isolated from nature using this database and Strain Solution ver. 2 software, and 38 strains (93%) were correctly classified into the intended group based on the theoretical mass-based values. Thus, the seven biomarkers found in this study and Strain Solution ver. 2 are promising for the proteotyping of C. jejuni by MALDI-TOF MS.

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Section: Methodsmentioning

confidence: 99%